Evidence of SARS-CoV-2 spike protein on retrieved thrombi from COVID-19 patients

The pathophysiology of COVID-19-associated coagulopathy is complex and not fully understood. SARS-CoV-2 spike protein (SP) may activate platelets and interact with fibrin(ogen). We aimed to investigate whether isolated SP can be present in clots retrieved in COVID-19 patients with acute ischemic str...

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Autores principales: De Michele, Manuela, d’Amati, Giulia, Leopizzi, Martina, Iacobucci, Marta, Berto, Irene, Lorenzano, Svetlana, Mazzuti, Laura, Turriziani, Ombretta, Schiavo, Oscar G., Toni, Danilo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Correspondence
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9380658/
https://www.ncbi.nlm.nih.gov/pubmed/35974404
http://dx.doi.org/10.1186/s13045-022-01329-w
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author De Michele, Manuela
d’Amati, Giulia
Leopizzi, Martina
Iacobucci, Marta
Berto, Irene
Lorenzano, Svetlana
Mazzuti, Laura
Turriziani, Ombretta
Schiavo, Oscar G.
Toni, Danilo
author_facet De Michele, Manuela
d’Amati, Giulia
Leopizzi, Martina
Iacobucci, Marta
Berto, Irene
Lorenzano, Svetlana
Mazzuti, Laura
Turriziani, Ombretta
Schiavo, Oscar G.
Toni, Danilo
author_sort De Michele, Manuela
collection PubMed
description The pathophysiology of COVID-19-associated coagulopathy is complex and not fully understood. SARS-CoV-2 spike protein (SP) may activate platelets and interact with fibrin(ogen). We aimed to investigate whether isolated SP can be present in clots retrieved in COVID-19 patients with acute ischemic stroke (by mechanical thrombectomy) and myocardial infarction. In this pilot study, we could detect SP, but not nucleocapsid protein, on platelets of COVID-19 patients’ thrombi. In addition, in all three COVID-19 thrombi analyzed for molecular biology, no SARS-CoV-2 RNA could be detected by real-time polymerase chain reaction. These data could support the hypothesis that free SP, besides the whole virus, may be the trigger of platelet activation and clot formation in COVID-19. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13045-022-01329-w.
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spelling pubmed-93806582022-08-17 Evidence of SARS-CoV-2 spike protein on retrieved thrombi from COVID-19 patients De Michele, Manuela d’Amati, Giulia Leopizzi, Martina Iacobucci, Marta Berto, Irene Lorenzano, Svetlana Mazzuti, Laura Turriziani, Ombretta Schiavo, Oscar G. Toni, Danilo J Hematol Oncol Correspondence The pathophysiology of COVID-19-associated coagulopathy is complex and not fully understood. SARS-CoV-2 spike protein (SP) may activate platelets and interact with fibrin(ogen). We aimed to investigate whether isolated SP can be present in clots retrieved in COVID-19 patients with acute ischemic stroke (by mechanical thrombectomy) and myocardial infarction. In this pilot study, we could detect SP, but not nucleocapsid protein, on platelets of COVID-19 patients’ thrombi. In addition, in all three COVID-19 thrombi analyzed for molecular biology, no SARS-CoV-2 RNA could be detected by real-time polymerase chain reaction. These data could support the hypothesis that free SP, besides the whole virus, may be the trigger of platelet activation and clot formation in COVID-19. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13045-022-01329-w. BioMed Central 2022-08-16 /pmc/articles/PMC9380658/ /pubmed/35974404 http://dx.doi.org/10.1186/s13045-022-01329-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Correspondence
De Michele, Manuela
d’Amati, Giulia
Leopizzi, Martina
Iacobucci, Marta
Berto, Irene
Lorenzano, Svetlana
Mazzuti, Laura
Turriziani, Ombretta
Schiavo, Oscar G.
Toni, Danilo
Evidence of SARS-CoV-2 spike protein on retrieved thrombi from COVID-19 patients
title Evidence of SARS-CoV-2 spike protein on retrieved thrombi from COVID-19 patients
title_full Evidence of SARS-CoV-2 spike protein on retrieved thrombi from COVID-19 patients
title_fullStr Evidence of SARS-CoV-2 spike protein on retrieved thrombi from COVID-19 patients
title_full_unstemmed Evidence of SARS-CoV-2 spike protein on retrieved thrombi from COVID-19 patients
title_short Evidence of SARS-CoV-2 spike protein on retrieved thrombi from COVID-19 patients
title_sort evidence of sars-cov-2 spike protein on retrieved thrombi from covid-19 patients
topic Correspondence
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9380658/
https://www.ncbi.nlm.nih.gov/pubmed/35974404
http://dx.doi.org/10.1186/s13045-022-01329-w
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