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Inhibition of itch by neurokinin 1 receptor (Tacr1) -expressing ON cells in the rostral ventromedial medulla in mice
The rostral ventromedial medulla (RVM) is important in descending modulation of spinal nociceptive transmission, but it is unclear if the RVM also modulates spinal pruriceptive transmission. RVM ON cells are activated by noxious algesic and pruritic stimuli and are pronociceptive. Many RVM-spinal pr...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9381038/ https://www.ncbi.nlm.nih.gov/pubmed/35972457 http://dx.doi.org/10.7554/eLife.69626 |
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author | Follansbee, Taylor Domocos, Dan Nguyen, Eileen Nguyen, Amanda Bountouvas, Aristea Velasquez, Lauren Iodi Carstens, Mirela Takanami, Keiko Ross, Sarah E Carstens, Earl |
author_facet | Follansbee, Taylor Domocos, Dan Nguyen, Eileen Nguyen, Amanda Bountouvas, Aristea Velasquez, Lauren Iodi Carstens, Mirela Takanami, Keiko Ross, Sarah E Carstens, Earl |
author_sort | Follansbee, Taylor |
collection | PubMed |
description | The rostral ventromedial medulla (RVM) is important in descending modulation of spinal nociceptive transmission, but it is unclear if the RVM also modulates spinal pruriceptive transmission. RVM ON cells are activated by noxious algesic and pruritic stimuli and are pronociceptive. Many RVM-spinal projection neurons express the neurokinin-1 receptor (Tacr1), and ON-cells are excited by local administration of substance P (SP). We hypothesized that Tacr1-expressing RVM ON cells exert an inhibitory effect on itch opposite to their pronociceptive action. Intramedullary microinjection of SP significantly potentiated RVM ON cells and reduced pruritogen-evoked scratching while producing mild mechanical sensitization. Chemogenetic activation of RVM Tacr1-expressing RVM neurons also reduced acute pruritogen-evoked scratching. Optotagging experiments confirmed RVM Tacr1-expressing neurons to be ON cells. We conclude that Tacr1-expressing ON cells in RVM play a significant role in the modulation of pruriceptive transmission. |
format | Online Article Text |
id | pubmed-9381038 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-93810382022-08-17 Inhibition of itch by neurokinin 1 receptor (Tacr1) -expressing ON cells in the rostral ventromedial medulla in mice Follansbee, Taylor Domocos, Dan Nguyen, Eileen Nguyen, Amanda Bountouvas, Aristea Velasquez, Lauren Iodi Carstens, Mirela Takanami, Keiko Ross, Sarah E Carstens, Earl eLife Neuroscience The rostral ventromedial medulla (RVM) is important in descending modulation of spinal nociceptive transmission, but it is unclear if the RVM also modulates spinal pruriceptive transmission. RVM ON cells are activated by noxious algesic and pruritic stimuli and are pronociceptive. Many RVM-spinal projection neurons express the neurokinin-1 receptor (Tacr1), and ON-cells are excited by local administration of substance P (SP). We hypothesized that Tacr1-expressing RVM ON cells exert an inhibitory effect on itch opposite to their pronociceptive action. Intramedullary microinjection of SP significantly potentiated RVM ON cells and reduced pruritogen-evoked scratching while producing mild mechanical sensitization. Chemogenetic activation of RVM Tacr1-expressing RVM neurons also reduced acute pruritogen-evoked scratching. Optotagging experiments confirmed RVM Tacr1-expressing neurons to be ON cells. We conclude that Tacr1-expressing ON cells in RVM play a significant role in the modulation of pruriceptive transmission. eLife Sciences Publications, Ltd 2022-08-16 /pmc/articles/PMC9381038/ /pubmed/35972457 http://dx.doi.org/10.7554/eLife.69626 Text en © 2022, Follansbee et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Follansbee, Taylor Domocos, Dan Nguyen, Eileen Nguyen, Amanda Bountouvas, Aristea Velasquez, Lauren Iodi Carstens, Mirela Takanami, Keiko Ross, Sarah E Carstens, Earl Inhibition of itch by neurokinin 1 receptor (Tacr1) -expressing ON cells in the rostral ventromedial medulla in mice |
title | Inhibition of itch by neurokinin 1 receptor (Tacr1) -expressing ON cells in the rostral ventromedial medulla in mice |
title_full | Inhibition of itch by neurokinin 1 receptor (Tacr1) -expressing ON cells in the rostral ventromedial medulla in mice |
title_fullStr | Inhibition of itch by neurokinin 1 receptor (Tacr1) -expressing ON cells in the rostral ventromedial medulla in mice |
title_full_unstemmed | Inhibition of itch by neurokinin 1 receptor (Tacr1) -expressing ON cells in the rostral ventromedial medulla in mice |
title_short | Inhibition of itch by neurokinin 1 receptor (Tacr1) -expressing ON cells in the rostral ventromedial medulla in mice |
title_sort | inhibition of itch by neurokinin 1 receptor (tacr1) -expressing on cells in the rostral ventromedial medulla in mice |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9381038/ https://www.ncbi.nlm.nih.gov/pubmed/35972457 http://dx.doi.org/10.7554/eLife.69626 |
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