Acinetobacter baumannii, Klebsiella pneumoniae and Elizabethkingia miricola isolated from wastewater have biodegradable activity against fluoroquinolone
Ciprofloxacin (CIP) and levofloxacin (LEV), widely used fluoroquinolone antibiotics, are often found in sewage from the sewage treatment plants and marine environment. In this study, CIP and LEV biodegrading bacterial consortia were obtained from industrial wastewater. Microorganisms in these consor...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9381475/ https://www.ncbi.nlm.nih.gov/pubmed/35972564 http://dx.doi.org/10.1007/s11274-022-03367-5 |
_version_ | 1784769087185879040 |
---|---|
author | Shaker, Reham Alaa Eldin Nagy, Yosra Ibrahim Adly, Mina E. Khattab, Rania Abdelmonem Ragab, Yasser M. |
author_facet | Shaker, Reham Alaa Eldin Nagy, Yosra Ibrahim Adly, Mina E. Khattab, Rania Abdelmonem Ragab, Yasser M. |
author_sort | Shaker, Reham Alaa Eldin |
collection | PubMed |
description | Ciprofloxacin (CIP) and levofloxacin (LEV), widely used fluoroquinolone antibiotics, are often found in sewage from the sewage treatment plants and marine environment. In this study, CIP and LEV biodegrading bacterial consortia were obtained from industrial wastewater. Microorganisms in these consortia were identified as Acinetobacter baumannii (A. baumannii), Klebsiella pneumoniae (K. pneumoniae) and Elizabethkingia miricola (E. miricola). The impacts of the critical operating parameters on the elimination of CIP and LEV by bacterial consortia have been investigated and optimized to achieve the maximum levels of CIP and LEV biodegradation. Using liquid chromatography with tandem mass spectrometry (LC-MS-MS), possible degradation pathways for CIP and LEV were suggested by analyzing the intermediate degradation products. The role of the enzymes fluoroquinolone-acetylating aminoglycoside (6′-N-acetyltransferase) and cytochrome P450 (CYP450) in the breakdown of fluoroquinolones (FQs) was investigated as well. According to our findings, various biodegradation mechanisms have been suggested, including cleavage of piperazine ring, substitution of F atom, hydroxylation, decarboxylation, and acetylation, as the main biotransformation reactions. This study discovers the ability of non-reported bacterial strains to biodegrade both CIP and LEV as a sole carbon source, providing new insights into the biodegradation of CIP and LEV. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11274-022-03367-5. |
format | Online Article Text |
id | pubmed-9381475 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-93814752022-08-18 Acinetobacter baumannii, Klebsiella pneumoniae and Elizabethkingia miricola isolated from wastewater have biodegradable activity against fluoroquinolone Shaker, Reham Alaa Eldin Nagy, Yosra Ibrahim Adly, Mina E. Khattab, Rania Abdelmonem Ragab, Yasser M. World J Microbiol Biotechnol Research Ciprofloxacin (CIP) and levofloxacin (LEV), widely used fluoroquinolone antibiotics, are often found in sewage from the sewage treatment plants and marine environment. In this study, CIP and LEV biodegrading bacterial consortia were obtained from industrial wastewater. Microorganisms in these consortia were identified as Acinetobacter baumannii (A. baumannii), Klebsiella pneumoniae (K. pneumoniae) and Elizabethkingia miricola (E. miricola). The impacts of the critical operating parameters on the elimination of CIP and LEV by bacterial consortia have been investigated and optimized to achieve the maximum levels of CIP and LEV biodegradation. Using liquid chromatography with tandem mass spectrometry (LC-MS-MS), possible degradation pathways for CIP and LEV were suggested by analyzing the intermediate degradation products. The role of the enzymes fluoroquinolone-acetylating aminoglycoside (6′-N-acetyltransferase) and cytochrome P450 (CYP450) in the breakdown of fluoroquinolones (FQs) was investigated as well. According to our findings, various biodegradation mechanisms have been suggested, including cleavage of piperazine ring, substitution of F atom, hydroxylation, decarboxylation, and acetylation, as the main biotransformation reactions. This study discovers the ability of non-reported bacterial strains to biodegrade both CIP and LEV as a sole carbon source, providing new insights into the biodegradation of CIP and LEV. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11274-022-03367-5. Springer Netherlands 2022-08-16 2022 /pmc/articles/PMC9381475/ /pubmed/35972564 http://dx.doi.org/10.1007/s11274-022-03367-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Shaker, Reham Alaa Eldin Nagy, Yosra Ibrahim Adly, Mina E. Khattab, Rania Abdelmonem Ragab, Yasser M. Acinetobacter baumannii, Klebsiella pneumoniae and Elizabethkingia miricola isolated from wastewater have biodegradable activity against fluoroquinolone |
title | Acinetobacter baumannii, Klebsiella pneumoniae and Elizabethkingia miricola isolated from wastewater have biodegradable activity against fluoroquinolone |
title_full | Acinetobacter baumannii, Klebsiella pneumoniae and Elizabethkingia miricola isolated from wastewater have biodegradable activity against fluoroquinolone |
title_fullStr | Acinetobacter baumannii, Klebsiella pneumoniae and Elizabethkingia miricola isolated from wastewater have biodegradable activity against fluoroquinolone |
title_full_unstemmed | Acinetobacter baumannii, Klebsiella pneumoniae and Elizabethkingia miricola isolated from wastewater have biodegradable activity against fluoroquinolone |
title_short | Acinetobacter baumannii, Klebsiella pneumoniae and Elizabethkingia miricola isolated from wastewater have biodegradable activity against fluoroquinolone |
title_sort | acinetobacter baumannii, klebsiella pneumoniae and elizabethkingia miricola isolated from wastewater have biodegradable activity against fluoroquinolone |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9381475/ https://www.ncbi.nlm.nih.gov/pubmed/35972564 http://dx.doi.org/10.1007/s11274-022-03367-5 |
work_keys_str_mv | AT shakerrehamalaaeldin acinetobacterbaumanniiklebsiellapneumoniaeandelizabethkingiamiricolaisolatedfromwastewaterhavebiodegradableactivityagainstfluoroquinolone AT nagyyosraibrahim acinetobacterbaumanniiklebsiellapneumoniaeandelizabethkingiamiricolaisolatedfromwastewaterhavebiodegradableactivityagainstfluoroquinolone AT adlyminae acinetobacterbaumanniiklebsiellapneumoniaeandelizabethkingiamiricolaisolatedfromwastewaterhavebiodegradableactivityagainstfluoroquinolone AT khattabraniaabdelmonem acinetobacterbaumanniiklebsiellapneumoniaeandelizabethkingiamiricolaisolatedfromwastewaterhavebiodegradableactivityagainstfluoroquinolone AT ragabyasserm acinetobacterbaumanniiklebsiellapneumoniaeandelizabethkingiamiricolaisolatedfromwastewaterhavebiodegradableactivityagainstfluoroquinolone |