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Insights into Ag-NPs-mediated pathophysiology and ultrastructural aberrations in ovarian tissues of darkling beetles

With the evolution of nanostructure materials, silver nanoparticles (Ag-NPs) emerged as the predominantly exploited nanomaterial in multifarious sectors due to their versatile properties. Along with the heightening applications of Ag-NPs, however, there is increasing concern over their indubitable t...

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Autores principales: El-Samad, Lamia M., Hassan, Mohamed A., Bakr, Nahed R., El-Ashram, Saeed, Radwan, Eman H., Abdul Aziz, Karoline K., Hussein, Hussein K., El Wakil, Abeer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9381597/
https://www.ncbi.nlm.nih.gov/pubmed/35974115
http://dx.doi.org/10.1038/s41598-022-17712-z
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author El-Samad, Lamia M.
Hassan, Mohamed A.
Bakr, Nahed R.
El-Ashram, Saeed
Radwan, Eman H.
Abdul Aziz, Karoline K.
Hussein, Hussein K.
El Wakil, Abeer
author_facet El-Samad, Lamia M.
Hassan, Mohamed A.
Bakr, Nahed R.
El-Ashram, Saeed
Radwan, Eman H.
Abdul Aziz, Karoline K.
Hussein, Hussein K.
El Wakil, Abeer
author_sort El-Samad, Lamia M.
collection PubMed
description With the evolution of nanostructure materials, silver nanoparticles (Ag-NPs) emerged as the predominantly exploited nanomaterial in multifarious sectors due to their versatile properties. Along with the heightening applications of Ag-NPs, however, there is increasing concern over their indubitable toxicity towards the ecosystem, which indeed affects surrounding organisms and human health. In this study, we evaluated the detrimental effects of Ag-NPs in relation to Egyptian wild female beetles, Blaps polychresta, after injection with a single dose of Ag-NPs at different doses and monitoring for 30 days to determine the sublethal dose. Accordingly, the sublethal dose revealed the lowest negative influence was found at 0.03 mg/g body weight. The adverse impacts of Ag-NPs on the ovaries of female beetles were investigated by estimating the enzyme activities, DNA damage using a comet assay, and apoptosis by means of flow cytometry. Besides, the ultrastructural abnormalities were surveyed adopting transmission electron microscopy (TEM). The results manifested comet cells of 7.67 ± 0.88% and 22.33 ± 0.51 for Ag-NPs treated and control groups, respectively. Similarly, the data from flow cytometry demonstrated a substantial reduction in viable cells associated with a significant rise in apoptotic cells for the Ag-NPs treated group in comparison with the control group. Moreover, significant disturbances in enzyme activities for the treated group were perceived correlated with evident diminutions in antioxidant enzymes. Remarkably, the ultrastructural investigation emphasized these findings, exposing considerable deformities of the ovaries in the Ag-NPs treated group compared with the control group. To the best of our knowledge, this is the first report discussing the influence of Ag-NPs at the lowest dose on ovaries of B. polychresta. Collectively, our findings would significantly contribute to considering the critical effects of Ag-NPs at low levels, in addition to the potential use of B. polychresta as a good bio-indicator in ecotoxicological analyses.
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spelling pubmed-93815972022-08-18 Insights into Ag-NPs-mediated pathophysiology and ultrastructural aberrations in ovarian tissues of darkling beetles El-Samad, Lamia M. Hassan, Mohamed A. Bakr, Nahed R. El-Ashram, Saeed Radwan, Eman H. Abdul Aziz, Karoline K. Hussein, Hussein K. El Wakil, Abeer Sci Rep Article With the evolution of nanostructure materials, silver nanoparticles (Ag-NPs) emerged as the predominantly exploited nanomaterial in multifarious sectors due to their versatile properties. Along with the heightening applications of Ag-NPs, however, there is increasing concern over their indubitable toxicity towards the ecosystem, which indeed affects surrounding organisms and human health. In this study, we evaluated the detrimental effects of Ag-NPs in relation to Egyptian wild female beetles, Blaps polychresta, after injection with a single dose of Ag-NPs at different doses and monitoring for 30 days to determine the sublethal dose. Accordingly, the sublethal dose revealed the lowest negative influence was found at 0.03 mg/g body weight. The adverse impacts of Ag-NPs on the ovaries of female beetles were investigated by estimating the enzyme activities, DNA damage using a comet assay, and apoptosis by means of flow cytometry. Besides, the ultrastructural abnormalities were surveyed adopting transmission electron microscopy (TEM). The results manifested comet cells of 7.67 ± 0.88% and 22.33 ± 0.51 for Ag-NPs treated and control groups, respectively. Similarly, the data from flow cytometry demonstrated a substantial reduction in viable cells associated with a significant rise in apoptotic cells for the Ag-NPs treated group in comparison with the control group. Moreover, significant disturbances in enzyme activities for the treated group were perceived correlated with evident diminutions in antioxidant enzymes. Remarkably, the ultrastructural investigation emphasized these findings, exposing considerable deformities of the ovaries in the Ag-NPs treated group compared with the control group. To the best of our knowledge, this is the first report discussing the influence of Ag-NPs at the lowest dose on ovaries of B. polychresta. Collectively, our findings would significantly contribute to considering the critical effects of Ag-NPs at low levels, in addition to the potential use of B. polychresta as a good bio-indicator in ecotoxicological analyses. Nature Publishing Group UK 2022-08-16 /pmc/articles/PMC9381597/ /pubmed/35974115 http://dx.doi.org/10.1038/s41598-022-17712-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
El-Samad, Lamia M.
Hassan, Mohamed A.
Bakr, Nahed R.
El-Ashram, Saeed
Radwan, Eman H.
Abdul Aziz, Karoline K.
Hussein, Hussein K.
El Wakil, Abeer
Insights into Ag-NPs-mediated pathophysiology and ultrastructural aberrations in ovarian tissues of darkling beetles
title Insights into Ag-NPs-mediated pathophysiology and ultrastructural aberrations in ovarian tissues of darkling beetles
title_full Insights into Ag-NPs-mediated pathophysiology and ultrastructural aberrations in ovarian tissues of darkling beetles
title_fullStr Insights into Ag-NPs-mediated pathophysiology and ultrastructural aberrations in ovarian tissues of darkling beetles
title_full_unstemmed Insights into Ag-NPs-mediated pathophysiology and ultrastructural aberrations in ovarian tissues of darkling beetles
title_short Insights into Ag-NPs-mediated pathophysiology and ultrastructural aberrations in ovarian tissues of darkling beetles
title_sort insights into ag-nps-mediated pathophysiology and ultrastructural aberrations in ovarian tissues of darkling beetles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9381597/
https://www.ncbi.nlm.nih.gov/pubmed/35974115
http://dx.doi.org/10.1038/s41598-022-17712-z
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