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Combinatorial therapeutic approaches of photodynamic therapy and immune checkpoint blockade for colon cancer treatment

Photodynamic therapy (PDT) has shown impressive therapeutic effects on various types of cancers by reactive oxygen species (ROS) generation and induction of immune responses. However, under certain conditions, the immune responses induced by PDT are not always sufficient to eradicate the remaining t...

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Autores principales: Hao, Yang, Chung, Chih Kit, Gu, Zili, Schomann, Timo, Dong, Xiaoxu, Veld, Ruben V. Huis in ‘t, Camps, Marcel G. M., ten Dijke, Peter, Ossendorp, Ferry A., Cruz, Luis J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Nature Singapore 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9381671/
https://www.ncbi.nlm.nih.gov/pubmed/35974207
http://dx.doi.org/10.1186/s43556-022-00086-z
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author Hao, Yang
Chung, Chih Kit
Gu, Zili
Schomann, Timo
Dong, Xiaoxu
Veld, Ruben V. Huis in ‘t
Camps, Marcel G. M.
ten Dijke, Peter
Ossendorp, Ferry A.
Cruz, Luis J.
author_facet Hao, Yang
Chung, Chih Kit
Gu, Zili
Schomann, Timo
Dong, Xiaoxu
Veld, Ruben V. Huis in ‘t
Camps, Marcel G. M.
ten Dijke, Peter
Ossendorp, Ferry A.
Cruz, Luis J.
author_sort Hao, Yang
collection PubMed
description Photodynamic therapy (PDT) has shown impressive therapeutic effects on various types of cancers by reactive oxygen species (ROS) generation and induction of immune responses. However, under certain conditions, the immune responses induced by PDT are not always sufficient to eradicate the remaining tumor cells. On the other hand, the photosensitizer indocyanine green (ICG) can mediate PDT under near-infrared (NIR) illumination, thereby enhancing the penetration depth of the excitation light into the tumor. We found that ICG is rapidly taken up in vitro by colorectal MC38 and CT26 tumor cells and it promotes PDT-mediated cell-killing effects. Our results furthermore revealed that ICG induces immunogenic cell death (ICD), as dendritic cells (DCs) were found to engulf ICG-PDT-treated tumor cells and undergo phenotypic maturation. ICG accumulated in tumors 2 h after administration, as measured by fluorescence and photoacoustic imaging. Considering the advantages of ICG as a photosensitizer, we sought to design a therapy that combines PDT and immune checkpoint blockade to maximize tumor control. To this end, a 25% thermosensitive polymer 407 hydrogel was included as a co-delivery platform for this treatment scheme. NIR-PDT under 808 nm irradiation in combination with cytotoxic T-lymphocyte-associated protein 4 (CTLA4)/programmed death-ligand 1 (PD-L1) checkpoint blockade prolonged survival rate of colorectal tumor-bearing mice by inducing a series of immune responses, like the phagocytosis of tumor debris by macrophages and DCs, and induction of acute inflammation, leukocyte infiltration, maturation and activation of DCs. Altogether, our work presents a NIR-triggered PDT strategy in combination with immune checkpoint blockade. Compared to a single treatment, the combination treatment increased efficiency to inhibit solid tumor growth and improved the survival rate of tumor-bearing mice. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43556-022-00086-z.
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spelling pubmed-93816712022-08-18 Combinatorial therapeutic approaches of photodynamic therapy and immune checkpoint blockade for colon cancer treatment Hao, Yang Chung, Chih Kit Gu, Zili Schomann, Timo Dong, Xiaoxu Veld, Ruben V. Huis in ‘t Camps, Marcel G. M. ten Dijke, Peter Ossendorp, Ferry A. Cruz, Luis J. Mol Biomed Research Photodynamic therapy (PDT) has shown impressive therapeutic effects on various types of cancers by reactive oxygen species (ROS) generation and induction of immune responses. However, under certain conditions, the immune responses induced by PDT are not always sufficient to eradicate the remaining tumor cells. On the other hand, the photosensitizer indocyanine green (ICG) can mediate PDT under near-infrared (NIR) illumination, thereby enhancing the penetration depth of the excitation light into the tumor. We found that ICG is rapidly taken up in vitro by colorectal MC38 and CT26 tumor cells and it promotes PDT-mediated cell-killing effects. Our results furthermore revealed that ICG induces immunogenic cell death (ICD), as dendritic cells (DCs) were found to engulf ICG-PDT-treated tumor cells and undergo phenotypic maturation. ICG accumulated in tumors 2 h after administration, as measured by fluorescence and photoacoustic imaging. Considering the advantages of ICG as a photosensitizer, we sought to design a therapy that combines PDT and immune checkpoint blockade to maximize tumor control. To this end, a 25% thermosensitive polymer 407 hydrogel was included as a co-delivery platform for this treatment scheme. NIR-PDT under 808 nm irradiation in combination with cytotoxic T-lymphocyte-associated protein 4 (CTLA4)/programmed death-ligand 1 (PD-L1) checkpoint blockade prolonged survival rate of colorectal tumor-bearing mice by inducing a series of immune responses, like the phagocytosis of tumor debris by macrophages and DCs, and induction of acute inflammation, leukocyte infiltration, maturation and activation of DCs. Altogether, our work presents a NIR-triggered PDT strategy in combination with immune checkpoint blockade. Compared to a single treatment, the combination treatment increased efficiency to inhibit solid tumor growth and improved the survival rate of tumor-bearing mice. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43556-022-00086-z. Springer Nature Singapore 2022-08-17 /pmc/articles/PMC9381671/ /pubmed/35974207 http://dx.doi.org/10.1186/s43556-022-00086-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Hao, Yang
Chung, Chih Kit
Gu, Zili
Schomann, Timo
Dong, Xiaoxu
Veld, Ruben V. Huis in ‘t
Camps, Marcel G. M.
ten Dijke, Peter
Ossendorp, Ferry A.
Cruz, Luis J.
Combinatorial therapeutic approaches of photodynamic therapy and immune checkpoint blockade for colon cancer treatment
title Combinatorial therapeutic approaches of photodynamic therapy and immune checkpoint blockade for colon cancer treatment
title_full Combinatorial therapeutic approaches of photodynamic therapy and immune checkpoint blockade for colon cancer treatment
title_fullStr Combinatorial therapeutic approaches of photodynamic therapy and immune checkpoint blockade for colon cancer treatment
title_full_unstemmed Combinatorial therapeutic approaches of photodynamic therapy and immune checkpoint blockade for colon cancer treatment
title_short Combinatorial therapeutic approaches of photodynamic therapy and immune checkpoint blockade for colon cancer treatment
title_sort combinatorial therapeutic approaches of photodynamic therapy and immune checkpoint blockade for colon cancer treatment
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9381671/
https://www.ncbi.nlm.nih.gov/pubmed/35974207
http://dx.doi.org/10.1186/s43556-022-00086-z
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