Cargando…
New conductive filament ready-to-use for 3D-printing electrochemical (bio)sensors: Towards the detection of SARS-CoV-2
The 3D printing technology has gained ground due to its wide range of applicability. The development of new conductive filaments contributes significantly to the production of improved electrochemical devices. In this context, we report a simple method to producing an efficient conductive filament,...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9381826/ https://www.ncbi.nlm.nih.gov/pubmed/35033268 http://dx.doi.org/10.1016/j.aca.2021.339372 |
_version_ | 1784769160138457088 |
---|---|
author | Stefano, Jéssica Santos Guterres e Silva, Luiz Ricardo Rocha, Raquel Gomes Brazaca, Laís Canniatti Richter, Eduardo Mathias Abarza Muñoz, Rodrigo Alejandro Janegitz, Bruno Campos |
author_facet | Stefano, Jéssica Santos Guterres e Silva, Luiz Ricardo Rocha, Raquel Gomes Brazaca, Laís Canniatti Richter, Eduardo Mathias Abarza Muñoz, Rodrigo Alejandro Janegitz, Bruno Campos |
author_sort | Stefano, Jéssica Santos |
collection | PubMed |
description | The 3D printing technology has gained ground due to its wide range of applicability. The development of new conductive filaments contributes significantly to the production of improved electrochemical devices. In this context, we report a simple method to producing an efficient conductive filament, containing graphite within the polymer matrix of PLA, and applied in conjunction with 3D printing technology to generate (bio)sensors without the need for surface activation. The proposed method for producing the conductive filament consists of four steps: (i) mixing graphite and PLA in a heated reflux system; (ii) recrystallization of the composite; (iii) drying and; (iv) extrusion. The produced filament was used for the manufacture of electrochemical 3D printed sensors. The filament and sensor were characterized by physicochemical techniques, such as SEM, TGA, Raman, FTIR as well as electrochemical techniques (EIS and CV). Finally, as a proof-of-concept, the fabricated 3D-printed sensor was applied for the determination of uric acid and dopamine in synthetic urine and used as a platform for the development of a biosensor for the detection of SARS-CoV-2. The developed sensors, without pre-treatment, provided linear ranges of 0.5–150.0 and 5.0–50.0 μmol L(−1), with low LOD values (0.07 and 0.11 μmol L(−1)), for uric acid and dopamine, respectively. The developed biosensor successfully detected SARS-CoV-2 S protein, with a linear range from 5.0 to 75.0 nmol L(−1) (0.38 μg mL(−1) to 5.74 μg mL(−1)) and LOD of 1.36 nmol L(−1) (0.10 μg mL(−1)) and sensitivity of 0.17 μA nmol(−1) L (0.01 μA μg(−1) mL). Therefore, the lab-made produced and the ready-to-use conductive filament is promising and can become an alternative route for the production of different 3D electrochemical (bio)sensors and other types of conductive devices by 3D printing. |
format | Online Article Text |
id | pubmed-9381826 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93818262022-08-17 New conductive filament ready-to-use for 3D-printing electrochemical (bio)sensors: Towards the detection of SARS-CoV-2 Stefano, Jéssica Santos Guterres e Silva, Luiz Ricardo Rocha, Raquel Gomes Brazaca, Laís Canniatti Richter, Eduardo Mathias Abarza Muñoz, Rodrigo Alejandro Janegitz, Bruno Campos Anal Chim Acta Article The 3D printing technology has gained ground due to its wide range of applicability. The development of new conductive filaments contributes significantly to the production of improved electrochemical devices. In this context, we report a simple method to producing an efficient conductive filament, containing graphite within the polymer matrix of PLA, and applied in conjunction with 3D printing technology to generate (bio)sensors without the need for surface activation. The proposed method for producing the conductive filament consists of four steps: (i) mixing graphite and PLA in a heated reflux system; (ii) recrystallization of the composite; (iii) drying and; (iv) extrusion. The produced filament was used for the manufacture of electrochemical 3D printed sensors. The filament and sensor were characterized by physicochemical techniques, such as SEM, TGA, Raman, FTIR as well as electrochemical techniques (EIS and CV). Finally, as a proof-of-concept, the fabricated 3D-printed sensor was applied for the determination of uric acid and dopamine in synthetic urine and used as a platform for the development of a biosensor for the detection of SARS-CoV-2. The developed sensors, without pre-treatment, provided linear ranges of 0.5–150.0 and 5.0–50.0 μmol L(−1), with low LOD values (0.07 and 0.11 μmol L(−1)), for uric acid and dopamine, respectively. The developed biosensor successfully detected SARS-CoV-2 S protein, with a linear range from 5.0 to 75.0 nmol L(−1) (0.38 μg mL(−1) to 5.74 μg mL(−1)) and LOD of 1.36 nmol L(−1) (0.10 μg mL(−1)) and sensitivity of 0.17 μA nmol(−1) L (0.01 μA μg(−1) mL). Therefore, the lab-made produced and the ready-to-use conductive filament is promising and can become an alternative route for the production of different 3D electrochemical (bio)sensors and other types of conductive devices by 3D printing. Elsevier B.V. 2022-01-25 2021-12-11 /pmc/articles/PMC9381826/ /pubmed/35033268 http://dx.doi.org/10.1016/j.aca.2021.339372 Text en © 2021 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Stefano, Jéssica Santos Guterres e Silva, Luiz Ricardo Rocha, Raquel Gomes Brazaca, Laís Canniatti Richter, Eduardo Mathias Abarza Muñoz, Rodrigo Alejandro Janegitz, Bruno Campos New conductive filament ready-to-use for 3D-printing electrochemical (bio)sensors: Towards the detection of SARS-CoV-2 |
title | New conductive filament ready-to-use for 3D-printing electrochemical (bio)sensors: Towards the detection of SARS-CoV-2 |
title_full | New conductive filament ready-to-use for 3D-printing electrochemical (bio)sensors: Towards the detection of SARS-CoV-2 |
title_fullStr | New conductive filament ready-to-use for 3D-printing electrochemical (bio)sensors: Towards the detection of SARS-CoV-2 |
title_full_unstemmed | New conductive filament ready-to-use for 3D-printing electrochemical (bio)sensors: Towards the detection of SARS-CoV-2 |
title_short | New conductive filament ready-to-use for 3D-printing electrochemical (bio)sensors: Towards the detection of SARS-CoV-2 |
title_sort | new conductive filament ready-to-use for 3d-printing electrochemical (bio)sensors: towards the detection of sars-cov-2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9381826/ https://www.ncbi.nlm.nih.gov/pubmed/35033268 http://dx.doi.org/10.1016/j.aca.2021.339372 |
work_keys_str_mv | AT stefanojessicasantos newconductivefilamentreadytousefor3dprintingelectrochemicalbiosensorstowardsthedetectionofsarscov2 AT guterresesilvaluizricardo newconductivefilamentreadytousefor3dprintingelectrochemicalbiosensorstowardsthedetectionofsarscov2 AT rocharaquelgomes newconductivefilamentreadytousefor3dprintingelectrochemicalbiosensorstowardsthedetectionofsarscov2 AT brazacalaiscanniatti newconductivefilamentreadytousefor3dprintingelectrochemicalbiosensorstowardsthedetectionofsarscov2 AT richtereduardomathias newconductivefilamentreadytousefor3dprintingelectrochemicalbiosensorstowardsthedetectionofsarscov2 AT abarzamunozrodrigoalejandro newconductivefilamentreadytousefor3dprintingelectrochemicalbiosensorstowardsthedetectionofsarscov2 AT janegitzbrunocampos newconductivefilamentreadytousefor3dprintingelectrochemicalbiosensorstowardsthedetectionofsarscov2 |