Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system

In this protocol, we describe how to generate 3D culture surrogates of chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) bone marrow microenvironments. We detail the use of culturing scaffolds populated with BM stromal cells and tumor cells in the RCCS™ bioreactor. This 3D culture can eff...

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Autores principales: Belloni, Daniela, Ferrarini, Marina, Ferrero, Elisabetta, Guzzeloni, Virginia, Barbaglio, Federica, Ghia, Paolo, Scielzo, Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9382330/
https://www.ncbi.nlm.nih.gov/pubmed/35990738
http://dx.doi.org/10.1016/j.xpro.2022.101601
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author Belloni, Daniela
Ferrarini, Marina
Ferrero, Elisabetta
Guzzeloni, Virginia
Barbaglio, Federica
Ghia, Paolo
Scielzo, Cristina
author_facet Belloni, Daniela
Ferrarini, Marina
Ferrero, Elisabetta
Guzzeloni, Virginia
Barbaglio, Federica
Ghia, Paolo
Scielzo, Cristina
author_sort Belloni, Daniela
collection PubMed
description In this protocol, we describe how to generate 3D culture surrogates of chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) bone marrow microenvironments. We detail the use of culturing scaffolds populated with BM stromal cells and tumor cells in the RCCS™ bioreactor. This 3D culture can efficiently recapitulate tumor-stroma crosstalk and allows the testing of drugs such as ibrutinib and bortezomib. Moreover, this protocol can be used for the generation of other and more complex tumor microenvironments. For complete details on the use and execution of this protocol, please refer to Belloni et al. (2018) and Barbaglio et al. (2021).
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spelling pubmed-93823302022-08-18 Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system Belloni, Daniela Ferrarini, Marina Ferrero, Elisabetta Guzzeloni, Virginia Barbaglio, Federica Ghia, Paolo Scielzo, Cristina STAR Protoc Protocol In this protocol, we describe how to generate 3D culture surrogates of chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) bone marrow microenvironments. We detail the use of culturing scaffolds populated with BM stromal cells and tumor cells in the RCCS™ bioreactor. This 3D culture can efficiently recapitulate tumor-stroma crosstalk and allows the testing of drugs such as ibrutinib and bortezomib. Moreover, this protocol can be used for the generation of other and more complex tumor microenvironments. For complete details on the use and execution of this protocol, please refer to Belloni et al. (2018) and Barbaglio et al. (2021). Elsevier 2022-08-10 /pmc/articles/PMC9382330/ /pubmed/35990738 http://dx.doi.org/10.1016/j.xpro.2022.101601 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Belloni, Daniela
Ferrarini, Marina
Ferrero, Elisabetta
Guzzeloni, Virginia
Barbaglio, Federica
Ghia, Paolo
Scielzo, Cristina
Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system
title Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system
title_full Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system
title_fullStr Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system
title_full_unstemmed Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system
title_short Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system
title_sort protocol for generation of 3d bone marrow surrogate microenvironments in a rotary cell culture system
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9382330/
https://www.ncbi.nlm.nih.gov/pubmed/35990738
http://dx.doi.org/10.1016/j.xpro.2022.101601
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