Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system

In this protocol, we describe how to generate 3D culture surrogates of chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) bone marrow microenvironments. We detail the use of culturing scaffolds populated with BM stromal cells and tumor cells in the RCCS™ bioreactor. This 3D culture can eff...

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Detalles Bibliográficos
Autores principales: Belloni, Daniela, Ferrarini, Marina, Ferrero, Elisabetta, Guzzeloni, Virginia, Barbaglio, Federica, Ghia, Paolo, Scielzo, Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9382330/
https://www.ncbi.nlm.nih.gov/pubmed/35990738
http://dx.doi.org/10.1016/j.xpro.2022.101601

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9382330/
https://www.ncbi.nlm.nih.gov/pubmed/35990738
http://dx.doi.org/10.1016/j.xpro.2022.101601

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