Cargando…

Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system

In this protocol, we describe how to generate 3D culture surrogates of chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) bone marrow microenvironments. We detail the use of culturing scaffolds populated with BM stromal cells and tumor cells in the RCCS™ bioreactor. This 3D culture can eff...

Descripción completa

Detalles Bibliográficos
Autores principales:	Belloni, Daniela, Ferrarini, Marina, Ferrero, Elisabetta, Guzzeloni, Virginia, Barbaglio, Federica, Ghia, Paolo, Scielzo, Cristina
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9382330/ https://www.ncbi.nlm.nih.gov/pubmed/35990738 http://dx.doi.org/10.1016/j.xpro.2022.101601

Ejemplares similares

Three-dimensional co-culture model of chronic lymphocytic leukemia bone marrow microenvironment predicts patient-specific response to mobilizing agents
por: Barbaglio, Federica, et al.
Publicado: (2020)

3D Models as a Tool to Assess the Anti-Tumor Efficacy of Therapeutic Antibodies: Advantages and Limitations
por: Guzzeloni, Virginia, et al.
Publicado: (2022)

Modeling multiple myeloma-bone marrow interactions and response to drugs in a 3D surrogate microenvironment
por: Belloni, Daniela, et al.
Publicado: (2018)

PYK2 is overexpressed in chronic lymphocytic leukaemia: A potential new therapeutic target
por: Sbrana, Francesca Vittoria, et al.
Publicado: (2023)

3D Bioprinting Allows the Establishment of Long-Term 3D Culture Model for Chronic Lymphocytic Leukemia Cells
por: Sbrana, Francesca Vittoria, et al.
Publicado: (2021)

Modeling the Leukemia Microenviroment In Vitro
por: Scielzo, Cristina, et al.
Publicado: (2020)

Determining (n,f) and (n,Y) cross sections using surrogate reactions
por: Scielzo, N D
Publicado: (2010)

Ex-Vivo Dynamic 3-D Culture of Human Tissues in the RCCS™ Bioreactor Allows the Study of Multiple Myeloma Biology and Response to Therapy
por: Ferrarini, Marina, et al.
Publicado: (2013)

Correction: Ex-Vivo Dynamic 3-D Culture of Human Tissues in the RCCS™ Bioreactor Allows the Study of Multiple Myeloma Biology and Response to Therapy
por: Ferrarini, Marina, et al.
Publicado: (2013)

Correction: Establishment and Characterization of PCL12, a Novel CD5+ Chronic Lymphocytic Leukaemia Cell Line
por: Agathangelidis, Andreas, et al.
Publicado: (2015)

Establishment and Characterization of PCL12, a Novel CD5(+) Chronic Lymphocytic Leukaemia Cell Line
por: Agathangelidis, Andreas, et al.
Publicado: (2015)

3D culture of Erdheim-Chester disease tissues unveils histiocyte metabolism as a new therapeutic target
por: Villa, Antonello, et al.
Publicado: (2019)

Synthetic high-density lipoproteins as targeted monotherapy for chronic lymphocytic leukemia
por: McMahon, Kaylin M., et al.
Publicado: (2017)

3D-STED Super-Resolution Microscopy Reveals Distinct Nanoscale Organization of the Hematopoietic Cell-Specific Lyn Substrate-1 (HS1) in Normal and Leukemic B Cells
por: Sampietro, Marta, et al.
Publicado: (2021)

Surrogate-based optimization design for surface texture of helical pair in helical hydraulic rotary actuator
por: Liu, Song, et al.
Publicado: (2023)

Preparation and culture of bone marrow-derived macrophages from mice for functional analysis
por: Toda, Gotaro, et al.
Publicado: (2020)

Sonic hedgehog promotes chondrogenesis of rabbit bone marrow stem cells in a rotary cell culture system
por: Chen, Liyang, et al.
Publicado: (2019)

Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
por: Ganan-Gomez, Irene, et al.
Publicado: (2022)

The Nanomechanical Properties of CLL Cells Are Linked to the Actin Cytoskeleton and Are a Potential Target of BTK Inhibitors
por: Sampietro, Marta, et al.
Publicado: (2023)

Production of Clubroot Standards Using a Recombinant Surrogate to Overcome Natural Genetic Variability
por: Patel, Anjana, et al.
Publicado: (2023)

Novel Protocol for the Preparation of Porcine Bone Marrow Primary Cell Culture for African Swine Fever Virus Isolation
por: Puzankova, Olga, et al.
Publicado: (2023)

Emerging Strategies in 3D Culture Models for Hematological Cancers
por: Barozzi, Dafne, et al.
Publicado: (2023)

Efficient Generation of Chondrocytes From Bone Marrow–Derived Mesenchymal Stem Cells in a 3D Culture System: Protocol for a Practical Model for Assessing Anti-Inflammatory Therapies
por: Patnaik, Rajashree, et al.
Publicado: (2023)

Bone Marrow Microenvironment-On-Chip for Culture of Functional Hematopoietic Stem Cells
por: Sharipol, Azmeer, et al.
Publicado: (2022)

Protocol for performing consecutive bone marrow transplants in mice to study the role of marrow niche in supporting hematopoiesis
por: Gopal, Aparna, et al.
Publicado: (2023)

Isolation of human bone marrow stromal cells from bone marrow biopsies for single-cell RNA sequencing
por: Gleitz, Hélène F.E., et al.
Publicado: (2021)

Bone marrow stromal cells generate an osteoinductive microenvironment when cultured on titanium–aluminum–vanadium substrates with biomimetic multiscale surface roughness
por: Berger, Michael B, et al.
Publicado: (2023)

Generation of precision-cut slice cultures of human placenta
por: Fahmi, Amal, et al.
Publicado: (2022)

An Investigation of the Clinical Effects of Lævo-Rotary and Dextro-Rotary Hyoscine
por: Moir, J. Chassar
Publicado: (1925)

The dynamic stator stalk of rotary ATPases
por: Stewart, Alastair G., et al.
Publicado: (2012)

Use of Busulfan to Condition Mice for Bone Marrow Transplantation
por: Montecino-Rodriguez, Encarnacion, et al.
Publicado: (2020)

ATR addiction in multiple myeloma: synthetic lethal approaches exploiting established therapies
por: Botrugno, Oronza A., et al.
Publicado: (2019)

Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system
por: Chen, Liyang, et al.
Publicado: (2019)

Generation of Vascularized Neural Organoids by Co-culturing with Mesodermal Progenitor Cells
por: Wörsdörfer, Philipp, et al.
Publicado: (2020)

Generation, culture, and stimulation of small intestinal murine organoids in parasitology research
por: Campillo Poveda, Marta, et al.
Publicado: (2023)

A protocol for human bone marrow adipocyte isolation and purification
por: Attané, Camille, et al.
Publicado: (2021)

Mass cytometry staining for human bone marrow clinical samples
por: Hallisey, Margaret, et al.
Publicado: (2022)

The Bone Marrow Microenvironment as Niche Retreats for Hematopoietic and Leukemic Stem Cells
por: Nwajei, Felix, et al.
Publicado: (2013)

Intratumoral Monocyte Transfer to Examine Monocyte Differentiation in the Tumor Microenvironment
por: Devalaraja, Samir, et al.
Publicado: (2020)

Murine brain tumor microenvironment immunophenotyping using mass cytometry
por: McClellan, Brandon L., et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_65hvjhajfj8rg5l69h2abcjhto