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Molecular detection of pathogenic bacteria in the colonic biopsies from patients with Ulcerative Colitis

BACKGROUND/AIM: Ulcerative Colitis (UC) is an inflammatory bowel disease which is common in many areas of the world including Egypt. A lot of controversy regarding the pathogenesis of UC exist. The current study is an attempt to detect some pathogenic bacteria in UC patients. MATERIALS AND METHODS:...

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Autores principales: El A Helal, Thanaa, El Abdel Wahab, Hoda E, Saber, Sally M, Abdelaaty, Waleed H, Eltabbakh, Mohamed M, Aref, Ahmed M, Dawood, Mohamed H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Makerere Medical School 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9382498/
https://www.ncbi.nlm.nih.gov/pubmed/36032461
http://dx.doi.org/10.4314/ahs.v22i1.70
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author El A Helal, Thanaa
El Abdel Wahab, Hoda E
Saber, Sally M
Abdelaaty, Waleed H
Eltabbakh, Mohamed M
Aref, Ahmed M
Dawood, Mohamed H
author_facet El A Helal, Thanaa
El Abdel Wahab, Hoda E
Saber, Sally M
Abdelaaty, Waleed H
Eltabbakh, Mohamed M
Aref, Ahmed M
Dawood, Mohamed H
author_sort El A Helal, Thanaa
collection PubMed
description BACKGROUND/AIM: Ulcerative Colitis (UC) is an inflammatory bowel disease which is common in many areas of the world including Egypt. A lot of controversy regarding the pathogenesis of UC exist. The current study is an attempt to detect some pathogenic bacteria in UC patients. MATERIALS AND METHODS: Endoscopic colonic biopsies obtained from 40 patients with ulcerative colitis and 20 controls were analyzed by means of real-time PCR technique for the presence of Clostridium difficile, Helicobacter Pylori (H. pylori) and pathogenic Escherichia Coli (E. coli) which are positive for KPC and/or OXA-48. RESULTS: All patients and control samples were negative for Clostridium difficile. Three of the 40 patient samples (7.5%) and none of the 20 controls were positive for H. pylori with no significant difference between the two groups. KPC-positive E. coli were detected in 11 of the 40 patients (27.5%) and in none of the controls with a significant difference between the two groups (P=0.01). All patients and control samples were negative for OXA-48 positive E. coli. CONCLUSION: Although this study does not support the claim that Clostridium difficile and/or H. pylori have a role in UC, it greatly suggests that pathogenic E. coli may be involved in one way or another in the course of UC.
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spelling pubmed-93824982022-08-25 Molecular detection of pathogenic bacteria in the colonic biopsies from patients with Ulcerative Colitis El A Helal, Thanaa El Abdel Wahab, Hoda E Saber, Sally M Abdelaaty, Waleed H Eltabbakh, Mohamed M Aref, Ahmed M Dawood, Mohamed H Afr Health Sci Articles BACKGROUND/AIM: Ulcerative Colitis (UC) is an inflammatory bowel disease which is common in many areas of the world including Egypt. A lot of controversy regarding the pathogenesis of UC exist. The current study is an attempt to detect some pathogenic bacteria in UC patients. MATERIALS AND METHODS: Endoscopic colonic biopsies obtained from 40 patients with ulcerative colitis and 20 controls were analyzed by means of real-time PCR technique for the presence of Clostridium difficile, Helicobacter Pylori (H. pylori) and pathogenic Escherichia Coli (E. coli) which are positive for KPC and/or OXA-48. RESULTS: All patients and control samples were negative for Clostridium difficile. Three of the 40 patient samples (7.5%) and none of the 20 controls were positive for H. pylori with no significant difference between the two groups. KPC-positive E. coli were detected in 11 of the 40 patients (27.5%) and in none of the controls with a significant difference between the two groups (P=0.01). All patients and control samples were negative for OXA-48 positive E. coli. CONCLUSION: Although this study does not support the claim that Clostridium difficile and/or H. pylori have a role in UC, it greatly suggests that pathogenic E. coli may be involved in one way or another in the course of UC. Makerere Medical School 2022-03 /pmc/articles/PMC9382498/ /pubmed/36032461 http://dx.doi.org/10.4314/ahs.v22i1.70 Text en © 2022 Helal TE-A et al. https://creativecommons.org/licenses/by/4.0/Licensee African Health Sciences. This is an Open Access article distributed under the terms of the Creative commons Attribution License (https://creativecommons.org/licenses/BY/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
El A Helal, Thanaa
El Abdel Wahab, Hoda E
Saber, Sally M
Abdelaaty, Waleed H
Eltabbakh, Mohamed M
Aref, Ahmed M
Dawood, Mohamed H
Molecular detection of pathogenic bacteria in the colonic biopsies from patients with Ulcerative Colitis
title Molecular detection of pathogenic bacteria in the colonic biopsies from patients with Ulcerative Colitis
title_full Molecular detection of pathogenic bacteria in the colonic biopsies from patients with Ulcerative Colitis
title_fullStr Molecular detection of pathogenic bacteria in the colonic biopsies from patients with Ulcerative Colitis
title_full_unstemmed Molecular detection of pathogenic bacteria in the colonic biopsies from patients with Ulcerative Colitis
title_short Molecular detection of pathogenic bacteria in the colonic biopsies from patients with Ulcerative Colitis
title_sort molecular detection of pathogenic bacteria in the colonic biopsies from patients with ulcerative colitis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9382498/
https://www.ncbi.nlm.nih.gov/pubmed/36032461
http://dx.doi.org/10.4314/ahs.v22i1.70
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