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Expression of TRAF6 in peripheral blood B cells of patients with myasthenia gravis
BACKGROUND: Tumor necrosis factor receptor-associated factor 6 (TRAF6) can regulate the activation of inflammatory signaling pathways by acting as an E3 ubiquitin ligase, which enhances B cell activation. This study aimed to evaluate the expression of TRAF6 in the peripheral blood B cells of myasthe...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9382794/ https://www.ncbi.nlm.nih.gov/pubmed/35978310 http://dx.doi.org/10.1186/s12883-022-02833-9 |
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author | Li, Ting Li, Yue Li, Jia-Wen Qin, Ying-Hui Zhai, Hui Feng, Bin Li, He Zhang, Ning-Nannan Yang, Chun-Sheng |
author_facet | Li, Ting Li, Yue Li, Jia-Wen Qin, Ying-Hui Zhai, Hui Feng, Bin Li, He Zhang, Ning-Nannan Yang, Chun-Sheng |
author_sort | Li, Ting |
collection | PubMed |
description | BACKGROUND: Tumor necrosis factor receptor-associated factor 6 (TRAF6) can regulate the activation of inflammatory signaling pathways by acting as an E3 ubiquitin ligase, which enhances B cell activation. This study aimed to evaluate the expression of TRAF6 in the peripheral blood B cells of myasthenia gravis (MG) patients and analyze the relationships between TRAF6 expression and clinical characteristics. METHOD: In our study, the expression level of TRAF6 in peripheral blood B cells of 89 patients was measured by flow cytometry compared with that of healthy subjects. The effects of disease severity, MG classification and immunotherapy on TRAF6 expression level were also analyzed. RESULTS: In our study, TRAF6 expression was elevated in CD19(+) B cells and CD19(+)CD27(+) memory B cells in generalized MG (GMG) patients compared with ocular MG (OMG) patients (p = 0.03 and p = 0.03, respectively). There was a significant positive correlation between the TRAF6 expression level and disease severity in both OMG patients and GMG patients (CD19(+) B cells: OMG: p < 0.001, r = 0.89; GMG: p = 0.001, r = 0.59; CD29(+)CD27(+) B cells: OMG: p = 0.001, r = 0.80; GMG: p = 0.048, r = 0.38). TRAF6 expression was significantly elevated in CD19(+) B cells and CD19(+)CD27(+) memory B cells in GMG with acute aggravation compared with GMG in MMS (p = 0.009 and p = 0.028, respectively). In the eleven MG patients who were followed, TRAF6 expression in B cells and memory B cells was significantly decreased after treatment (p = 0.03 and p < 0.01, respectively). CONCLUSION: TRAF6 is potentially a useful biomarker of inflammation in patients with MG, and might be used to evaluate the effectiveness of treatment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12883-022-02833-9. |
format | Online Article Text |
id | pubmed-9382794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-93827942022-08-18 Expression of TRAF6 in peripheral blood B cells of patients with myasthenia gravis Li, Ting Li, Yue Li, Jia-Wen Qin, Ying-Hui Zhai, Hui Feng, Bin Li, He Zhang, Ning-Nannan Yang, Chun-Sheng BMC Neurol Research BACKGROUND: Tumor necrosis factor receptor-associated factor 6 (TRAF6) can regulate the activation of inflammatory signaling pathways by acting as an E3 ubiquitin ligase, which enhances B cell activation. This study aimed to evaluate the expression of TRAF6 in the peripheral blood B cells of myasthenia gravis (MG) patients and analyze the relationships between TRAF6 expression and clinical characteristics. METHOD: In our study, the expression level of TRAF6 in peripheral blood B cells of 89 patients was measured by flow cytometry compared with that of healthy subjects. The effects of disease severity, MG classification and immunotherapy on TRAF6 expression level were also analyzed. RESULTS: In our study, TRAF6 expression was elevated in CD19(+) B cells and CD19(+)CD27(+) memory B cells in generalized MG (GMG) patients compared with ocular MG (OMG) patients (p = 0.03 and p = 0.03, respectively). There was a significant positive correlation between the TRAF6 expression level and disease severity in both OMG patients and GMG patients (CD19(+) B cells: OMG: p < 0.001, r = 0.89; GMG: p = 0.001, r = 0.59; CD29(+)CD27(+) B cells: OMG: p = 0.001, r = 0.80; GMG: p = 0.048, r = 0.38). TRAF6 expression was significantly elevated in CD19(+) B cells and CD19(+)CD27(+) memory B cells in GMG with acute aggravation compared with GMG in MMS (p = 0.009 and p = 0.028, respectively). In the eleven MG patients who were followed, TRAF6 expression in B cells and memory B cells was significantly decreased after treatment (p = 0.03 and p < 0.01, respectively). CONCLUSION: TRAF6 is potentially a useful biomarker of inflammation in patients with MG, and might be used to evaluate the effectiveness of treatment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12883-022-02833-9. BioMed Central 2022-08-17 /pmc/articles/PMC9382794/ /pubmed/35978310 http://dx.doi.org/10.1186/s12883-022-02833-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Li, Ting Li, Yue Li, Jia-Wen Qin, Ying-Hui Zhai, Hui Feng, Bin Li, He Zhang, Ning-Nannan Yang, Chun-Sheng Expression of TRAF6 in peripheral blood B cells of patients with myasthenia gravis |
title | Expression of TRAF6 in peripheral blood B cells of patients with myasthenia gravis |
title_full | Expression of TRAF6 in peripheral blood B cells of patients with myasthenia gravis |
title_fullStr | Expression of TRAF6 in peripheral blood B cells of patients with myasthenia gravis |
title_full_unstemmed | Expression of TRAF6 in peripheral blood B cells of patients with myasthenia gravis |
title_short | Expression of TRAF6 in peripheral blood B cells of patients with myasthenia gravis |
title_sort | expression of traf6 in peripheral blood b cells of patients with myasthenia gravis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9382794/ https://www.ncbi.nlm.nih.gov/pubmed/35978310 http://dx.doi.org/10.1186/s12883-022-02833-9 |
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