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Development of a mouse salivary gland-derived mesenchymal cell line for immunological studies of murine cytomegalovirus

The salivary glands are a crucial site of replication for human cytomegalovirus (HCMV) and its murine counterpart, murine cytomegalovirus (MCMV). Studies of MCMV often involve the use of BALB/c strain mice, but most in vitro assays are carried out in the NIH 3T3 cell line, which is derived from Swis...

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Autores principales: White, Timothy M., Stanfield, Brent A., Bonavita, Cassandra M., Rudd, Jared S., Cardin, Rhonda D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385033/
https://www.ncbi.nlm.nih.gov/pubmed/35976883
http://dx.doi.org/10.1371/journal.pone.0265479
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author White, Timothy M.
Stanfield, Brent A.
Bonavita, Cassandra M.
Rudd, Jared S.
Cardin, Rhonda D.
author_facet White, Timothy M.
Stanfield, Brent A.
Bonavita, Cassandra M.
Rudd, Jared S.
Cardin, Rhonda D.
author_sort White, Timothy M.
collection PubMed
description The salivary glands are a crucial site of replication for human cytomegalovirus (HCMV) and its murine counterpart, murine cytomegalovirus (MCMV). Studies of MCMV often involve the use of BALB/c strain mice, but most in vitro assays are carried out in the NIH 3T3 cell line, which is derived from Swiss Albino mice. This report describes a BALB/c-derived mouse salivary gland cell line immortalized using the SV40 large T antigen. Cells stained positive for PDGFR1 and negative for E-cadherin and PECAM-1, indicating mesenchymal origin. This cell line, which has been named murine salivary gland mesenchymal (mSGM), shows promise as a tool for ex vivo immunological assays due to its MHC haplotype match with the BALB/c mouse strain. In addition, plaque assays using mSGM rather than NIH 3T3 cells are significantly more sensitive for detecting low concentrations of MCMV particles. Finally, it is demonstrated that mSGM cells express all 3 BALB/c MHC class I isotypes and are susceptible to T cell-mediated ex vivo cytotoxicity assays, leading to many possible uses in immunological studies of MCMV.
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spelling pubmed-93850332022-08-18 Development of a mouse salivary gland-derived mesenchymal cell line for immunological studies of murine cytomegalovirus White, Timothy M. Stanfield, Brent A. Bonavita, Cassandra M. Rudd, Jared S. Cardin, Rhonda D. PLoS One Research Article The salivary glands are a crucial site of replication for human cytomegalovirus (HCMV) and its murine counterpart, murine cytomegalovirus (MCMV). Studies of MCMV often involve the use of BALB/c strain mice, but most in vitro assays are carried out in the NIH 3T3 cell line, which is derived from Swiss Albino mice. This report describes a BALB/c-derived mouse salivary gland cell line immortalized using the SV40 large T antigen. Cells stained positive for PDGFR1 and negative for E-cadherin and PECAM-1, indicating mesenchymal origin. This cell line, which has been named murine salivary gland mesenchymal (mSGM), shows promise as a tool for ex vivo immunological assays due to its MHC haplotype match with the BALB/c mouse strain. In addition, plaque assays using mSGM rather than NIH 3T3 cells are significantly more sensitive for detecting low concentrations of MCMV particles. Finally, it is demonstrated that mSGM cells express all 3 BALB/c MHC class I isotypes and are susceptible to T cell-mediated ex vivo cytotoxicity assays, leading to many possible uses in immunological studies of MCMV. Public Library of Science 2022-08-17 /pmc/articles/PMC9385033/ /pubmed/35976883 http://dx.doi.org/10.1371/journal.pone.0265479 Text en © 2022 White et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
White, Timothy M.
Stanfield, Brent A.
Bonavita, Cassandra M.
Rudd, Jared S.
Cardin, Rhonda D.
Development of a mouse salivary gland-derived mesenchymal cell line for immunological studies of murine cytomegalovirus
title Development of a mouse salivary gland-derived mesenchymal cell line for immunological studies of murine cytomegalovirus
title_full Development of a mouse salivary gland-derived mesenchymal cell line for immunological studies of murine cytomegalovirus
title_fullStr Development of a mouse salivary gland-derived mesenchymal cell line for immunological studies of murine cytomegalovirus
title_full_unstemmed Development of a mouse salivary gland-derived mesenchymal cell line for immunological studies of murine cytomegalovirus
title_short Development of a mouse salivary gland-derived mesenchymal cell line for immunological studies of murine cytomegalovirus
title_sort development of a mouse salivary gland-derived mesenchymal cell line for immunological studies of murine cytomegalovirus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385033/
https://www.ncbi.nlm.nih.gov/pubmed/35976883
http://dx.doi.org/10.1371/journal.pone.0265479
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