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A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2

The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed. On the other hand, several concerning SARS-CoV-2...

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Autores principales: Chen, Zhi, Li, Jingfeng, Li, Tianzhong, Fan, Taojian, Meng, Changle, Li, Chaozhou, Kang, Jianlong, Chai, Luxiao, Hao, Yabin, Tang, Yuxuan, Al-Hartomy, Omar A, Wageh, Swelm, Al-Sehemi, Abdullah G, Luo, Zhiguang, Yu, Jiangtian, Shao, Yonghong, Li, Defa, Feng, Shuai, Liu, William J, He, Yaqing, Ma, Xiaopeng, Xie, Zhongjian, Zhang, Han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385456/
https://www.ncbi.nlm.nih.gov/pubmed/35992231
http://dx.doi.org/10.1093/nsr/nwac104
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author Chen, Zhi
Li, Jingfeng
Li, Tianzhong
Fan, Taojian
Meng, Changle
Li, Chaozhou
Kang, Jianlong
Chai, Luxiao
Hao, Yabin
Tang, Yuxuan
Al-Hartomy, Omar A
Wageh, Swelm
Al-Sehemi, Abdullah G
Luo, Zhiguang
Yu, Jiangtian
Shao, Yonghong
Li, Defa
Feng, Shuai
Liu, William J
He, Yaqing
Ma, Xiaopeng
Xie, Zhongjian
Zhang, Han
author_facet Chen, Zhi
Li, Jingfeng
Li, Tianzhong
Fan, Taojian
Meng, Changle
Li, Chaozhou
Kang, Jianlong
Chai, Luxiao
Hao, Yabin
Tang, Yuxuan
Al-Hartomy, Omar A
Wageh, Swelm
Al-Sehemi, Abdullah G
Luo, Zhiguang
Yu, Jiangtian
Shao, Yonghong
Li, Defa
Feng, Shuai
Liu, William J
He, Yaqing
Ma, Xiaopeng
Xie, Zhongjian
Zhang, Han
author_sort Chen, Zhi
collection PubMed
description The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed. On the other hand, several concerning SARS-CoV-2 variants, including Omicron, are required to be identified as soon as the samples are identified as ‘positive’. Unfortunately, a traditional PCR test does not allow their specific identification. Herein, for the first time, we have developed MOPCS (Methodologies of Photonic CRISPR Sensing), which combines an optical sensing technology-surface plasmon resonance (SPR) with the ‘gene scissors’ clustered regularly interspaced short palindromic repeat (CRISPR) technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants. MOPCS is a low-cost, CRISPR/Cas12a-system-empowered SPR gene-detecting platform that can analyze viral RNA, without the need for amplification, within 38 min from sample input to results output, and achieve a limit of detection of 15 fM. MOPCS achieves a highly sensitive analysis of SARS-CoV-2, and mutations appear in variants B.1.617.2 (Delta), B.1.1.529 (Omicron) and BA.1 (a subtype of Omicron). This platform was also used to analyze some recently collected patient samples from a local outbreak in China, identified by the Centers for Disease Control and Prevention. This innovative CRISPR-empowered SPR platform will further contribute to the fast, sensitive and accurate detection of target nucleic acid sequences with single-base mutations.
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spelling pubmed-93854562022-08-18 A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2 Chen, Zhi Li, Jingfeng Li, Tianzhong Fan, Taojian Meng, Changle Li, Chaozhou Kang, Jianlong Chai, Luxiao Hao, Yabin Tang, Yuxuan Al-Hartomy, Omar A Wageh, Swelm Al-Sehemi, Abdullah G Luo, Zhiguang Yu, Jiangtian Shao, Yonghong Li, Defa Feng, Shuai Liu, William J He, Yaqing Ma, Xiaopeng Xie, Zhongjian Zhang, Han Natl Sci Rev Research Article The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed. On the other hand, several concerning SARS-CoV-2 variants, including Omicron, are required to be identified as soon as the samples are identified as ‘positive’. Unfortunately, a traditional PCR test does not allow their specific identification. Herein, for the first time, we have developed MOPCS (Methodologies of Photonic CRISPR Sensing), which combines an optical sensing technology-surface plasmon resonance (SPR) with the ‘gene scissors’ clustered regularly interspaced short palindromic repeat (CRISPR) technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants. MOPCS is a low-cost, CRISPR/Cas12a-system-empowered SPR gene-detecting platform that can analyze viral RNA, without the need for amplification, within 38 min from sample input to results output, and achieve a limit of detection of 15 fM. MOPCS achieves a highly sensitive analysis of SARS-CoV-2, and mutations appear in variants B.1.617.2 (Delta), B.1.1.529 (Omicron) and BA.1 (a subtype of Omicron). This platform was also used to analyze some recently collected patient samples from a local outbreak in China, identified by the Centers for Disease Control and Prevention. This innovative CRISPR-empowered SPR platform will further contribute to the fast, sensitive and accurate detection of target nucleic acid sequences with single-base mutations. Oxford University Press 2022-06-03 /pmc/articles/PMC9385456/ /pubmed/35992231 http://dx.doi.org/10.1093/nsr/nwac104 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chen, Zhi
Li, Jingfeng
Li, Tianzhong
Fan, Taojian
Meng, Changle
Li, Chaozhou
Kang, Jianlong
Chai, Luxiao
Hao, Yabin
Tang, Yuxuan
Al-Hartomy, Omar A
Wageh, Swelm
Al-Sehemi, Abdullah G
Luo, Zhiguang
Yu, Jiangtian
Shao, Yonghong
Li, Defa
Feng, Shuai
Liu, William J
He, Yaqing
Ma, Xiaopeng
Xie, Zhongjian
Zhang, Han
A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2
title A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2
title_full A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2
title_fullStr A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2
title_full_unstemmed A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2
title_short A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2
title_sort crispr/cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the omicron variant of sars-cov-2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385456/
https://www.ncbi.nlm.nih.gov/pubmed/35992231
http://dx.doi.org/10.1093/nsr/nwac104
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