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Identification of a metabolic reprogramming‐related signature associated with prognosis and immune microenvironment of head and neck squamous cell carcinoma by in silico analysis

BACKGROUND: Metabolic reprogramming is one of the essential features of tumorigenesis. Herein, this study aimed to develop a novel metabolism‐related gene signature for head and neck squamous cell carcinoma (HNSCC) patients. METHODS: The transcriptomic and clinical data of HNSCC samples were collect...

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Autores principales: Qiang, Weijie, Dai, Yifei, Xing, Xiaoyan, Sun, Xiaobo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385599/
https://www.ncbi.nlm.nih.gov/pubmed/35301800
http://dx.doi.org/10.1002/cam4.4670
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author Qiang, Weijie
Dai, Yifei
Xing, Xiaoyan
Sun, Xiaobo
author_facet Qiang, Weijie
Dai, Yifei
Xing, Xiaoyan
Sun, Xiaobo
author_sort Qiang, Weijie
collection PubMed
description BACKGROUND: Metabolic reprogramming is one of the essential features of tumorigenesis. Herein, this study aimed to develop a novel metabolism‐related gene signature for head and neck squamous cell carcinoma (HNSCC) patients. METHODS: The transcriptomic and clinical data of HNSCC samples were collected from The Cancer Genome Atlas (TCGA) and GSE65858 datasets. The metabolism‐related gene‐based prognostic signature (MRGPS) was constructed by the Least Absolute Shrinkage and Selection Operator (LASSO) regression model. The time‐dependent receiver operating characteristic (ROC) and Kaplan‐Meier (K‐M) survival curves were plotted for evaluating its predicting performance. At the same time, univariate along with multivariate analysis was carried out to explore its correlation with clinicopathologic factors. Furthermore, GSEA analysis was performed to explore the signaling pathways affected by MRGPS. We also analyzed the associations of MRGPS with the tumor immune microenvironment (TIME), as well as identified potential compounds via Connectivity Map (CMap) and molecular docking. RESULTS: A total of 12 differentially expressed metabolism‐related genes were identified and selected to construct the MRGPS. Notably, this signature performed well in predicting HNSCC patients’ survival and could serve as an independent prognostic factor in multiple datasets. In addition to the metabolism‐related pathway, this signature could also affect some immune‐related pathways. The results indicated that MRGPS is correlated with immune cells infiltration and anti‐cancer immune response. Furthermore, we identified cephaeline as a potential therapeutic compound for HNSCC. CONCLUSION: Taken together, we established an MRGs‐based signature that has the potential to predict the clinical outcome and immune microenvironment, which help to search for potential combination immunotherapy compounds and provide a promising therapeutic strategy for treating HNSCC patients.
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spelling pubmed-93855992022-08-19 Identification of a metabolic reprogramming‐related signature associated with prognosis and immune microenvironment of head and neck squamous cell carcinoma by in silico analysis Qiang, Weijie Dai, Yifei Xing, Xiaoyan Sun, Xiaobo Cancer Med Research Articles BACKGROUND: Metabolic reprogramming is one of the essential features of tumorigenesis. Herein, this study aimed to develop a novel metabolism‐related gene signature for head and neck squamous cell carcinoma (HNSCC) patients. METHODS: The transcriptomic and clinical data of HNSCC samples were collected from The Cancer Genome Atlas (TCGA) and GSE65858 datasets. The metabolism‐related gene‐based prognostic signature (MRGPS) was constructed by the Least Absolute Shrinkage and Selection Operator (LASSO) regression model. The time‐dependent receiver operating characteristic (ROC) and Kaplan‐Meier (K‐M) survival curves were plotted for evaluating its predicting performance. At the same time, univariate along with multivariate analysis was carried out to explore its correlation with clinicopathologic factors. Furthermore, GSEA analysis was performed to explore the signaling pathways affected by MRGPS. We also analyzed the associations of MRGPS with the tumor immune microenvironment (TIME), as well as identified potential compounds via Connectivity Map (CMap) and molecular docking. RESULTS: A total of 12 differentially expressed metabolism‐related genes were identified and selected to construct the MRGPS. Notably, this signature performed well in predicting HNSCC patients’ survival and could serve as an independent prognostic factor in multiple datasets. In addition to the metabolism‐related pathway, this signature could also affect some immune‐related pathways. The results indicated that MRGPS is correlated with immune cells infiltration and anti‐cancer immune response. Furthermore, we identified cephaeline as a potential therapeutic compound for HNSCC. CONCLUSION: Taken together, we established an MRGs‐based signature that has the potential to predict the clinical outcome and immune microenvironment, which help to search for potential combination immunotherapy compounds and provide a promising therapeutic strategy for treating HNSCC patients. John Wiley and Sons Inc. 2022-03-18 /pmc/articles/PMC9385599/ /pubmed/35301800 http://dx.doi.org/10.1002/cam4.4670 Text en © 2022 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Qiang, Weijie
Dai, Yifei
Xing, Xiaoyan
Sun, Xiaobo
Identification of a metabolic reprogramming‐related signature associated with prognosis and immune microenvironment of head and neck squamous cell carcinoma by in silico analysis
title Identification of a metabolic reprogramming‐related signature associated with prognosis and immune microenvironment of head and neck squamous cell carcinoma by in silico analysis
title_full Identification of a metabolic reprogramming‐related signature associated with prognosis and immune microenvironment of head and neck squamous cell carcinoma by in silico analysis
title_fullStr Identification of a metabolic reprogramming‐related signature associated with prognosis and immune microenvironment of head and neck squamous cell carcinoma by in silico analysis
title_full_unstemmed Identification of a metabolic reprogramming‐related signature associated with prognosis and immune microenvironment of head and neck squamous cell carcinoma by in silico analysis
title_short Identification of a metabolic reprogramming‐related signature associated with prognosis and immune microenvironment of head and neck squamous cell carcinoma by in silico analysis
title_sort identification of a metabolic reprogramming‐related signature associated with prognosis and immune microenvironment of head and neck squamous cell carcinoma by in silico analysis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385599/
https://www.ncbi.nlm.nih.gov/pubmed/35301800
http://dx.doi.org/10.1002/cam4.4670
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