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Intrinsic cell rheology drives junction maturation
A fundamental property of higher eukaryotes that underpins their evolutionary success is stable cell-cell cohesion. Yet, how intrinsic cell rheology and stiffness contributes to junction stabilization and maturation is poorly understood. We demonstrate that localized modulation of cell rheology gove...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385638/ https://www.ncbi.nlm.nih.gov/pubmed/35977954 http://dx.doi.org/10.1038/s41467-022-32102-9 |
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author | Sri-Ranjan, K. Sanchez-Alonso, J. L. Swiatlowska, P. Rothery, S. Novak, P. Gerlach, S. Koeninger, D. Hoffmann, B. Merkel, R. Stevens, M. M. Sun, S. X. Gorelik, J. Braga, Vania M. M. |
author_facet | Sri-Ranjan, K. Sanchez-Alonso, J. L. Swiatlowska, P. Rothery, S. Novak, P. Gerlach, S. Koeninger, D. Hoffmann, B. Merkel, R. Stevens, M. M. Sun, S. X. Gorelik, J. Braga, Vania M. M. |
author_sort | Sri-Ranjan, K. |
collection | PubMed |
description | A fundamental property of higher eukaryotes that underpins their evolutionary success is stable cell-cell cohesion. Yet, how intrinsic cell rheology and stiffness contributes to junction stabilization and maturation is poorly understood. We demonstrate that localized modulation of cell rheology governs the transition of a slack, undulated cell-cell contact (weak adhesion) to a mature, straight junction (optimal adhesion). Cell pairs confined on different geometries have heterogeneous elasticity maps and control their own intrinsic rheology co-ordinately. More compliant cell pairs grown on circles have slack contacts, while stiffer triangular cell pairs favour straight junctions with flanking contractile thin bundles. Counter-intuitively, straighter cell-cell contacts have reduced receptor density and less dynamic junctional actin, suggesting an unusual adaptive mechano-response to stabilize cell-cell adhesion. Our modelling informs that slack junctions arise from failure of circular cell pairs to increase their own intrinsic stiffness and resist the pressures from the neighbouring cell. The inability to form a straight junction can be reversed by increasing mechanical stress artificially on stiffer substrates. Our data inform on the minimal intrinsic rheology to generate a mature junction and provide a springboard towards understanding elements governing tissue-level mechanics. |
format | Online Article Text |
id | pubmed-9385638 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-93856382022-08-19 Intrinsic cell rheology drives junction maturation Sri-Ranjan, K. Sanchez-Alonso, J. L. Swiatlowska, P. Rothery, S. Novak, P. Gerlach, S. Koeninger, D. Hoffmann, B. Merkel, R. Stevens, M. M. Sun, S. X. Gorelik, J. Braga, Vania M. M. Nat Commun Article A fundamental property of higher eukaryotes that underpins their evolutionary success is stable cell-cell cohesion. Yet, how intrinsic cell rheology and stiffness contributes to junction stabilization and maturation is poorly understood. We demonstrate that localized modulation of cell rheology governs the transition of a slack, undulated cell-cell contact (weak adhesion) to a mature, straight junction (optimal adhesion). Cell pairs confined on different geometries have heterogeneous elasticity maps and control their own intrinsic rheology co-ordinately. More compliant cell pairs grown on circles have slack contacts, while stiffer triangular cell pairs favour straight junctions with flanking contractile thin bundles. Counter-intuitively, straighter cell-cell contacts have reduced receptor density and less dynamic junctional actin, suggesting an unusual adaptive mechano-response to stabilize cell-cell adhesion. Our modelling informs that slack junctions arise from failure of circular cell pairs to increase their own intrinsic stiffness and resist the pressures from the neighbouring cell. The inability to form a straight junction can be reversed by increasing mechanical stress artificially on stiffer substrates. Our data inform on the minimal intrinsic rheology to generate a mature junction and provide a springboard towards understanding elements governing tissue-level mechanics. Nature Publishing Group UK 2022-08-17 /pmc/articles/PMC9385638/ /pubmed/35977954 http://dx.doi.org/10.1038/s41467-022-32102-9 Text en © Crown 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Sri-Ranjan, K. Sanchez-Alonso, J. L. Swiatlowska, P. Rothery, S. Novak, P. Gerlach, S. Koeninger, D. Hoffmann, B. Merkel, R. Stevens, M. M. Sun, S. X. Gorelik, J. Braga, Vania M. M. Intrinsic cell rheology drives junction maturation |
title | Intrinsic cell rheology drives junction maturation |
title_full | Intrinsic cell rheology drives junction maturation |
title_fullStr | Intrinsic cell rheology drives junction maturation |
title_full_unstemmed | Intrinsic cell rheology drives junction maturation |
title_short | Intrinsic cell rheology drives junction maturation |
title_sort | intrinsic cell rheology drives junction maturation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385638/ https://www.ncbi.nlm.nih.gov/pubmed/35977954 http://dx.doi.org/10.1038/s41467-022-32102-9 |
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