Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells

The human pluripotent stem cell (hPSC) differentiation has allowed for the generation of in vitro models to study human diseases in a dish. This protocol describes the generation of keratinocyte-like cells from hPSCs in chemically defined media. Treating hPSCs with retinoic acid and BMP4 induced the...

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Detalles Bibliográficos
Autores principales: Ali, Gowher, Abdelalim, Essam M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385691/
https://www.ncbi.nlm.nih.gov/pubmed/35990735
http://dx.doi.org/10.1016/j.xpro.2022.101613
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author Ali, Gowher
Abdelalim, Essam M.
author_facet Ali, Gowher
Abdelalim, Essam M.
author_sort Ali, Gowher
collection PubMed
description The human pluripotent stem cell (hPSC) differentiation has allowed for the generation of in vitro models to study human diseases in a dish. This protocol describes the generation of keratinocyte-like cells from hPSCs in chemically defined media. Treating hPSCs with retinoic acid and BMP4 induced the generation of keratinocyte progenitors, which further differentiated into mature keratinocytes in the presence of calcium. The keratinocytes generated with this protocol could be used to study keratinocyte biology, drug screening, and skin-related diseases. For complete details on the use and execution of this protocol, please refer to Ali et al. (2020).
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spelling pubmed-93856912022-08-19 Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells Ali, Gowher Abdelalim, Essam M. STAR Protoc Protocol The human pluripotent stem cell (hPSC) differentiation has allowed for the generation of in vitro models to study human diseases in a dish. This protocol describes the generation of keratinocyte-like cells from hPSCs in chemically defined media. Treating hPSCs with retinoic acid and BMP4 induced the generation of keratinocyte progenitors, which further differentiated into mature keratinocytes in the presence of calcium. The keratinocytes generated with this protocol could be used to study keratinocyte biology, drug screening, and skin-related diseases. For complete details on the use and execution of this protocol, please refer to Ali et al. (2020). Elsevier 2022-08-08 /pmc/articles/PMC9385691/ /pubmed/35990735 http://dx.doi.org/10.1016/j.xpro.2022.101613 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Ali, Gowher
Abdelalim, Essam M.
Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells
title Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells
title_full Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells
title_fullStr Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells
title_full_unstemmed Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells
title_short Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells
title_sort directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385691/
https://www.ncbi.nlm.nih.gov/pubmed/35990735
http://dx.doi.org/10.1016/j.xpro.2022.101613
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