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Biological aspects of phage therapy versus antibiotics against Salmonella enterica serovar Typhimurium infection of chickens

Phage therapy is a promising alternative treatment of bacterial infections in human and animals. Nevertheless, despite the appearance of many bacterial strains resistant to antibiotics, these drugs still remain important therapeutics used in human and veterinary medicine. Although experimental phage...

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Autores principales: Kosznik-Kwaśnicka, Katarzyna, Podlacha, Magdalena, Grabowski, Łukasz, Stasiłojć, Małgorzata, Nowak-Zaleska, Alicja, Ciemińska, Karolina, Cyske, Zuzanna, Dydecka, Aleksandra, Gaffke, Lidia, Mantej, Jagoda, Myślińska, Dorota, Necel, Agnieszka, Pierzynowska, Karolina, Piotrowska, Ewa, Radzanowska-Alenowicz, Edyta, Rintz, Estera, Sitko, Krzysztof, Topka-Bielecka, Gracja, Węgrzyn, Grzegorz, Węgrzyn, Alicja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385949/
https://www.ncbi.nlm.nih.gov/pubmed/35992162
http://dx.doi.org/10.3389/fcimb.2022.941867
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author Kosznik-Kwaśnicka, Katarzyna
Podlacha, Magdalena
Grabowski, Łukasz
Stasiłojć, Małgorzata
Nowak-Zaleska, Alicja
Ciemińska, Karolina
Cyske, Zuzanna
Dydecka, Aleksandra
Gaffke, Lidia
Mantej, Jagoda
Myślińska, Dorota
Necel, Agnieszka
Pierzynowska, Karolina
Piotrowska, Ewa
Radzanowska-Alenowicz, Edyta
Rintz, Estera
Sitko, Krzysztof
Topka-Bielecka, Gracja
Węgrzyn, Grzegorz
Węgrzyn, Alicja
author_facet Kosznik-Kwaśnicka, Katarzyna
Podlacha, Magdalena
Grabowski, Łukasz
Stasiłojć, Małgorzata
Nowak-Zaleska, Alicja
Ciemińska, Karolina
Cyske, Zuzanna
Dydecka, Aleksandra
Gaffke, Lidia
Mantej, Jagoda
Myślińska, Dorota
Necel, Agnieszka
Pierzynowska, Karolina
Piotrowska, Ewa
Radzanowska-Alenowicz, Edyta
Rintz, Estera
Sitko, Krzysztof
Topka-Bielecka, Gracja
Węgrzyn, Grzegorz
Węgrzyn, Alicja
author_sort Kosznik-Kwaśnicka, Katarzyna
collection PubMed
description Phage therapy is a promising alternative treatment of bacterial infections in human and animals. Nevertheless, despite the appearance of many bacterial strains resistant to antibiotics, these drugs still remain important therapeutics used in human and veterinary medicine. Although experimental phage therapy of infections caused by Salmonella enterica was described previously by many groups, those studies focused solely on effects caused by bacteriophages. Here, we compared the use of phage therapy (employing a cocktail composed of two previously isolated and characterized bacteriophages, vB_SenM-2 and vB_Sen-TO17) and antibiotics (enrofloxacin and colistin) in chickens infected experimentally with S. enterica serovar Typhimurium. We found that the efficacies of both types of therapies (i.e. the use of antibiotics and phage cocktail) were high and very similar to one another when the treatment was applied shortly (one day) after the infection. Under these conditions, S. Typhimurium was quickly eliminated from the gastrointestinal tract (GIT), to the amount not detectable by the used methods. However, later treatment (2 or 4 days after detection of S. Typhimurium in chicken feces) with the phage cocktail was significantly less effective. Bacteriophages remained in the GIT for up to 2-3 weeks, and then were absent in feces and cloaca swabs. Interestingly, both phages could be found in various organs of chickens though with a relatively low abundance. No development of resistance of S. Typhimurium to phages or antibiotics was detected during the experiment. Importantly, although antibiotics significantly changed the GIT microbiome of chickens in a long-term manner, analogous changes caused by phages were transient, and the microbiome normalized a few weeks after the treatment. In conclusion, phage therapy against S. Typhimurium infection in chickens appeared as effective as antibiotic therapy (with either enrofloxacin or colistin), and less invasive than the use the antibiotics as fewer changes in the microbiome were observed.
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spelling pubmed-93859492022-08-19 Biological aspects of phage therapy versus antibiotics against Salmonella enterica serovar Typhimurium infection of chickens Kosznik-Kwaśnicka, Katarzyna Podlacha, Magdalena Grabowski, Łukasz Stasiłojć, Małgorzata Nowak-Zaleska, Alicja Ciemińska, Karolina Cyske, Zuzanna Dydecka, Aleksandra Gaffke, Lidia Mantej, Jagoda Myślińska, Dorota Necel, Agnieszka Pierzynowska, Karolina Piotrowska, Ewa Radzanowska-Alenowicz, Edyta Rintz, Estera Sitko, Krzysztof Topka-Bielecka, Gracja Węgrzyn, Grzegorz Węgrzyn, Alicja Front Cell Infect Microbiol Cellular and Infection Microbiology Phage therapy is a promising alternative treatment of bacterial infections in human and animals. Nevertheless, despite the appearance of many bacterial strains resistant to antibiotics, these drugs still remain important therapeutics used in human and veterinary medicine. Although experimental phage therapy of infections caused by Salmonella enterica was described previously by many groups, those studies focused solely on effects caused by bacteriophages. Here, we compared the use of phage therapy (employing a cocktail composed of two previously isolated and characterized bacteriophages, vB_SenM-2 and vB_Sen-TO17) and antibiotics (enrofloxacin and colistin) in chickens infected experimentally with S. enterica serovar Typhimurium. We found that the efficacies of both types of therapies (i.e. the use of antibiotics and phage cocktail) were high and very similar to one another when the treatment was applied shortly (one day) after the infection. Under these conditions, S. Typhimurium was quickly eliminated from the gastrointestinal tract (GIT), to the amount not detectable by the used methods. However, later treatment (2 or 4 days after detection of S. Typhimurium in chicken feces) with the phage cocktail was significantly less effective. Bacteriophages remained in the GIT for up to 2-3 weeks, and then were absent in feces and cloaca swabs. Interestingly, both phages could be found in various organs of chickens though with a relatively low abundance. No development of resistance of S. Typhimurium to phages or antibiotics was detected during the experiment. Importantly, although antibiotics significantly changed the GIT microbiome of chickens in a long-term manner, analogous changes caused by phages were transient, and the microbiome normalized a few weeks after the treatment. In conclusion, phage therapy against S. Typhimurium infection in chickens appeared as effective as antibiotic therapy (with either enrofloxacin or colistin), and less invasive than the use the antibiotics as fewer changes in the microbiome were observed. Frontiers Media S.A. 2022-08-04 /pmc/articles/PMC9385949/ /pubmed/35992162 http://dx.doi.org/10.3389/fcimb.2022.941867 Text en Copyright © 2022 Kosznik-Kwaśnicka, Podlacha, Grabowski, Stasiłojć, Nowak-Zaleska, Ciemińska, Cyske, Dydecka, Gaffke, Mantej, Myślińska, Necel, Pierzynowska, Piotrowska, Radzanowska-Alenowicz, Rintz, Sitko, Topka-Bielecka, Węgrzyn and Węgrzyn https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Kosznik-Kwaśnicka, Katarzyna
Podlacha, Magdalena
Grabowski, Łukasz
Stasiłojć, Małgorzata
Nowak-Zaleska, Alicja
Ciemińska, Karolina
Cyske, Zuzanna
Dydecka, Aleksandra
Gaffke, Lidia
Mantej, Jagoda
Myślińska, Dorota
Necel, Agnieszka
Pierzynowska, Karolina
Piotrowska, Ewa
Radzanowska-Alenowicz, Edyta
Rintz, Estera
Sitko, Krzysztof
Topka-Bielecka, Gracja
Węgrzyn, Grzegorz
Węgrzyn, Alicja
Biological aspects of phage therapy versus antibiotics against Salmonella enterica serovar Typhimurium infection of chickens
title Biological aspects of phage therapy versus antibiotics against Salmonella enterica serovar Typhimurium infection of chickens
title_full Biological aspects of phage therapy versus antibiotics against Salmonella enterica serovar Typhimurium infection of chickens
title_fullStr Biological aspects of phage therapy versus antibiotics against Salmonella enterica serovar Typhimurium infection of chickens
title_full_unstemmed Biological aspects of phage therapy versus antibiotics against Salmonella enterica serovar Typhimurium infection of chickens
title_short Biological aspects of phage therapy versus antibiotics against Salmonella enterica serovar Typhimurium infection of chickens
title_sort biological aspects of phage therapy versus antibiotics against salmonella enterica serovar typhimurium infection of chickens
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385949/
https://www.ncbi.nlm.nih.gov/pubmed/35992162
http://dx.doi.org/10.3389/fcimb.2022.941867
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