Cargando…
Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing
Durable crop disease resistance is an essential component of global food security. Continuous pathogen evolution leads to a breakdown of resistance and there is a pressing need to characterize new resistance genes for use in plant breeding. Here we identified an accession of wild emmer wheat (Tritic...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386016/ https://www.ncbi.nlm.nih.gov/pubmed/35978056 http://dx.doi.org/10.1038/s42003-022-03773-3 |
_version_ | 1784769707625152512 |
---|---|
author | Klymiuk, Valentyna Chawla, Harmeet Singh Wiebe, Krystalee Ens, Jennifer Fatiukha, Andrii Govta, Liubov Fahima, Tzion Pozniak, Curtis J. |
author_facet | Klymiuk, Valentyna Chawla, Harmeet Singh Wiebe, Krystalee Ens, Jennifer Fatiukha, Andrii Govta, Liubov Fahima, Tzion Pozniak, Curtis J. |
author_sort | Klymiuk, Valentyna |
collection | PubMed |
description | Durable crop disease resistance is an essential component of global food security. Continuous pathogen evolution leads to a breakdown of resistance and there is a pressing need to characterize new resistance genes for use in plant breeding. Here we identified an accession of wild emmer wheat (Triticum turgidum ssp. dicoccoides), PI 487260, that is highly resistant to multiple stripe rust isolates. Genetic analysis revealed resistance was conferred by a single, incompletely dominant gene designated as Yr84. Through bulked segregant analysis sequencing (BSA-Seq) we identified a 52.7 Mb resistance-associated interval on chromosome 1BS. Detected variants were used to design genetic markers for recombinant screening, further refining the interval of Yr84 to a 2.3–3.3 Mb in tetraploid wheat genomes. This interval contains 34 candidate genes encoding for protein domains involved in disease resistance responses. Furthermore, KASP markers closely-linked to Yr84 were developed to facilitate marker-assisted selection for rust resistance breeding. |
format | Online Article Text |
id | pubmed-9386016 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-93860162022-08-19 Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing Klymiuk, Valentyna Chawla, Harmeet Singh Wiebe, Krystalee Ens, Jennifer Fatiukha, Andrii Govta, Liubov Fahima, Tzion Pozniak, Curtis J. Commun Biol Article Durable crop disease resistance is an essential component of global food security. Continuous pathogen evolution leads to a breakdown of resistance and there is a pressing need to characterize new resistance genes for use in plant breeding. Here we identified an accession of wild emmer wheat (Triticum turgidum ssp. dicoccoides), PI 487260, that is highly resistant to multiple stripe rust isolates. Genetic analysis revealed resistance was conferred by a single, incompletely dominant gene designated as Yr84. Through bulked segregant analysis sequencing (BSA-Seq) we identified a 52.7 Mb resistance-associated interval on chromosome 1BS. Detected variants were used to design genetic markers for recombinant screening, further refining the interval of Yr84 to a 2.3–3.3 Mb in tetraploid wheat genomes. This interval contains 34 candidate genes encoding for protein domains involved in disease resistance responses. Furthermore, KASP markers closely-linked to Yr84 were developed to facilitate marker-assisted selection for rust resistance breeding. Nature Publishing Group UK 2022-08-17 /pmc/articles/PMC9386016/ /pubmed/35978056 http://dx.doi.org/10.1038/s42003-022-03773-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Klymiuk, Valentyna Chawla, Harmeet Singh Wiebe, Krystalee Ens, Jennifer Fatiukha, Andrii Govta, Liubov Fahima, Tzion Pozniak, Curtis J. Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing |
title | Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing |
title_full | Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing |
title_fullStr | Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing |
title_full_unstemmed | Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing |
title_short | Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing |
title_sort | discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386016/ https://www.ncbi.nlm.nih.gov/pubmed/35978056 http://dx.doi.org/10.1038/s42003-022-03773-3 |
work_keys_str_mv | AT klymiukvalentyna discoveryofstriperustresistancewithincompletedominanceinwildemmerwheatusingbulkedsegregantanalysissequencing AT chawlaharmeetsingh discoveryofstriperustresistancewithincompletedominanceinwildemmerwheatusingbulkedsegregantanalysissequencing AT wiebekrystalee discoveryofstriperustresistancewithincompletedominanceinwildemmerwheatusingbulkedsegregantanalysissequencing AT ensjennifer discoveryofstriperustresistancewithincompletedominanceinwildemmerwheatusingbulkedsegregantanalysissequencing AT fatiukhaandrii discoveryofstriperustresistancewithincompletedominanceinwildemmerwheatusingbulkedsegregantanalysissequencing AT govtaliubov discoveryofstriperustresistancewithincompletedominanceinwildemmerwheatusingbulkedsegregantanalysissequencing AT fahimatzion discoveryofstriperustresistancewithincompletedominanceinwildemmerwheatusingbulkedsegregantanalysissequencing AT pozniakcurtisj discoveryofstriperustresistancewithincompletedominanceinwildemmerwheatusingbulkedsegregantanalysissequencing |