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Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing

Durable crop disease resistance is an essential component of global food security. Continuous pathogen evolution leads to a breakdown of resistance and there is a pressing need to characterize new resistance genes for use in plant breeding. Here we identified an accession of wild emmer wheat (Tritic...

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Autores principales: Klymiuk, Valentyna, Chawla, Harmeet Singh, Wiebe, Krystalee, Ens, Jennifer, Fatiukha, Andrii, Govta, Liubov, Fahima, Tzion, Pozniak, Curtis J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386016/
https://www.ncbi.nlm.nih.gov/pubmed/35978056
http://dx.doi.org/10.1038/s42003-022-03773-3
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author Klymiuk, Valentyna
Chawla, Harmeet Singh
Wiebe, Krystalee
Ens, Jennifer
Fatiukha, Andrii
Govta, Liubov
Fahima, Tzion
Pozniak, Curtis J.
author_facet Klymiuk, Valentyna
Chawla, Harmeet Singh
Wiebe, Krystalee
Ens, Jennifer
Fatiukha, Andrii
Govta, Liubov
Fahima, Tzion
Pozniak, Curtis J.
author_sort Klymiuk, Valentyna
collection PubMed
description Durable crop disease resistance is an essential component of global food security. Continuous pathogen evolution leads to a breakdown of resistance and there is a pressing need to characterize new resistance genes for use in plant breeding. Here we identified an accession of wild emmer wheat (Triticum turgidum ssp. dicoccoides), PI 487260, that is highly resistant to multiple stripe rust isolates. Genetic analysis revealed resistance was conferred by a single, incompletely dominant gene designated as Yr84. Through bulked segregant analysis sequencing (BSA-Seq) we identified a 52.7 Mb resistance-associated interval on chromosome 1BS. Detected variants were used to design genetic markers for recombinant screening, further refining the interval of Yr84 to a 2.3–3.3 Mb in tetraploid wheat genomes. This interval contains 34 candidate genes encoding for protein domains involved in disease resistance responses. Furthermore, KASP markers closely-linked to Yr84 were developed to facilitate marker-assisted selection for rust resistance breeding.
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spelling pubmed-93860162022-08-19 Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing Klymiuk, Valentyna Chawla, Harmeet Singh Wiebe, Krystalee Ens, Jennifer Fatiukha, Andrii Govta, Liubov Fahima, Tzion Pozniak, Curtis J. Commun Biol Article Durable crop disease resistance is an essential component of global food security. Continuous pathogen evolution leads to a breakdown of resistance and there is a pressing need to characterize new resistance genes for use in plant breeding. Here we identified an accession of wild emmer wheat (Triticum turgidum ssp. dicoccoides), PI 487260, that is highly resistant to multiple stripe rust isolates. Genetic analysis revealed resistance was conferred by a single, incompletely dominant gene designated as Yr84. Through bulked segregant analysis sequencing (BSA-Seq) we identified a 52.7 Mb resistance-associated interval on chromosome 1BS. Detected variants were used to design genetic markers for recombinant screening, further refining the interval of Yr84 to a 2.3–3.3 Mb in tetraploid wheat genomes. This interval contains 34 candidate genes encoding for protein domains involved in disease resistance responses. Furthermore, KASP markers closely-linked to Yr84 were developed to facilitate marker-assisted selection for rust resistance breeding. Nature Publishing Group UK 2022-08-17 /pmc/articles/PMC9386016/ /pubmed/35978056 http://dx.doi.org/10.1038/s42003-022-03773-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Klymiuk, Valentyna
Chawla, Harmeet Singh
Wiebe, Krystalee
Ens, Jennifer
Fatiukha, Andrii
Govta, Liubov
Fahima, Tzion
Pozniak, Curtis J.
Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing
title Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing
title_full Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing
title_fullStr Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing
title_full_unstemmed Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing
title_short Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing
title_sort discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386016/
https://www.ncbi.nlm.nih.gov/pubmed/35978056
http://dx.doi.org/10.1038/s42003-022-03773-3
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