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Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients
Osteosarcoma (OS) is a rare malignant primary bone tumours characterized by a high genetic and cell composition heterogeneity. Unfortunately, despite the use of drug combinations and the recent development of immunotherapies, the overall survival has not improved in the last four decades. Due to the...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386085/ https://www.ncbi.nlm.nih.gov/pubmed/35990515 http://dx.doi.org/10.1016/j.jbo.2022.100451 |
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author | Heymann, Clément J.F. Bobin-Dubigeon, Christine Muñoz-Garcia, Javier Cochonneau, Denis Ollivier, Emilie Heymann, Marie-Françoise Heymann, Dominique |
author_facet | Heymann, Clément J.F. Bobin-Dubigeon, Christine Muñoz-Garcia, Javier Cochonneau, Denis Ollivier, Emilie Heymann, Marie-Françoise Heymann, Dominique |
author_sort | Heymann, Clément J.F. |
collection | PubMed |
description | Osteosarcoma (OS) is a rare malignant primary bone tumours characterized by a high genetic and cell composition heterogeneity. Unfortunately, despite the use of drug combinations and the recent development of immunotherapies, the overall survival has not improved in the last four decades. Due to the key role of the tumour microenvironment in the pathogenesis of OS, a better understanding of its microenvironment is mandatory to develop new therapeutic approaches. From retrospective biological cohorts of OS, we analysed by immunohistochemistry the presence of lipopolysaccharide (LPS)-binding protein (LBP) in diagnostic biopsies with local disease and compared their level of infiltration to patients suffering from metastatic status. LBP is considered as a marker of LPS exposure and can indirectly reflect the presence of Gram-negative microbiota. LBP were detected in the cytoplasm of OS cells as well as in tumour-associated macrophage. Tumour samples of patients with local disease were significantly enriched in LBP compared to tumour tissues of patients with metastatic status. Lung metastatic tissues showed similar level of LBP compared to paired primary tumours. Overall, this study strongly suggests the presence of Gram-negative bacteria in OS tissues and demonstrated their significant differential level according the metastatic status. This tumour-associated microbiome may help in the conceptualisation of new therapeutic approach to trigger efficient therapeutic responses against cancer. |
format | Online Article Text |
id | pubmed-9386085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-93860852022-08-19 Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients Heymann, Clément J.F. Bobin-Dubigeon, Christine Muñoz-Garcia, Javier Cochonneau, Denis Ollivier, Emilie Heymann, Marie-Françoise Heymann, Dominique J Bone Oncol Research Paper Osteosarcoma (OS) is a rare malignant primary bone tumours characterized by a high genetic and cell composition heterogeneity. Unfortunately, despite the use of drug combinations and the recent development of immunotherapies, the overall survival has not improved in the last four decades. Due to the key role of the tumour microenvironment in the pathogenesis of OS, a better understanding of its microenvironment is mandatory to develop new therapeutic approaches. From retrospective biological cohorts of OS, we analysed by immunohistochemistry the presence of lipopolysaccharide (LPS)-binding protein (LBP) in diagnostic biopsies with local disease and compared their level of infiltration to patients suffering from metastatic status. LBP is considered as a marker of LPS exposure and can indirectly reflect the presence of Gram-negative microbiota. LBP were detected in the cytoplasm of OS cells as well as in tumour-associated macrophage. Tumour samples of patients with local disease were significantly enriched in LBP compared to tumour tissues of patients with metastatic status. Lung metastatic tissues showed similar level of LBP compared to paired primary tumours. Overall, this study strongly suggests the presence of Gram-negative bacteria in OS tissues and demonstrated their significant differential level according the metastatic status. This tumour-associated microbiome may help in the conceptualisation of new therapeutic approach to trigger efficient therapeutic responses against cancer. Elsevier 2022-08-04 /pmc/articles/PMC9386085/ /pubmed/35990515 http://dx.doi.org/10.1016/j.jbo.2022.100451 Text en © 2022 The Authors. Published by Elsevier GmbH. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Paper Heymann, Clément J.F. Bobin-Dubigeon, Christine Muñoz-Garcia, Javier Cochonneau, Denis Ollivier, Emilie Heymann, Marie-Françoise Heymann, Dominique Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients |
title | Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients |
title_full | Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients |
title_fullStr | Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients |
title_full_unstemmed | Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients |
title_short | Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients |
title_sort | lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386085/ https://www.ncbi.nlm.nih.gov/pubmed/35990515 http://dx.doi.org/10.1016/j.jbo.2022.100451 |
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