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Comparison and optimization of DNA Isolation protocols for high throughput genomic studies of Acacia pachyceras Schwartz

We describe the optimization and validation of six DNA isolation protocols from fresh leaves of the rare tree Acacia pachyceras. The first four protocols employed three commercial kits (Sigma, Nucleospin1, Nucleospin 2, Promega) whereas the remaining two were based on the traditional sodium dodecyl...

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Detalles Bibliográficos
Autores principales: Habibi, Nazima, Al Salameen, Fadila, Rahman, Muhammed, Shajan, Anisha, Zakir, Farhana, Abdulrazzack, Nasreem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386096/
https://www.ncbi.nlm.nih.gov/pubmed/35990814
http://dx.doi.org/10.1016/j.mex.2022.101799