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Transcriptome and metabolome analyses reveal transcription factors regulating ganoderic acid biosynthesis in Ganoderma lucidum development

Ganoderma lucidum is an important medicinal fungus in Asian countries. Ganoderic acid (GA) is the major variety of bioactive and medicative components in G. lucidum. Biosynthesis of secondary metabolites is usually associated with cell differentiation and development. However, the mechanism underlyi...

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Autores principales: Meng, Li, Zhou, Ruyue, Lin, Jialong, Zang, Xizhe, Wang, Qingji, Wang, Panmeng, Wang, Li, Li, Zhuang, Wang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386254/
https://www.ncbi.nlm.nih.gov/pubmed/35992655
http://dx.doi.org/10.3389/fmicb.2022.956421
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author Meng, Li
Zhou, Ruyue
Lin, Jialong
Zang, Xizhe
Wang, Qingji
Wang, Panmeng
Wang, Li
Li, Zhuang
Wang, Wei
author_facet Meng, Li
Zhou, Ruyue
Lin, Jialong
Zang, Xizhe
Wang, Qingji
Wang, Panmeng
Wang, Li
Li, Zhuang
Wang, Wei
author_sort Meng, Li
collection PubMed
description Ganoderma lucidum is an important medicinal fungus in Asian countries. Ganoderic acid (GA) is the major variety of bioactive and medicative components in G. lucidum. Biosynthesis of secondary metabolites is usually associated with cell differentiation and development. However, the mechanism underlying these phenomena remain unclear. Transcription factors play an essential regulatory role in the signal transduction pathway, owing to the fact that they represent the major link between signal transduction and expression of target genes. In the present study, we performed transcriptome and metabolome analyses to identify transcription factors involved in GA biosynthesis during development of G. lucidum. Transcriptome data revealed differentially expressed genes between mycelia and primordia, as well as between mycelia and the fruiting body. Results from gene ontology enrichment analysis and metabolome analyses suggested that GAs and flavonoids biosynthetic process significantly changed during fungal development. The analysis of predicted occurrences of DNA-binding domains revealed a set of 53 potential transcription factor families in G. lucidum. Notably, we found homeobox transcription factor and velvet family protein played important role in GA biosynthesis. Combined with previous studies, we provided a model diagram of transcription factors involved in GA biosynthesis during fruiting body formation. Collectively, these results are expected to enhance our understanding into the mechanisms underlying secondary metabolite biosynthesis and development in fungi.
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spelling pubmed-93862542022-08-19 Transcriptome and metabolome analyses reveal transcription factors regulating ganoderic acid biosynthesis in Ganoderma lucidum development Meng, Li Zhou, Ruyue Lin, Jialong Zang, Xizhe Wang, Qingji Wang, Panmeng Wang, Li Li, Zhuang Wang, Wei Front Microbiol Microbiology Ganoderma lucidum is an important medicinal fungus in Asian countries. Ganoderic acid (GA) is the major variety of bioactive and medicative components in G. lucidum. Biosynthesis of secondary metabolites is usually associated with cell differentiation and development. However, the mechanism underlying these phenomena remain unclear. Transcription factors play an essential regulatory role in the signal transduction pathway, owing to the fact that they represent the major link between signal transduction and expression of target genes. In the present study, we performed transcriptome and metabolome analyses to identify transcription factors involved in GA biosynthesis during development of G. lucidum. Transcriptome data revealed differentially expressed genes between mycelia and primordia, as well as between mycelia and the fruiting body. Results from gene ontology enrichment analysis and metabolome analyses suggested that GAs and flavonoids biosynthetic process significantly changed during fungal development. The analysis of predicted occurrences of DNA-binding domains revealed a set of 53 potential transcription factor families in G. lucidum. Notably, we found homeobox transcription factor and velvet family protein played important role in GA biosynthesis. Combined with previous studies, we provided a model diagram of transcription factors involved in GA biosynthesis during fruiting body formation. Collectively, these results are expected to enhance our understanding into the mechanisms underlying secondary metabolite biosynthesis and development in fungi. Frontiers Media S.A. 2022-08-04 /pmc/articles/PMC9386254/ /pubmed/35992655 http://dx.doi.org/10.3389/fmicb.2022.956421 Text en Copyright © 2022 Meng, Zhou, Lin, Zang, Wang, Wang, Wang, Li and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Meng, Li
Zhou, Ruyue
Lin, Jialong
Zang, Xizhe
Wang, Qingji
Wang, Panmeng
Wang, Li
Li, Zhuang
Wang, Wei
Transcriptome and metabolome analyses reveal transcription factors regulating ganoderic acid biosynthesis in Ganoderma lucidum development
title Transcriptome and metabolome analyses reveal transcription factors regulating ganoderic acid biosynthesis in Ganoderma lucidum development
title_full Transcriptome and metabolome analyses reveal transcription factors regulating ganoderic acid biosynthesis in Ganoderma lucidum development
title_fullStr Transcriptome and metabolome analyses reveal transcription factors regulating ganoderic acid biosynthesis in Ganoderma lucidum development
title_full_unstemmed Transcriptome and metabolome analyses reveal transcription factors regulating ganoderic acid biosynthesis in Ganoderma lucidum development
title_short Transcriptome and metabolome analyses reveal transcription factors regulating ganoderic acid biosynthesis in Ganoderma lucidum development
title_sort transcriptome and metabolome analyses reveal transcription factors regulating ganoderic acid biosynthesis in ganoderma lucidum development
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386254/
https://www.ncbi.nlm.nih.gov/pubmed/35992655
http://dx.doi.org/10.3389/fmicb.2022.956421
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