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Rapid and label-free identification of single foodborne pathogens using microfluidic pore sensors

Foodborne pathogenic microorganisms have become major threats that endanger human life and health. The current technology cannot perform rapid screening of foodborne pathogenic bacteria, and fail to timely control food safety risks. Here, we develop a novel microfluidic sensor for real-time and labe...

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Autores principales: Yang, Tao, Luo, Zisheng, Wu, Ricardo A., Li, Li, Xu, Yanqun, Ding, Tian, Lin, Xingyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386262/
https://www.ncbi.nlm.nih.gov/pubmed/35990323
http://dx.doi.org/10.3389/fnut.2022.959317
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author Yang, Tao
Luo, Zisheng
Wu, Ricardo A.
Li, Li
Xu, Yanqun
Ding, Tian
Lin, Xingyu
author_facet Yang, Tao
Luo, Zisheng
Wu, Ricardo A.
Li, Li
Xu, Yanqun
Ding, Tian
Lin, Xingyu
author_sort Yang, Tao
collection PubMed
description Foodborne pathogenic microorganisms have become major threats that endanger human life and health. The current technology cannot perform rapid screening of foodborne pathogenic bacteria, and fail to timely control food safety risks. Here, we develop a novel microfluidic sensor for real-time and label-free bacteria classification at the single-cell level. Concretely, a low-aspect-ratio SiN micropore with PDMS coating was fabricated, which could significantly reduce the noise of the sensing system, and makes the microfluidic pore sensor sensitive to bacteria discrimination. The prepared SiN micropore equipped with the high temporal-spatial resolution was applied to observe bacterial translocation “events” and the current pulse signals could be obtained, which depend on the size, charge, and morphology of the target bacteria. According to the variation of the current pulse signals produced by different bacteria across the micropore, three common foodborne pathogens such as Salmonella enteric, Listeria monocytogenes, and Escherichia coli were identified. Due to convenience, rapidity, and accuracy, the label-free method we report here has great potential for the identification of diverse foodborne microorganisms at single-cell sensitivity.
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spelling pubmed-93862622022-08-19 Rapid and label-free identification of single foodborne pathogens using microfluidic pore sensors Yang, Tao Luo, Zisheng Wu, Ricardo A. Li, Li Xu, Yanqun Ding, Tian Lin, Xingyu Front Nutr Nutrition Foodborne pathogenic microorganisms have become major threats that endanger human life and health. The current technology cannot perform rapid screening of foodborne pathogenic bacteria, and fail to timely control food safety risks. Here, we develop a novel microfluidic sensor for real-time and label-free bacteria classification at the single-cell level. Concretely, a low-aspect-ratio SiN micropore with PDMS coating was fabricated, which could significantly reduce the noise of the sensing system, and makes the microfluidic pore sensor sensitive to bacteria discrimination. The prepared SiN micropore equipped with the high temporal-spatial resolution was applied to observe bacterial translocation “events” and the current pulse signals could be obtained, which depend on the size, charge, and morphology of the target bacteria. According to the variation of the current pulse signals produced by different bacteria across the micropore, three common foodborne pathogens such as Salmonella enteric, Listeria monocytogenes, and Escherichia coli were identified. Due to convenience, rapidity, and accuracy, the label-free method we report here has great potential for the identification of diverse foodborne microorganisms at single-cell sensitivity. Frontiers Media S.A. 2022-08-04 /pmc/articles/PMC9386262/ /pubmed/35990323 http://dx.doi.org/10.3389/fnut.2022.959317 Text en Copyright © 2022 Yang, Luo, Wu, Li, Xu, Ding and Lin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Yang, Tao
Luo, Zisheng
Wu, Ricardo A.
Li, Li
Xu, Yanqun
Ding, Tian
Lin, Xingyu
Rapid and label-free identification of single foodborne pathogens using microfluidic pore sensors
title Rapid and label-free identification of single foodborne pathogens using microfluidic pore sensors
title_full Rapid and label-free identification of single foodborne pathogens using microfluidic pore sensors
title_fullStr Rapid and label-free identification of single foodborne pathogens using microfluidic pore sensors
title_full_unstemmed Rapid and label-free identification of single foodborne pathogens using microfluidic pore sensors
title_short Rapid and label-free identification of single foodborne pathogens using microfluidic pore sensors
title_sort rapid and label-free identification of single foodborne pathogens using microfluidic pore sensors
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386262/
https://www.ncbi.nlm.nih.gov/pubmed/35990323
http://dx.doi.org/10.3389/fnut.2022.959317
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