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AMPKβ isoform expression patterns in various adipocyte models and in relation to body mass index
AMP-activated protein kinase (AMPK) activation is considered a useful strategy for the treatment of type 2 diabetes (T2D). It is unclear whether the expression and/or activity of AMPK in adipocytes is dysregulated in obesity. Also, the expression/activity pattern of AMPKβ isoforms, which are targets...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386264/ https://www.ncbi.nlm.nih.gov/pubmed/35991175 http://dx.doi.org/10.3389/fphys.2022.928964 |
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author | Kopietz, Franziska Degerman, Eva Göransson, Olga |
author_facet | Kopietz, Franziska Degerman, Eva Göransson, Olga |
author_sort | Kopietz, Franziska |
collection | PubMed |
description | AMP-activated protein kinase (AMPK) activation is considered a useful strategy for the treatment of type 2 diabetes (T2D). It is unclear whether the expression and/or activity of AMPK in adipocytes is dysregulated in obesity. Also, the expression/activity pattern of AMPKβ isoforms, which are targets for AMPK activators, in adipocytes remains elusive. In this study we show that the two AMPKβ isoforms make roughly equal contributions to AMPK activity in primary human and mouse adipocytes, whereas in cultured 3T3-L1 adipocytes of mouse origin and in primary rat adipocytes, β1-associated activity clearly dominates. Additionally, we found that obesity is not associated with changes in AMPK subunit expression or kinase activity in adipocytes isolated from subcutaneous adipose tissue from individuals with various BMI. |
format | Online Article Text |
id | pubmed-9386264 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93862642022-08-19 AMPKβ isoform expression patterns in various adipocyte models and in relation to body mass index Kopietz, Franziska Degerman, Eva Göransson, Olga Front Physiol Physiology AMP-activated protein kinase (AMPK) activation is considered a useful strategy for the treatment of type 2 diabetes (T2D). It is unclear whether the expression and/or activity of AMPK in adipocytes is dysregulated in obesity. Also, the expression/activity pattern of AMPKβ isoforms, which are targets for AMPK activators, in adipocytes remains elusive. In this study we show that the two AMPKβ isoforms make roughly equal contributions to AMPK activity in primary human and mouse adipocytes, whereas in cultured 3T3-L1 adipocytes of mouse origin and in primary rat adipocytes, β1-associated activity clearly dominates. Additionally, we found that obesity is not associated with changes in AMPK subunit expression or kinase activity in adipocytes isolated from subcutaneous adipose tissue from individuals with various BMI. Frontiers Media S.A. 2022-08-04 /pmc/articles/PMC9386264/ /pubmed/35991175 http://dx.doi.org/10.3389/fphys.2022.928964 Text en Copyright © 2022 Kopietz, Degerman and Göransson. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Kopietz, Franziska Degerman, Eva Göransson, Olga AMPKβ isoform expression patterns in various adipocyte models and in relation to body mass index |
title | AMPKβ isoform expression patterns in various adipocyte models and in relation to body mass index |
title_full | AMPKβ isoform expression patterns in various adipocyte models and in relation to body mass index |
title_fullStr | AMPKβ isoform expression patterns in various adipocyte models and in relation to body mass index |
title_full_unstemmed | AMPKβ isoform expression patterns in various adipocyte models and in relation to body mass index |
title_short | AMPKβ isoform expression patterns in various adipocyte models and in relation to body mass index |
title_sort | ampkβ isoform expression patterns in various adipocyte models and in relation to body mass index |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386264/ https://www.ncbi.nlm.nih.gov/pubmed/35991175 http://dx.doi.org/10.3389/fphys.2022.928964 |
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