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Reparative properties of human glioblastoma cells after single exposure to a wide range of X-ray doses

Radiation therapy induces double-stranded DNA breaks in tumor cells, which leads to their death. A fraction of glioblastoma cells repair such breaks and reinitiate tumor growth. It was necessary to identify the relationship between high radiation doses and the proliferative activity of glioblastoma...

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Autores principales: Pavlova, Galina, Belyashova, Alexandra, Savchenko, Ekaterina, Panteleev, Dmitri, Shamadykova, Dzhirgala, Nikolaeva, Anna, Pavlova, Svetlana, Revishchin, Alexander, Golbin, Denis, Potapov, Alexander, Antipina, Natalia, Golanov, Andrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386365/
https://www.ncbi.nlm.nih.gov/pubmed/35992802
http://dx.doi.org/10.3389/fonc.2022.912741
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author Pavlova, Galina
Belyashova, Alexandra
Savchenko, Ekaterina
Panteleev, Dmitri
Shamadykova, Dzhirgala
Nikolaeva, Anna
Pavlova, Svetlana
Revishchin, Alexander
Golbin, Denis
Potapov, Alexander
Antipina, Natalia
Golanov, Andrey
author_facet Pavlova, Galina
Belyashova, Alexandra
Savchenko, Ekaterina
Panteleev, Dmitri
Shamadykova, Dzhirgala
Nikolaeva, Anna
Pavlova, Svetlana
Revishchin, Alexander
Golbin, Denis
Potapov, Alexander
Antipina, Natalia
Golanov, Andrey
author_sort Pavlova, Galina
collection PubMed
description Radiation therapy induces double-stranded DNA breaks in tumor cells, which leads to their death. A fraction of glioblastoma cells repair such breaks and reinitiate tumor growth. It was necessary to identify the relationship between high radiation doses and the proliferative activity of glioblastoma cells, and to evaluate the contribution of DNA repair pathways, homologous recombination (HR), and nonhomologous end joining (NHEJ) to tumor-cell recovery. We demonstrated that the GO1 culture derived from glioblastoma cells from Patient G, who had previously been irradiated, proved to be less sensitive to radiation than the Sus\fP2 glioblastoma culture was from Patient S, who had not been exposed to radiation before. GO1 cell proliferation decreased with radiation dose, and MTT decreased to 35% after a single exposure to 125 Gγ. The proliferative potential of glioblastoma culture Sus\fP2 decreased to 35% after exposure to 5 Gγ. At low radiation doses, cell proliferation and the expression of RAD51 were decreased; at high doses, cell proliferation was correlated with Ku70 protein expression. Therefore, HR and NHEJ are involved in DNA break repair after exposure to different radiation doses. Low doses induce HR, while higher doses induce the faster but less accurate NHEJ pathway of double-stranded DNA break repair.
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spelling pubmed-93863652022-08-19 Reparative properties of human glioblastoma cells after single exposure to a wide range of X-ray doses Pavlova, Galina Belyashova, Alexandra Savchenko, Ekaterina Panteleev, Dmitri Shamadykova, Dzhirgala Nikolaeva, Anna Pavlova, Svetlana Revishchin, Alexander Golbin, Denis Potapov, Alexander Antipina, Natalia Golanov, Andrey Front Oncol Oncology Radiation therapy induces double-stranded DNA breaks in tumor cells, which leads to their death. A fraction of glioblastoma cells repair such breaks and reinitiate tumor growth. It was necessary to identify the relationship between high radiation doses and the proliferative activity of glioblastoma cells, and to evaluate the contribution of DNA repair pathways, homologous recombination (HR), and nonhomologous end joining (NHEJ) to tumor-cell recovery. We demonstrated that the GO1 culture derived from glioblastoma cells from Patient G, who had previously been irradiated, proved to be less sensitive to radiation than the Sus\fP2 glioblastoma culture was from Patient S, who had not been exposed to radiation before. GO1 cell proliferation decreased with radiation dose, and MTT decreased to 35% after a single exposure to 125 Gγ. The proliferative potential of glioblastoma culture Sus\fP2 decreased to 35% after exposure to 5 Gγ. At low radiation doses, cell proliferation and the expression of RAD51 were decreased; at high doses, cell proliferation was correlated with Ku70 protein expression. Therefore, HR and NHEJ are involved in DNA break repair after exposure to different radiation doses. Low doses induce HR, while higher doses induce the faster but less accurate NHEJ pathway of double-stranded DNA break repair. Frontiers Media S.A. 2022-08-04 /pmc/articles/PMC9386365/ /pubmed/35992802 http://dx.doi.org/10.3389/fonc.2022.912741 Text en Copyright © 2022 Pavlova, Belyashova, Savchenko, Panteleev, Shamadykova, Nikolaeva, Pavlova, Revishchin, Golbin, Potapov, Antipina and Golanov https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Pavlova, Galina
Belyashova, Alexandra
Savchenko, Ekaterina
Panteleev, Dmitri
Shamadykova, Dzhirgala
Nikolaeva, Anna
Pavlova, Svetlana
Revishchin, Alexander
Golbin, Denis
Potapov, Alexander
Antipina, Natalia
Golanov, Andrey
Reparative properties of human glioblastoma cells after single exposure to a wide range of X-ray doses
title Reparative properties of human glioblastoma cells after single exposure to a wide range of X-ray doses
title_full Reparative properties of human glioblastoma cells after single exposure to a wide range of X-ray doses
title_fullStr Reparative properties of human glioblastoma cells after single exposure to a wide range of X-ray doses
title_full_unstemmed Reparative properties of human glioblastoma cells after single exposure to a wide range of X-ray doses
title_short Reparative properties of human glioblastoma cells after single exposure to a wide range of X-ray doses
title_sort reparative properties of human glioblastoma cells after single exposure to a wide range of x-ray doses
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386365/
https://www.ncbi.nlm.nih.gov/pubmed/35992802
http://dx.doi.org/10.3389/fonc.2022.912741
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