Hairy root transformation system as a tool for CRISPR/Cas9-directed genome editing in oilseed rape (Brassica napus)

Our study examined the mutation efficiency of the CRISPR/Cas9 method for tryptophan aminotransferase BnaTAA1 genes involved in the auxin biosynthesis pathway. We made nine CRISPR/Cas9 constructs with various promoters driving the expression of a Cas9 from Staphylococcus aureus (SaCas9) or a plant-co...

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Detalles Bibliográficos
Autores principales: Jedličková, Veronika, Mácová, Kateřina, Štefková, Marie, Butula, Jan, Staveníková, Jana, Sedláček, Marek, Robert, Hélène S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386449/
https://www.ncbi.nlm.nih.gov/pubmed/35991410
http://dx.doi.org/10.3389/fpls.2022.919290

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386449/
https://www.ncbi.nlm.nih.gov/pubmed/35991410
http://dx.doi.org/10.3389/fpls.2022.919290

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