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Hairy root transformation system as a tool for CRISPR/Cas9-directed genome editing in oilseed rape (Brassica napus)
Our study examined the mutation efficiency of the CRISPR/Cas9 method for tryptophan aminotransferase BnaTAA1 genes involved in the auxin biosynthesis pathway. We made nine CRISPR/Cas9 constructs with various promoters driving the expression of a Cas9 from Staphylococcus aureus (SaCas9) or a plant-co...
Autores principales: | Jedličková, Veronika, Mácová, Kateřina, Štefková, Marie, Butula, Jan, Staveníková, Jana, Sedláček, Marek, Robert, Hélène S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386449/ https://www.ncbi.nlm.nih.gov/pubmed/35991410 http://dx.doi.org/10.3389/fpls.2022.919290 |
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