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CD44 expressed by myeloid cells promotes glioma invasion

Glioblastoma multiforme (GBM) is one of the most common and malignant brain tumors in adulthood with a median survival of only 15 months. This poor prognosis is related to GBM’s ability to extensively infiltrate the surrounding brain parenchyma resulting in diffuse spread of neoplastic cells in the...

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Autores principales: Ivanova, Ekaterina L., Costa, Barbara, Eisemann, Tanja, Lohr, Sabrina, Boskovic, Pavle, Eichwald, Viktoria, Meckler, Jasmin, Jugold, Manfred, Orian-Rousseau, Veronique, Peterziel, Heike, Angel, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386454/
https://www.ncbi.nlm.nih.gov/pubmed/35992852
http://dx.doi.org/10.3389/fonc.2022.969787
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author Ivanova, Ekaterina L.
Costa, Barbara
Eisemann, Tanja
Lohr, Sabrina
Boskovic, Pavle
Eichwald, Viktoria
Meckler, Jasmin
Jugold, Manfred
Orian-Rousseau, Veronique
Peterziel, Heike
Angel, Peter
author_facet Ivanova, Ekaterina L.
Costa, Barbara
Eisemann, Tanja
Lohr, Sabrina
Boskovic, Pavle
Eichwald, Viktoria
Meckler, Jasmin
Jugold, Manfred
Orian-Rousseau, Veronique
Peterziel, Heike
Angel, Peter
author_sort Ivanova, Ekaterina L.
collection PubMed
description Glioblastoma multiforme (GBM) is one of the most common and malignant brain tumors in adulthood with a median survival of only 15 months. This poor prognosis is related to GBM’s ability to extensively infiltrate the surrounding brain parenchyma resulting in diffuse spread of neoplastic cells in the brain, responsible for high rate of recurrence. CD44 (Cluster of Differentiation 44) is a transmembrane protein, overexpressed in multiple cancer types, including gliomas, and implicated in cell motility, proliferation and angiogenesis. Multiple studies have investigated the role of CD44 in GBM cells and have highlighted a link between tumor malignancy and CD44 expression. However up to date, little is known of the role of CD44 on cells from the tumor microenvironment (TME). Here, we have investigated a potential role of CD44 in the TME in regards to GBM invasiveness. Using an ex-vivo organotypic brain slice invasion assay, we show that absence of CD44 from the TME impairs the ability of glioma cells to invade the surrounding brain parenchyma. By deleting CD44 in the astrocytic, endothelial and myeloid compartments, we show that it is specifically CD44 expression in myeloid cells that is responsible for the observed phenotype. Combining in vivo studies in cell-specific knock-out mice and in vitro analyses on primary microglia we demonstrate that myeloid CD44 is implicated in Toll Like Receptor 2 signaling and is a major regulator of Matrix metalloproteinase 9 expression.
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spelling pubmed-93864542022-08-19 CD44 expressed by myeloid cells promotes glioma invasion Ivanova, Ekaterina L. Costa, Barbara Eisemann, Tanja Lohr, Sabrina Boskovic, Pavle Eichwald, Viktoria Meckler, Jasmin Jugold, Manfred Orian-Rousseau, Veronique Peterziel, Heike Angel, Peter Front Oncol Oncology Glioblastoma multiforme (GBM) is one of the most common and malignant brain tumors in adulthood with a median survival of only 15 months. This poor prognosis is related to GBM’s ability to extensively infiltrate the surrounding brain parenchyma resulting in diffuse spread of neoplastic cells in the brain, responsible for high rate of recurrence. CD44 (Cluster of Differentiation 44) is a transmembrane protein, overexpressed in multiple cancer types, including gliomas, and implicated in cell motility, proliferation and angiogenesis. Multiple studies have investigated the role of CD44 in GBM cells and have highlighted a link between tumor malignancy and CD44 expression. However up to date, little is known of the role of CD44 on cells from the tumor microenvironment (TME). Here, we have investigated a potential role of CD44 in the TME in regards to GBM invasiveness. Using an ex-vivo organotypic brain slice invasion assay, we show that absence of CD44 from the TME impairs the ability of glioma cells to invade the surrounding brain parenchyma. By deleting CD44 in the astrocytic, endothelial and myeloid compartments, we show that it is specifically CD44 expression in myeloid cells that is responsible for the observed phenotype. Combining in vivo studies in cell-specific knock-out mice and in vitro analyses on primary microglia we demonstrate that myeloid CD44 is implicated in Toll Like Receptor 2 signaling and is a major regulator of Matrix metalloproteinase 9 expression. Frontiers Media S.A. 2022-08-04 /pmc/articles/PMC9386454/ /pubmed/35992852 http://dx.doi.org/10.3389/fonc.2022.969787 Text en Copyright © 2022 Ivanova, Costa, Eisemann, Lohr, Boskovic, Eichwald, Meckler, Jugold, Orian-Rousseau, Peterziel and Angel https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Ivanova, Ekaterina L.
Costa, Barbara
Eisemann, Tanja
Lohr, Sabrina
Boskovic, Pavle
Eichwald, Viktoria
Meckler, Jasmin
Jugold, Manfred
Orian-Rousseau, Veronique
Peterziel, Heike
Angel, Peter
CD44 expressed by myeloid cells promotes glioma invasion
title CD44 expressed by myeloid cells promotes glioma invasion
title_full CD44 expressed by myeloid cells promotes glioma invasion
title_fullStr CD44 expressed by myeloid cells promotes glioma invasion
title_full_unstemmed CD44 expressed by myeloid cells promotes glioma invasion
title_short CD44 expressed by myeloid cells promotes glioma invasion
title_sort cd44 expressed by myeloid cells promotes glioma invasion
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386454/
https://www.ncbi.nlm.nih.gov/pubmed/35992852
http://dx.doi.org/10.3389/fonc.2022.969787
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