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Development of the engineered “glanded plant and glandless seed” cotton

After fiber, cottonseed is the second most important by-product of cotton production. However, high concentrations of toxic free gossypol deposited in the glands of the cottonseed greatly hamper its effective usage as food or feed. Here, we developed a cotton line with edible cottonseed by specifica...

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Detalles Bibliográficos
Autores principales: Gao, Wenhao, Zhu, Xiefei, Ding, Lingyun, Xu, Biyu, Gao, Yang, Cheng, Yu, Dai, Fan, Liu, Bingliang, Si, Zhanfeng, Fang, Lei, Guan, Xueying, Zhu, Shuijin, Zhang, Tianzhen, Hu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386459/
https://www.ncbi.nlm.nih.gov/pubmed/35992508
http://dx.doi.org/10.1016/j.fochms.2022.100130
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author Gao, Wenhao
Zhu, Xiefei
Ding, Lingyun
Xu, Biyu
Gao, Yang
Cheng, Yu
Dai, Fan
Liu, Bingliang
Si, Zhanfeng
Fang, Lei
Guan, Xueying
Zhu, Shuijin
Zhang, Tianzhen
Hu, Yan
author_facet Gao, Wenhao
Zhu, Xiefei
Ding, Lingyun
Xu, Biyu
Gao, Yang
Cheng, Yu
Dai, Fan
Liu, Bingliang
Si, Zhanfeng
Fang, Lei
Guan, Xueying
Zhu, Shuijin
Zhang, Tianzhen
Hu, Yan
author_sort Gao, Wenhao
collection PubMed
description After fiber, cottonseed is the second most important by-product of cotton production. However, high concentrations of toxic free gossypol deposited in the glands of the cottonseed greatly hamper its effective usage as food or feed. Here, we developed a cotton line with edible cottonseed by specifically silencing the endogenous expression of GoPGF in the seeds, which led to a glandless phenotype with an ultra-low gossypol content in the seeds and nearly normal gossypol in other parts of the plants. This engineered cotton maintains normal resistance to insect pests, but the gossypol content in the seeds dropped by 98%, and thus, it can be consumed directly as food. The trait of a low gossypol content in the cottonseeds was stable and heritable, while the protein, oil content, and fiber yield or quality were nearly unchanged compared to the transgenic receptor W0. In addition, comparative transcriptome analysis showed that down-regulated genes in the ovules of the glandless cotton were enriched in terpenoid biosynthesis, indicating the underlying relationship between gland formation and gossypol biosynthesis. These results pave the way for the comprehensive utilization of cotton as a fiber, oil, and feed crop in the future.
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spelling pubmed-93864592022-08-19 Development of the engineered “glanded plant and glandless seed” cotton Gao, Wenhao Zhu, Xiefei Ding, Lingyun Xu, Biyu Gao, Yang Cheng, Yu Dai, Fan Liu, Bingliang Si, Zhanfeng Fang, Lei Guan, Xueying Zhu, Shuijin Zhang, Tianzhen Hu, Yan Food Chem (Oxf) Research Article After fiber, cottonseed is the second most important by-product of cotton production. However, high concentrations of toxic free gossypol deposited in the glands of the cottonseed greatly hamper its effective usage as food or feed. Here, we developed a cotton line with edible cottonseed by specifically silencing the endogenous expression of GoPGF in the seeds, which led to a glandless phenotype with an ultra-low gossypol content in the seeds and nearly normal gossypol in other parts of the plants. This engineered cotton maintains normal resistance to insect pests, but the gossypol content in the seeds dropped by 98%, and thus, it can be consumed directly as food. The trait of a low gossypol content in the cottonseeds was stable and heritable, while the protein, oil content, and fiber yield or quality were nearly unchanged compared to the transgenic receptor W0. In addition, comparative transcriptome analysis showed that down-regulated genes in the ovules of the glandless cotton were enriched in terpenoid biosynthesis, indicating the underlying relationship between gland formation and gossypol biosynthesis. These results pave the way for the comprehensive utilization of cotton as a fiber, oil, and feed crop in the future. Elsevier 2022-08-09 /pmc/articles/PMC9386459/ /pubmed/35992508 http://dx.doi.org/10.1016/j.fochms.2022.100130 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Gao, Wenhao
Zhu, Xiefei
Ding, Lingyun
Xu, Biyu
Gao, Yang
Cheng, Yu
Dai, Fan
Liu, Bingliang
Si, Zhanfeng
Fang, Lei
Guan, Xueying
Zhu, Shuijin
Zhang, Tianzhen
Hu, Yan
Development of the engineered “glanded plant and glandless seed” cotton
title Development of the engineered “glanded plant and glandless seed” cotton
title_full Development of the engineered “glanded plant and glandless seed” cotton
title_fullStr Development of the engineered “glanded plant and glandless seed” cotton
title_full_unstemmed Development of the engineered “glanded plant and glandless seed” cotton
title_short Development of the engineered “glanded plant and glandless seed” cotton
title_sort development of the engineered “glanded plant and glandless seed” cotton
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386459/
https://www.ncbi.nlm.nih.gov/pubmed/35992508
http://dx.doi.org/10.1016/j.fochms.2022.100130
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