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Application of Quantum Cascade Laser-Infrared Spectroscopy and Chemometrics for In-Line Discrimination of Coeluting Proteins from Preparative Size Exclusion Chromatography

[Image: see text] An external-cavity quantum cascade laser (EC-QCL)-based flow-through mid-infrared (IR) spectrometer was placed in line with a preparative size exclusion chromatography system to demonstrate real-time analysis of protein elutions with strongly overlapping chromatographic peaks. Two...

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Detalles Bibliográficos
Autores principales:	Akhgar, Christopher K., Ebner, Julian, Alcaraz, Mirta R., Kopp, Julian, Goicoechea, Héctor, Spadiut, Oliver, Schwaighofer, Andreas, Lendl, Bernhard
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2022
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386682/ https://www.ncbi.nlm.nih.gov/pubmed/35926134 http://dx.doi.org/10.1021/acs.analchem.2c01542

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