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Valerian essential oil for treating insomnia via the serotonergic synapse pathway

Valerian volatile oil can be used in the treatment of insomnia; however, the active components and mechanisms of action are currently unclear. Therefore, we used transcriptome sequencing and weight coefficient network pharmacology to predict the effective components and mechanism of action of valeri...

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Autores principales: Wang, Wenfei, Wang, Yichun, Guo, Qiuting, Li, Huiting, Wang, Zhaoqiang, Li, Jia, Li, Taotao, Tang, Tiantian, Wang, Yujiao, Jia, Yanzhuo, Wang, Yao, Zou, Junbo, Shi, Yajun, Guo, Dongyan, Yang, Ming, Zhang, Xiaofei, Sun, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9387164/
https://www.ncbi.nlm.nih.gov/pubmed/35990355
http://dx.doi.org/10.3389/fnut.2022.927434
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author Wang, Wenfei
Wang, Yichun
Guo, Qiuting
Li, Huiting
Wang, Zhaoqiang
Li, Jia
Li, Taotao
Tang, Tiantian
Wang, Yujiao
Jia, Yanzhuo
Wang, Yao
Zou, Junbo
Shi, Yajun
Guo, Dongyan
Yang, Ming
Zhang, Xiaofei
Sun, Jing
author_facet Wang, Wenfei
Wang, Yichun
Guo, Qiuting
Li, Huiting
Wang, Zhaoqiang
Li, Jia
Li, Taotao
Tang, Tiantian
Wang, Yujiao
Jia, Yanzhuo
Wang, Yao
Zou, Junbo
Shi, Yajun
Guo, Dongyan
Yang, Ming
Zhang, Xiaofei
Sun, Jing
author_sort Wang, Wenfei
collection PubMed
description Valerian volatile oil can be used in the treatment of insomnia; however, the active components and mechanisms of action are currently unclear. Therefore, we used transcriptome sequencing and weight coefficient network pharmacology to predict the effective components and mechanism of action of valerian volatile oil in an insomnia model induced by intraperitoneal injection of para-Chlorophenylalanine (PCPA) in SD rats. Valerian essential oil was given orally for treatment and the contents of 5-hydroxytryptamine receptor 1 A (5-HT1AR), γ-aminobutyric acid (GABA), cyclic adenosine monophosphate (cAMP), and protein kinase A (PKA) in the hippocampus of rats in each group were detected by enzyme-linked immunosorbent assay (ELISA), western blot, Polymerase Chain Reaction (PCR), and immunohistochemistry. The results showed that after treatment with valerian essential oil, insomnia rats showed significantly prolonged sleep duration and alleviated insomnia-induced tension and anxiety. Regarding the mechanism of action, we believe that caryophyllene in valerian essential oil upregulates the 5-HT1AR receptor to improve the activity or affinity of the central transmitter 5-HT, increase the release of 5-HT, couple 5-HT with a G protein coupled receptor, convert adenosine triphosphate (ATP) into cAMP (catalyzed by ADCY5), and then directly regulate the downstream pathway. Following pathway activation, we propose that the core gene protein kinase PKA activates the serotonergic synapse signal pathway to increase the expression of 5-HT and GABA, thus improving insomnia symptoms and alleviating anxiety. This study provides a theoretical basis for the application of valerian volatile oil in health food.
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spelling pubmed-93871642022-08-19 Valerian essential oil for treating insomnia via the serotonergic synapse pathway Wang, Wenfei Wang, Yichun Guo, Qiuting Li, Huiting Wang, Zhaoqiang Li, Jia Li, Taotao Tang, Tiantian Wang, Yujiao Jia, Yanzhuo Wang, Yao Zou, Junbo Shi, Yajun Guo, Dongyan Yang, Ming Zhang, Xiaofei Sun, Jing Front Nutr Nutrition Valerian volatile oil can be used in the treatment of insomnia; however, the active components and mechanisms of action are currently unclear. Therefore, we used transcriptome sequencing and weight coefficient network pharmacology to predict the effective components and mechanism of action of valerian volatile oil in an insomnia model induced by intraperitoneal injection of para-Chlorophenylalanine (PCPA) in SD rats. Valerian essential oil was given orally for treatment and the contents of 5-hydroxytryptamine receptor 1 A (5-HT1AR), γ-aminobutyric acid (GABA), cyclic adenosine monophosphate (cAMP), and protein kinase A (PKA) in the hippocampus of rats in each group were detected by enzyme-linked immunosorbent assay (ELISA), western blot, Polymerase Chain Reaction (PCR), and immunohistochemistry. The results showed that after treatment with valerian essential oil, insomnia rats showed significantly prolonged sleep duration and alleviated insomnia-induced tension and anxiety. Regarding the mechanism of action, we believe that caryophyllene in valerian essential oil upregulates the 5-HT1AR receptor to improve the activity or affinity of the central transmitter 5-HT, increase the release of 5-HT, couple 5-HT with a G protein coupled receptor, convert adenosine triphosphate (ATP) into cAMP (catalyzed by ADCY5), and then directly regulate the downstream pathway. Following pathway activation, we propose that the core gene protein kinase PKA activates the serotonergic synapse signal pathway to increase the expression of 5-HT and GABA, thus improving insomnia symptoms and alleviating anxiety. This study provides a theoretical basis for the application of valerian volatile oil in health food. Frontiers Media S.A. 2022-07-28 /pmc/articles/PMC9387164/ /pubmed/35990355 http://dx.doi.org/10.3389/fnut.2022.927434 Text en Copyright © 2022 Wang, Wang, Guo, Li, Wang, Li, Li, Tang, Wang, Jia, Wang, Zou, Shi, Guo, Yang, Zhang and Sun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Wang, Wenfei
Wang, Yichun
Guo, Qiuting
Li, Huiting
Wang, Zhaoqiang
Li, Jia
Li, Taotao
Tang, Tiantian
Wang, Yujiao
Jia, Yanzhuo
Wang, Yao
Zou, Junbo
Shi, Yajun
Guo, Dongyan
Yang, Ming
Zhang, Xiaofei
Sun, Jing
Valerian essential oil for treating insomnia via the serotonergic synapse pathway
title Valerian essential oil for treating insomnia via the serotonergic synapse pathway
title_full Valerian essential oil for treating insomnia via the serotonergic synapse pathway
title_fullStr Valerian essential oil for treating insomnia via the serotonergic synapse pathway
title_full_unstemmed Valerian essential oil for treating insomnia via the serotonergic synapse pathway
title_short Valerian essential oil for treating insomnia via the serotonergic synapse pathway
title_sort valerian essential oil for treating insomnia via the serotonergic synapse pathway
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9387164/
https://www.ncbi.nlm.nih.gov/pubmed/35990355
http://dx.doi.org/10.3389/fnut.2022.927434
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