Cargando…

ESR2 expression in subcutaneous adipose tissue is related to body fat distribution in women, and knockdown impairs preadipocyte differentiation

Oestrogen receptor 2 (ESR2) expression has been shown to be higher in subcutaneous adipose tissue (SAT) from postmenopausal compared to premenopausal women. The functional significance of altered ESR2 expression is not fully known. This study investigates the role of ESR2 for adipose tissue lipid an...

Descripción completa

Detalles Bibliográficos
Autores principales: Ahmed, Fozia, Hetty, Susanne, Vranic, Milica, Fanni, Giovanni, Kullberg, Joel, Pereira, Maria João, Eriksson, Jan W
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9387337/
https://www.ncbi.nlm.nih.gov/pubmed/35856485
http://dx.doi.org/10.1080/21623945.2022.2102116
_version_ 1784769999663005696
author Ahmed, Fozia
Hetty, Susanne
Vranic, Milica
Fanni, Giovanni
Kullberg, Joel
Pereira, Maria João
Eriksson, Jan W
author_facet Ahmed, Fozia
Hetty, Susanne
Vranic, Milica
Fanni, Giovanni
Kullberg, Joel
Pereira, Maria João
Eriksson, Jan W
author_sort Ahmed, Fozia
collection PubMed
description Oestrogen receptor 2 (ESR2) expression has been shown to be higher in subcutaneous adipose tissue (SAT) from postmenopausal compared to premenopausal women. The functional significance of altered ESR2 expression is not fully known. This study investigates the role of ESR2 for adipose tissue lipid and glucose metabolism. SAT biopsies were obtained from 44 female subjects with or without T2D. Gene expression of ESR2 and markers of adipose function and metabolism was assessed. ESR2 knockdown was performed using CRISPR/Cas9 in preadipocytes isolated from SAT of females, and differentiation rate, lipid storage, and glucose uptake were measured. ESR2 expression was inversely correlated with measures of central obesity and expression of some fatty acid oxidation markers, and positively correlated with lipid storage and glucose transport markers. Differentiation was reduced in ESR2 knockdown preadipocytes. This corresponded to reduced expression of markers of differentiation and lipogenesis. Glucose uptake was reduced in knockdown adipocytes. Our results indicate that ESR2 deficiency in women is associated with visceral adiposity and impaired subcutaneous adipocyte differentiation as well as glucose and lipid utilization. High ESR2 expression, as seen after menopause, could be a contributing factor to SAT expansion. This may support a possible target to promote a healthy obesity phenotype.
format Online
Article
Text
id pubmed-9387337
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-93873372022-08-19 ESR2 expression in subcutaneous adipose tissue is related to body fat distribution in women, and knockdown impairs preadipocyte differentiation Ahmed, Fozia Hetty, Susanne Vranic, Milica Fanni, Giovanni Kullberg, Joel Pereira, Maria João Eriksson, Jan W Adipocyte Research Paper Oestrogen receptor 2 (ESR2) expression has been shown to be higher in subcutaneous adipose tissue (SAT) from postmenopausal compared to premenopausal women. The functional significance of altered ESR2 expression is not fully known. This study investigates the role of ESR2 for adipose tissue lipid and glucose metabolism. SAT biopsies were obtained from 44 female subjects with or without T2D. Gene expression of ESR2 and markers of adipose function and metabolism was assessed. ESR2 knockdown was performed using CRISPR/Cas9 in preadipocytes isolated from SAT of females, and differentiation rate, lipid storage, and glucose uptake were measured. ESR2 expression was inversely correlated with measures of central obesity and expression of some fatty acid oxidation markers, and positively correlated with lipid storage and glucose transport markers. Differentiation was reduced in ESR2 knockdown preadipocytes. This corresponded to reduced expression of markers of differentiation and lipogenesis. Glucose uptake was reduced in knockdown adipocytes. Our results indicate that ESR2 deficiency in women is associated with visceral adiposity and impaired subcutaneous adipocyte differentiation as well as glucose and lipid utilization. High ESR2 expression, as seen after menopause, could be a contributing factor to SAT expansion. This may support a possible target to promote a healthy obesity phenotype. Taylor & Francis 2022-08-17 /pmc/articles/PMC9387337/ /pubmed/35856485 http://dx.doi.org/10.1080/21623945.2022.2102116 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Ahmed, Fozia
Hetty, Susanne
Vranic, Milica
Fanni, Giovanni
Kullberg, Joel
Pereira, Maria João
Eriksson, Jan W
ESR2 expression in subcutaneous adipose tissue is related to body fat distribution in women, and knockdown impairs preadipocyte differentiation
title ESR2 expression in subcutaneous adipose tissue is related to body fat distribution in women, and knockdown impairs preadipocyte differentiation
title_full ESR2 expression in subcutaneous adipose tissue is related to body fat distribution in women, and knockdown impairs preadipocyte differentiation
title_fullStr ESR2 expression in subcutaneous adipose tissue is related to body fat distribution in women, and knockdown impairs preadipocyte differentiation
title_full_unstemmed ESR2 expression in subcutaneous adipose tissue is related to body fat distribution in women, and knockdown impairs preadipocyte differentiation
title_short ESR2 expression in subcutaneous adipose tissue is related to body fat distribution in women, and knockdown impairs preadipocyte differentiation
title_sort esr2 expression in subcutaneous adipose tissue is related to body fat distribution in women, and knockdown impairs preadipocyte differentiation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9387337/
https://www.ncbi.nlm.nih.gov/pubmed/35856485
http://dx.doi.org/10.1080/21623945.2022.2102116
work_keys_str_mv AT ahmedfozia esr2expressioninsubcutaneousadiposetissueisrelatedtobodyfatdistributioninwomenandknockdownimpairspreadipocytedifferentiation
AT hettysusanne esr2expressioninsubcutaneousadiposetissueisrelatedtobodyfatdistributioninwomenandknockdownimpairspreadipocytedifferentiation
AT vranicmilica esr2expressioninsubcutaneousadiposetissueisrelatedtobodyfatdistributioninwomenandknockdownimpairspreadipocytedifferentiation
AT fannigiovanni esr2expressioninsubcutaneousadiposetissueisrelatedtobodyfatdistributioninwomenandknockdownimpairspreadipocytedifferentiation
AT kullbergjoel esr2expressioninsubcutaneousadiposetissueisrelatedtobodyfatdistributioninwomenandknockdownimpairspreadipocytedifferentiation
AT pereiramariajoao esr2expressioninsubcutaneousadiposetissueisrelatedtobodyfatdistributioninwomenandknockdownimpairspreadipocytedifferentiation
AT erikssonjanw esr2expressioninsubcutaneousadiposetissueisrelatedtobodyfatdistributioninwomenandknockdownimpairspreadipocytedifferentiation