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Unwrapping Wrap-around in Gas (or Liquid) Chromatographic Cyclic Ion Mobility–Mass Spectrometry
[Image: see text] Gas chromatography multiplexed with cyclic ion mobility mass spectrometry is a comprehensive two-dimensional separation technique that can resolve compounds that would otherwise coelute in a single-dimension separation. The cyclic geometry of the ion mobility cell enables ions to t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9387526/ https://www.ncbi.nlm.nih.gov/pubmed/35913896 http://dx.doi.org/10.1021/acs.analchem.2c02351 |
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author | Breen, Jake Hashemihedeshi, Mahin Amiri, Roshanak Dorman, Frank L. Jobst, Karl J. |
author_facet | Breen, Jake Hashemihedeshi, Mahin Amiri, Roshanak Dorman, Frank L. Jobst, Karl J. |
author_sort | Breen, Jake |
collection | PubMed |
description | [Image: see text] Gas chromatography multiplexed with cyclic ion mobility mass spectrometry is a comprehensive two-dimensional separation technique that can resolve compounds that would otherwise coelute in a single-dimension separation. The cyclic geometry of the ion mobility cell enables ions to travel multiple passes, increasing their drift times to the detector and relative separation. However, the quality of the separation may be obfuscated when “wrap-around” occurs, during which speedier ions catch up with slower ion populations when allowed to travel through more than one pass. Consequently, cyclic ion mobility is incorrectly perceived as a targeted approach that requires preselection of ions prior to separation. The present study demonstrates that “wrap-around” can be mitigated by comparing drift times measured during single- and multipass experiments and extrapolating the number of passes experienced by each ion. This straightforward calculation results in the “unwrapping” of cyclic ion mobility data so that the experiments can be interpreted in a nontargeted way while reaping the benefit of peak capacities that rival those achieved using other comprehensive two-dimensional separations. |
format | Online Article Text |
id | pubmed-9387526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-93875262023-08-01 Unwrapping Wrap-around in Gas (or Liquid) Chromatographic Cyclic Ion Mobility–Mass Spectrometry Breen, Jake Hashemihedeshi, Mahin Amiri, Roshanak Dorman, Frank L. Jobst, Karl J. Anal Chem [Image: see text] Gas chromatography multiplexed with cyclic ion mobility mass spectrometry is a comprehensive two-dimensional separation technique that can resolve compounds that would otherwise coelute in a single-dimension separation. The cyclic geometry of the ion mobility cell enables ions to travel multiple passes, increasing their drift times to the detector and relative separation. However, the quality of the separation may be obfuscated when “wrap-around” occurs, during which speedier ions catch up with slower ion populations when allowed to travel through more than one pass. Consequently, cyclic ion mobility is incorrectly perceived as a targeted approach that requires preselection of ions prior to separation. The present study demonstrates that “wrap-around” can be mitigated by comparing drift times measured during single- and multipass experiments and extrapolating the number of passes experienced by each ion. This straightforward calculation results in the “unwrapping” of cyclic ion mobility data so that the experiments can be interpreted in a nontargeted way while reaping the benefit of peak capacities that rival those achieved using other comprehensive two-dimensional separations. American Chemical Society 2022-08-01 2022-08-16 /pmc/articles/PMC9387526/ /pubmed/35913896 http://dx.doi.org/10.1021/acs.analchem.2c02351 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Breen, Jake Hashemihedeshi, Mahin Amiri, Roshanak Dorman, Frank L. Jobst, Karl J. Unwrapping Wrap-around in Gas (or Liquid) Chromatographic Cyclic Ion Mobility–Mass Spectrometry |
title | Unwrapping
Wrap-around in Gas (or Liquid) Chromatographic
Cyclic Ion Mobility–Mass Spectrometry |
title_full | Unwrapping
Wrap-around in Gas (or Liquid) Chromatographic
Cyclic Ion Mobility–Mass Spectrometry |
title_fullStr | Unwrapping
Wrap-around in Gas (or Liquid) Chromatographic
Cyclic Ion Mobility–Mass Spectrometry |
title_full_unstemmed | Unwrapping
Wrap-around in Gas (or Liquid) Chromatographic
Cyclic Ion Mobility–Mass Spectrometry |
title_short | Unwrapping
Wrap-around in Gas (or Liquid) Chromatographic
Cyclic Ion Mobility–Mass Spectrometry |
title_sort | unwrapping
wrap-around in gas (or liquid) chromatographic
cyclic ion mobility–mass spectrometry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9387526/ https://www.ncbi.nlm.nih.gov/pubmed/35913896 http://dx.doi.org/10.1021/acs.analchem.2c02351 |
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