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Optimum time for hand pollination in yam (Dioscorea spp.)

Hand pollination success rate is low in yam (Dioscorea spp.), due partly to suboptimal weather conditions. Thus, determining the most suitable time for pollination could improve the pollination success in yam breeding programs. We performed continuous hand pollination within flowering windows of D....

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Autores principales: Mondo, Jean M., Agre, Paterne A., Asiedu, Robert, Akoroda, Malachy O., Asfaw, Asrat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9387836/
https://www.ncbi.nlm.nih.gov/pubmed/35980958
http://dx.doi.org/10.1371/journal.pone.0269670
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author Mondo, Jean M.
Agre, Paterne A.
Asiedu, Robert
Akoroda, Malachy O.
Asfaw, Asrat
author_facet Mondo, Jean M.
Agre, Paterne A.
Asiedu, Robert
Akoroda, Malachy O.
Asfaw, Asrat
author_sort Mondo, Jean M.
collection PubMed
description Hand pollination success rate is low in yam (Dioscorea spp.), due partly to suboptimal weather conditions. Thus, determining the most suitable time for pollination could improve the pollination success in yam breeding programs. We performed continuous hand pollination within flowering windows of D. rotundata and D. alata for two consecutive years to determine the most appropriate month, week, and hours of the day allowing maximum pollination success. In D. alata crossing block, we observed significant differences among crossing hours for pollination success (p = 0.003); morning hours (8–12 a.m.) being more conducive than afternoons (12–5 p.m.). No significant differences existed between crossing hours in D. rotundata, though the mid-day seemed optimal. For both species, the time interval 11–12 a.m. was more appropriate for crossing while 4–5 p.m. was the poorest. However, in vitro pollen germination tests showed that mid-day pollen collection (12 noon–2 p.m.) had better results than both extremes, though there were strong genotypic effects on outcomes. Pollination success rates differed significantly among months for D. alata (p < 0.001) but not for D. rotundata (p > 0.05). Differences in pollination success existed across weeks within flowering windows of both D. alata (p < 0.001) and D. rotundata (p = 0.004). The seed production efficiency (SPE) had a similar trend as the pollination success rate. No clear pattern existed between the pollination time and the seed setting rate (SSR) or seed viability (SV), though their dynamics varied with weeks and months. This study provided an insight on the dynamics of pollination outcomes under the influence of pollination times and allows detecting months, weeks, and hours of the day when hybridization activities should be focused for better results.
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spelling pubmed-93878362022-08-19 Optimum time for hand pollination in yam (Dioscorea spp.) Mondo, Jean M. Agre, Paterne A. Asiedu, Robert Akoroda, Malachy O. Asfaw, Asrat PLoS One Research Article Hand pollination success rate is low in yam (Dioscorea spp.), due partly to suboptimal weather conditions. Thus, determining the most suitable time for pollination could improve the pollination success in yam breeding programs. We performed continuous hand pollination within flowering windows of D. rotundata and D. alata for two consecutive years to determine the most appropriate month, week, and hours of the day allowing maximum pollination success. In D. alata crossing block, we observed significant differences among crossing hours for pollination success (p = 0.003); morning hours (8–12 a.m.) being more conducive than afternoons (12–5 p.m.). No significant differences existed between crossing hours in D. rotundata, though the mid-day seemed optimal. For both species, the time interval 11–12 a.m. was more appropriate for crossing while 4–5 p.m. was the poorest. However, in vitro pollen germination tests showed that mid-day pollen collection (12 noon–2 p.m.) had better results than both extremes, though there were strong genotypic effects on outcomes. Pollination success rates differed significantly among months for D. alata (p < 0.001) but not for D. rotundata (p > 0.05). Differences in pollination success existed across weeks within flowering windows of both D. alata (p < 0.001) and D. rotundata (p = 0.004). The seed production efficiency (SPE) had a similar trend as the pollination success rate. No clear pattern existed between the pollination time and the seed setting rate (SSR) or seed viability (SV), though their dynamics varied with weeks and months. This study provided an insight on the dynamics of pollination outcomes under the influence of pollination times and allows detecting months, weeks, and hours of the day when hybridization activities should be focused for better results. Public Library of Science 2022-08-18 /pmc/articles/PMC9387836/ /pubmed/35980958 http://dx.doi.org/10.1371/journal.pone.0269670 Text en © 2022 Mondo et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Mondo, Jean M.
Agre, Paterne A.
Asiedu, Robert
Akoroda, Malachy O.
Asfaw, Asrat
Optimum time for hand pollination in yam (Dioscorea spp.)
title Optimum time for hand pollination in yam (Dioscorea spp.)
title_full Optimum time for hand pollination in yam (Dioscorea spp.)
title_fullStr Optimum time for hand pollination in yam (Dioscorea spp.)
title_full_unstemmed Optimum time for hand pollination in yam (Dioscorea spp.)
title_short Optimum time for hand pollination in yam (Dioscorea spp.)
title_sort optimum time for hand pollination in yam (dioscorea spp.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9387836/
https://www.ncbi.nlm.nih.gov/pubmed/35980958
http://dx.doi.org/10.1371/journal.pone.0269670
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