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Effects of hydrogen-rich water and ascorbic acid treatment on spontaneously hypertensive rats

Hydrogen-rich water (HW) has been suggested to possess antioxidant properties of value in treatments of lifestyle diseases and for prevention of latent pathologies. To date, the potential benefits of HW against the deleterious effects of excessive salt intake and hypertension have not been investiga...

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Autores principales: Kawakami, Kohei, Matsuo, Hiroyuki, Yamada, Takaya, Matsumoto, Ken-ichi, Sasaki, Daigoro, Nomura, Masato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9388348/
https://www.ncbi.nlm.nih.gov/pubmed/35264492
http://dx.doi.org/10.1538/expanim.21-0187
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author Kawakami, Kohei
Matsuo, Hiroyuki
Yamada, Takaya
Matsumoto, Ken-ichi
Sasaki, Daigoro
Nomura, Masato
author_facet Kawakami, Kohei
Matsuo, Hiroyuki
Yamada, Takaya
Matsumoto, Ken-ichi
Sasaki, Daigoro
Nomura, Masato
author_sort Kawakami, Kohei
collection PubMed
description Hydrogen-rich water (HW) has been suggested to possess antioxidant properties of value in treatments of lifestyle diseases and for prevention of latent pathologies. To date, the potential benefits of HW against the deleterious effects of excessive salt intake and hypertension have not been investigated. Here, we first examined the effects of HW or HW supplemented with 0.1% ascorbic acid (HWA) on spontaneously hypertensive rats (SHR) that had been fed a normal diet. In comparison to control rats given distilled water (DW), we found that HW did not significantly influence systolic blood pressure (SBP) or diastolic blood pressure (DBP) in SHR; however, the increase in SBP and DBP were inhibited in the HWA group. Next, four groups of SHR were given DW, 0.1% ascorbic acid-added DW (DWA), HW, or HWA in combination with a 4% NaCl-added diet. SHR fed the 4% NaCl-added diet showed increased hypertension; HWA treatment resulted in a significant reduction in blood pressure. The HWA group tended to have lower plasma angiotensin II levels than the DW group. In addition, urinary volumes and urinary sodium levels were significantly lower in the HWA group than the DW group. Urinary isoprostane, an oxidative stress marker, was also significantly lower in the HWA group, suggesting that the inhibitory effect of HWA on blood pressure elevation was caused by a reduction in oxidative stress. These findings suggest a synergistic interaction between HW and ascorbic acid, and also suggest that HWA ingestion has potential for prevention of hypertension.
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spelling pubmed-93883482022-08-24 Effects of hydrogen-rich water and ascorbic acid treatment on spontaneously hypertensive rats Kawakami, Kohei Matsuo, Hiroyuki Yamada, Takaya Matsumoto, Ken-ichi Sasaki, Daigoro Nomura, Masato Exp Anim Original Hydrogen-rich water (HW) has been suggested to possess antioxidant properties of value in treatments of lifestyle diseases and for prevention of latent pathologies. To date, the potential benefits of HW against the deleterious effects of excessive salt intake and hypertension have not been investigated. Here, we first examined the effects of HW or HW supplemented with 0.1% ascorbic acid (HWA) on spontaneously hypertensive rats (SHR) that had been fed a normal diet. In comparison to control rats given distilled water (DW), we found that HW did not significantly influence systolic blood pressure (SBP) or diastolic blood pressure (DBP) in SHR; however, the increase in SBP and DBP were inhibited in the HWA group. Next, four groups of SHR were given DW, 0.1% ascorbic acid-added DW (DWA), HW, or HWA in combination with a 4% NaCl-added diet. SHR fed the 4% NaCl-added diet showed increased hypertension; HWA treatment resulted in a significant reduction in blood pressure. The HWA group tended to have lower plasma angiotensin II levels than the DW group. In addition, urinary volumes and urinary sodium levels were significantly lower in the HWA group than the DW group. Urinary isoprostane, an oxidative stress marker, was also significantly lower in the HWA group, suggesting that the inhibitory effect of HWA on blood pressure elevation was caused by a reduction in oxidative stress. These findings suggest a synergistic interaction between HW and ascorbic acid, and also suggest that HWA ingestion has potential for prevention of hypertension. Japanese Association for Laboratory Animal Science 2022-03-10 2022 /pmc/articles/PMC9388348/ /pubmed/35264492 http://dx.doi.org/10.1538/expanim.21-0187 Text en ©2022 Japanese Association for Laboratory Animal Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original
Kawakami, Kohei
Matsuo, Hiroyuki
Yamada, Takaya
Matsumoto, Ken-ichi
Sasaki, Daigoro
Nomura, Masato
Effects of hydrogen-rich water and ascorbic acid treatment on spontaneously hypertensive rats
title Effects of hydrogen-rich water and ascorbic acid treatment on spontaneously hypertensive rats
title_full Effects of hydrogen-rich water and ascorbic acid treatment on spontaneously hypertensive rats
title_fullStr Effects of hydrogen-rich water and ascorbic acid treatment on spontaneously hypertensive rats
title_full_unstemmed Effects of hydrogen-rich water and ascorbic acid treatment on spontaneously hypertensive rats
title_short Effects of hydrogen-rich water and ascorbic acid treatment on spontaneously hypertensive rats
title_sort effects of hydrogen-rich water and ascorbic acid treatment on spontaneously hypertensive rats
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9388348/
https://www.ncbi.nlm.nih.gov/pubmed/35264492
http://dx.doi.org/10.1538/expanim.21-0187
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