Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation

Stimulated emission depletion (STED) microscopy achieves super-resolution by exciting a diffraction-limited volume and then suppressing fluorescence in its outer parts by depletion. Multiple depletion lasers may introduce misalignment and bleaching. Hence, a single depletion wavelength is preferable...

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Detalles Bibliográficos
Autores principales: Gonzalez Pisfil, Mariano, Nadelson, Iliya, Bergner, Brigitte, Rottmeier, Sonja, Thomae, Andreas W., Dietzel, Steffen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9388687/
https://www.ncbi.nlm.nih.gov/pubmed/35982114
http://dx.doi.org/10.1038/s41598-022-17825-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9388687/
https://www.ncbi.nlm.nih.gov/pubmed/35982114
http://dx.doi.org/10.1038/s41598-022-17825-5

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