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Impact of Lacrimal Gland Extraction on the Contralateral Eye in an Animal Model for Dry Eye Disease
PURPOSE: Although there is still no consensus on the best animal model for dry eye disease research, a model based on lacrimal gland extraction (LGE) model is widely used. In this study, we aimed to investigate the histopathological changes taking place on the contralateral eye after unilateral LGE...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Ophthalmological Society
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9388895/ https://www.ncbi.nlm.nih.gov/pubmed/35766050 http://dx.doi.org/10.3341/kjo.2022.0012 |
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author | Kim, Minha Kim, So Young Jeon, Ji Won Lee, Hyung Keun |
author_facet | Kim, Minha Kim, So Young Jeon, Ji Won Lee, Hyung Keun |
author_sort | Kim, Minha |
collection | PubMed |
description | PURPOSE: Although there is still no consensus on the best animal model for dry eye disease research, a model based on lacrimal gland extraction (LGE) model is widely used. In this study, we aimed to investigate the histopathological changes taking place on the contralateral eye after unilateral LGE to determine whether it is useful as a control. METHODS: Seven-week-old male C57BL/6 mice were divided into naive control, environmental chamber model, and LGE groups. Corneal fluorescein staining was scored to quantify the severity of damage. Morphological changes in the cornea, conjunctiva, and lacrimal gland (LG) were determined by hematoxylin and eosin staining and compared to those on naive control animals. RESULTS: Compared to naive subjects, the unilateral LGE model showed enhanced corneal erosion scores and loss of conjunctival goblet cells, not only on the ipsilateral but also on the contralateral side. These changes in the ocular surface became more pronounced in a time-dependent manner. Furthermore, loss of LG acinar cells and leukocyte infiltration were detected in the contralateral LGs of the LGE model. CONCLUSIONS: Considering the changes observed in the ocular surface and LGs, the contralateral side of the LGE model may not offer proper control conditions for the experimental comparison of the effects of dry eye disease in vivo. There may be regulatory feedback or crosstalk system between both eyes activated in response to LGE. |
format | Online Article Text |
id | pubmed-9388895 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Korean Ophthalmological Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-93888952022-08-22 Impact of Lacrimal Gland Extraction on the Contralateral Eye in an Animal Model for Dry Eye Disease Kim, Minha Kim, So Young Jeon, Ji Won Lee, Hyung Keun Korean J Ophthalmol Original Article PURPOSE: Although there is still no consensus on the best animal model for dry eye disease research, a model based on lacrimal gland extraction (LGE) model is widely used. In this study, we aimed to investigate the histopathological changes taking place on the contralateral eye after unilateral LGE to determine whether it is useful as a control. METHODS: Seven-week-old male C57BL/6 mice were divided into naive control, environmental chamber model, and LGE groups. Corneal fluorescein staining was scored to quantify the severity of damage. Morphological changes in the cornea, conjunctiva, and lacrimal gland (LG) were determined by hematoxylin and eosin staining and compared to those on naive control animals. RESULTS: Compared to naive subjects, the unilateral LGE model showed enhanced corneal erosion scores and loss of conjunctival goblet cells, not only on the ipsilateral but also on the contralateral side. These changes in the ocular surface became more pronounced in a time-dependent manner. Furthermore, loss of LG acinar cells and leukocyte infiltration were detected in the contralateral LGs of the LGE model. CONCLUSIONS: Considering the changes observed in the ocular surface and LGs, the contralateral side of the LGE model may not offer proper control conditions for the experimental comparison of the effects of dry eye disease in vivo. There may be regulatory feedback or crosstalk system between both eyes activated in response to LGE. Korean Ophthalmological Society 2022-08 2022-06-15 /pmc/articles/PMC9388895/ /pubmed/35766050 http://dx.doi.org/10.3341/kjo.2022.0012 Text en © 2022 The Korean Ophthalmological Society https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access journal distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kim, Minha Kim, So Young Jeon, Ji Won Lee, Hyung Keun Impact of Lacrimal Gland Extraction on the Contralateral Eye in an Animal Model for Dry Eye Disease |
title | Impact of Lacrimal Gland Extraction on the Contralateral Eye in an Animal Model for Dry Eye Disease |
title_full | Impact of Lacrimal Gland Extraction on the Contralateral Eye in an Animal Model for Dry Eye Disease |
title_fullStr | Impact of Lacrimal Gland Extraction on the Contralateral Eye in an Animal Model for Dry Eye Disease |
title_full_unstemmed | Impact of Lacrimal Gland Extraction on the Contralateral Eye in an Animal Model for Dry Eye Disease |
title_short | Impact of Lacrimal Gland Extraction on the Contralateral Eye in an Animal Model for Dry Eye Disease |
title_sort | impact of lacrimal gland extraction on the contralateral eye in an animal model for dry eye disease |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9388895/ https://www.ncbi.nlm.nih.gov/pubmed/35766050 http://dx.doi.org/10.3341/kjo.2022.0012 |
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