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Borrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells
Borrelia burgdorferi (Bb), a vector-borne bacterial pathogen and the causative agent of Lyme disease, can spread to distant tissues in the human host by traveling in and through monolayers of endothelial cells (ECs) lining the vasculature. To examine whether Bb alters the physical forces of ECs to p...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9389243/ https://www.ncbi.nlm.nih.gov/pubmed/35992087 http://dx.doi.org/10.1016/j.isci.2022.104793 |
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author | Yuste, Raúl Aparicio Muenkel, Marie Axarlis, Konstantinos Gómez Benito, María J. Reuss, Annalena Blacker, Grace Tal, Michal Caspi Kraiczy, Peter Bastounis, Effie E. |
author_facet | Yuste, Raúl Aparicio Muenkel, Marie Axarlis, Konstantinos Gómez Benito, María J. Reuss, Annalena Blacker, Grace Tal, Michal Caspi Kraiczy, Peter Bastounis, Effie E. |
author_sort | Yuste, Raúl Aparicio |
collection | PubMed |
description | Borrelia burgdorferi (Bb), a vector-borne bacterial pathogen and the causative agent of Lyme disease, can spread to distant tissues in the human host by traveling in and through monolayers of endothelial cells (ECs) lining the vasculature. To examine whether Bb alters the physical forces of ECs to promote its dissemination, we exposed ECs to Bb and observed a sharp and transient increase in EC traction and intercellular forces, followed by a prolonged decrease in EC motility and physical forces. All variables returned to baseline at 24 h after exposure. RNA sequencing analysis revealed an upregulation of innate immune signaling pathways during early but not late Bb exposure. Exposure of ECs to heat-inactivated Bb recapitulated only the early weakening of EC mechanotransduction. The differential responses to live versus heat-inactivated Bb indicate a tight interplay between innate immune signaling and physical forces in host ECs and suggest their active modulation by Bb. |
format | Online Article Text |
id | pubmed-9389243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-93892432022-08-20 Borrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells Yuste, Raúl Aparicio Muenkel, Marie Axarlis, Konstantinos Gómez Benito, María J. Reuss, Annalena Blacker, Grace Tal, Michal Caspi Kraiczy, Peter Bastounis, Effie E. iScience Article Borrelia burgdorferi (Bb), a vector-borne bacterial pathogen and the causative agent of Lyme disease, can spread to distant tissues in the human host by traveling in and through monolayers of endothelial cells (ECs) lining the vasculature. To examine whether Bb alters the physical forces of ECs to promote its dissemination, we exposed ECs to Bb and observed a sharp and transient increase in EC traction and intercellular forces, followed by a prolonged decrease in EC motility and physical forces. All variables returned to baseline at 24 h after exposure. RNA sequencing analysis revealed an upregulation of innate immune signaling pathways during early but not late Bb exposure. Exposure of ECs to heat-inactivated Bb recapitulated only the early weakening of EC mechanotransduction. The differential responses to live versus heat-inactivated Bb indicate a tight interplay between innate immune signaling and physical forces in host ECs and suggest their active modulation by Bb. Elsevier 2022-07-20 /pmc/articles/PMC9389243/ /pubmed/35992087 http://dx.doi.org/10.1016/j.isci.2022.104793 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Yuste, Raúl Aparicio Muenkel, Marie Axarlis, Konstantinos Gómez Benito, María J. Reuss, Annalena Blacker, Grace Tal, Michal Caspi Kraiczy, Peter Bastounis, Effie E. Borrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells |
title | Borrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells |
title_full | Borrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells |
title_fullStr | Borrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells |
title_full_unstemmed | Borrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells |
title_short | Borrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells |
title_sort | borrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9389243/ https://www.ncbi.nlm.nih.gov/pubmed/35992087 http://dx.doi.org/10.1016/j.isci.2022.104793 |
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