Modified protocol for culturing Drosophila S2 R+ cells and adult plasmatocytes to study actin cytoskeleton dynamics

Here, we describe a protocol to culture Drosophila S2R+ cells and to extract plasmatocytes from adult flies. The modified seeding approach detailed here, in combination with coating of coverslips with concanvalin A, enables enhanced adhesion and spreading of cells. We describe the steps for confocal...

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Detalles Bibliográficos
Autores principales: Weaver, Ceileigh M., Makdissi, Stephanie, Di Cara, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9389301/
https://www.ncbi.nlm.nih.gov/pubmed/35990744
http://dx.doi.org/10.1016/j.xpro.2022.101588

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