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Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18)

Microbial-assisted phytoremediation promotes the ecological restoration of high and steep rocky slopes. To determine the structure and function of microbial communities in the soil in response to changes in soil nutrient content, the bacterial communities of rhizospheric soil from three types of pla...

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Autores principales: Liu, Chao, Zhuang, Jiayao, Wang, Jie, Fan, Guohua, Feng, Ming, Zhang, Shutong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9389310/
https://www.ncbi.nlm.nih.gov/pubmed/35992669
http://dx.doi.org/10.3389/fmicb.2022.926037
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author Liu, Chao
Zhuang, Jiayao
Wang, Jie
Fan, Guohua
Feng, Ming
Zhang, Shutong
author_facet Liu, Chao
Zhuang, Jiayao
Wang, Jie
Fan, Guohua
Feng, Ming
Zhang, Shutong
author_sort Liu, Chao
collection PubMed
description Microbial-assisted phytoremediation promotes the ecological restoration of high and steep rocky slopes. To determine the structure and function of microbial communities in the soil in response to changes in soil nutrient content, the bacterial communities of rhizospheric soil from three types of plants, i.e., Robinia pseudoacacia, Pinus massoniana, and Cynodon dactylon, were analyzed using Illumina sequencing technology. High-quality sequences were clustered at the 97% similarity level. The dominant genera were found to be RB41, Gemmatimonas, Sphingomonas, Bradyrhizobium, and Ellin6067. The Tukey HSD (honestly significant difference) test results showed that the abundance of RB41 and Gemmatimonas were significantly different among three types of plants (p < 0.01). The relative abundances of RB41 (13.32%) and Gemmatimonas (3.36%) in rhizospheric soil samples from R. pseudoacacia were significantly higher than that from P. massoniana (0.16 and 0.35%) and C. dactylon (0.40 and 0.82%), respectively. The soil chemical properties analyses suggested that significant differences in rhizospheric soil nutrient content among the three plant types. Especially the available phosphorus, the content of it in the rhizospheric soil of R. pseudoacacia was about 280% (P. massoniana) and 58% (C. dactylon) higher than that of the other two plants, respectively. The soil bacterial communities were further studied using the correlation analysis and the Tax4Fun analysis. A significant and positive correlation was observed between Gemmatimonas and soil nutrient components. Except total nitrogen, the positive correlation between Gemmatimonas and other soil nutrient components was above 0.9. The outcomes of these analyses suggested that Gemmatimonas could be the indicator genus in response to changes in the soil nutrient content. Besides, the genes involved in metabolism were the major contributor to soil nutrients. This study showed that soil nutrients affect the soil bacterial community structure and function. In addition, pot experiments showed that Microbacterium invictum X-18 isolated from the rhizospheric soil of R. pseudoacacia significantly improved soil nutrient content and increased R. pseudoacacia growth. A significant increase in the numbers of nodules of R. pseudoacacia and an increase of 28% in plant height, accompanied by an increase of 94% in available phosphorus was measured in the M. invictum X-18 treatment than the control treatment.
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spelling pubmed-93893102022-08-20 Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18) Liu, Chao Zhuang, Jiayao Wang, Jie Fan, Guohua Feng, Ming Zhang, Shutong Front Microbiol Microbiology Microbial-assisted phytoremediation promotes the ecological restoration of high and steep rocky slopes. To determine the structure and function of microbial communities in the soil in response to changes in soil nutrient content, the bacterial communities of rhizospheric soil from three types of plants, i.e., Robinia pseudoacacia, Pinus massoniana, and Cynodon dactylon, were analyzed using Illumina sequencing technology. High-quality sequences were clustered at the 97% similarity level. The dominant genera were found to be RB41, Gemmatimonas, Sphingomonas, Bradyrhizobium, and Ellin6067. The Tukey HSD (honestly significant difference) test results showed that the abundance of RB41 and Gemmatimonas were significantly different among three types of plants (p < 0.01). The relative abundances of RB41 (13.32%) and Gemmatimonas (3.36%) in rhizospheric soil samples from R. pseudoacacia were significantly higher than that from P. massoniana (0.16 and 0.35%) and C. dactylon (0.40 and 0.82%), respectively. The soil chemical properties analyses suggested that significant differences in rhizospheric soil nutrient content among the three plant types. Especially the available phosphorus, the content of it in the rhizospheric soil of R. pseudoacacia was about 280% (P. massoniana) and 58% (C. dactylon) higher than that of the other two plants, respectively. The soil bacterial communities were further studied using the correlation analysis and the Tax4Fun analysis. A significant and positive correlation was observed between Gemmatimonas and soil nutrient components. Except total nitrogen, the positive correlation between Gemmatimonas and other soil nutrient components was above 0.9. The outcomes of these analyses suggested that Gemmatimonas could be the indicator genus in response to changes in the soil nutrient content. Besides, the genes involved in metabolism were the major contributor to soil nutrients. This study showed that soil nutrients affect the soil bacterial community structure and function. In addition, pot experiments showed that Microbacterium invictum X-18 isolated from the rhizospheric soil of R. pseudoacacia significantly improved soil nutrient content and increased R. pseudoacacia growth. A significant increase in the numbers of nodules of R. pseudoacacia and an increase of 28% in plant height, accompanied by an increase of 94% in available phosphorus was measured in the M. invictum X-18 treatment than the control treatment. Frontiers Media S.A. 2022-08-05 /pmc/articles/PMC9389310/ /pubmed/35992669 http://dx.doi.org/10.3389/fmicb.2022.926037 Text en Copyright © 2022 Liu, Zhuang, Wang, Fan, Feng and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Chao
Zhuang, Jiayao
Wang, Jie
Fan, Guohua
Feng, Ming
Zhang, Shutong
Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18)
title Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18)
title_full Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18)
title_fullStr Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18)
title_full_unstemmed Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18)
title_short Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18)
title_sort soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of microbacterium invictum (strain x-18)
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9389310/
https://www.ncbi.nlm.nih.gov/pubmed/35992669
http://dx.doi.org/10.3389/fmicb.2022.926037
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