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Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice

Renal ischemia–reperfusion injury (IRI) is a common prerequisite of acute renal injury (AKI) that involves the entire system and induces critical illness. The C domain of insulin-like growth factor-1 (IGF-1C) plays an important role in promoting angiogenesis and enhancing the inflammatory response....

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Autores principales: Xu, Meng, Zhao, Mingyue, Zheng, Donghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9389927/
https://www.ncbi.nlm.nih.gov/pubmed/35969012
http://dx.doi.org/10.1080/0886022X.2022.2098773
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author Xu, Meng
Zhao, Mingyue
Zheng, Donghui
author_facet Xu, Meng
Zhao, Mingyue
Zheng, Donghui
author_sort Xu, Meng
collection PubMed
description Renal ischemia–reperfusion injury (IRI) is a common prerequisite of acute renal injury (AKI) that involves the entire system and induces critical illness. The C domain of insulin-like growth factor-1 (IGF-1C) plays an important role in promoting angiogenesis and enhancing the inflammatory response. However, given the shortcomings of its short half-life and poor stability, the application of IGF-1C is restricted. In the present study, IGF-1C nanoparticles (NP-IGF-1C) were constructed by combining 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide (polye thyleneglycol)](DSPE-PEG-MAL) and IGF-1C through a Michael addition reaction to evaluate the effects of NP-IGF-1C on preventing IRI. In vitro studies have shown that NP-IGF-1C is not cytotoxic and protects cells from oxidative damage. The renal enrichment and biocompatibility of NP-IGF-1C were determined in vivo by connecting fluorescent molecules to NP-IGF-1C for in vivo imaging and pathological staining of important organs. After IRI, renal function decreased, and inflammatory cell infiltration, oxidative stress and apoptosis increased. As expected, NP-IGF-1C reversed these changes, indicating that NP-IGF-1C played a protective role in the process of IRI, which may be mediated by its antioxidant, anti-inflammatory and antiapoptotic activities.
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spelling pubmed-93899272022-08-20 Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice Xu, Meng Zhao, Mingyue Zheng, Donghui Ren Fail Laboratory Study Renal ischemia–reperfusion injury (IRI) is a common prerequisite of acute renal injury (AKI) that involves the entire system and induces critical illness. The C domain of insulin-like growth factor-1 (IGF-1C) plays an important role in promoting angiogenesis and enhancing the inflammatory response. However, given the shortcomings of its short half-life and poor stability, the application of IGF-1C is restricted. In the present study, IGF-1C nanoparticles (NP-IGF-1C) were constructed by combining 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide (polye thyleneglycol)](DSPE-PEG-MAL) and IGF-1C through a Michael addition reaction to evaluate the effects of NP-IGF-1C on preventing IRI. In vitro studies have shown that NP-IGF-1C is not cytotoxic and protects cells from oxidative damage. The renal enrichment and biocompatibility of NP-IGF-1C were determined in vivo by connecting fluorescent molecules to NP-IGF-1C for in vivo imaging and pathological staining of important organs. After IRI, renal function decreased, and inflammatory cell infiltration, oxidative stress and apoptosis increased. As expected, NP-IGF-1C reversed these changes, indicating that NP-IGF-1C played a protective role in the process of IRI, which may be mediated by its antioxidant, anti-inflammatory and antiapoptotic activities. Taylor & Francis 2022-08-15 /pmc/articles/PMC9389927/ /pubmed/35969012 http://dx.doi.org/10.1080/0886022X.2022.2098773 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Laboratory Study
Xu, Meng
Zhao, Mingyue
Zheng, Donghui
Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice
title Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice
title_full Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice
title_fullStr Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice
title_full_unstemmed Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice
title_short Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice
title_sort effect of igf-1c domain-modified nanoparticles on renal ischemia–reperfusion injury in mice
topic Laboratory Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9389927/
https://www.ncbi.nlm.nih.gov/pubmed/35969012
http://dx.doi.org/10.1080/0886022X.2022.2098773
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