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Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice
Renal ischemia–reperfusion injury (IRI) is a common prerequisite of acute renal injury (AKI) that involves the entire system and induces critical illness. The C domain of insulin-like growth factor-1 (IGF-1C) plays an important role in promoting angiogenesis and enhancing the inflammatory response....
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9389927/ https://www.ncbi.nlm.nih.gov/pubmed/35969012 http://dx.doi.org/10.1080/0886022X.2022.2098773 |
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author | Xu, Meng Zhao, Mingyue Zheng, Donghui |
author_facet | Xu, Meng Zhao, Mingyue Zheng, Donghui |
author_sort | Xu, Meng |
collection | PubMed |
description | Renal ischemia–reperfusion injury (IRI) is a common prerequisite of acute renal injury (AKI) that involves the entire system and induces critical illness. The C domain of insulin-like growth factor-1 (IGF-1C) plays an important role in promoting angiogenesis and enhancing the inflammatory response. However, given the shortcomings of its short half-life and poor stability, the application of IGF-1C is restricted. In the present study, IGF-1C nanoparticles (NP-IGF-1C) were constructed by combining 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide (polye thyleneglycol)](DSPE-PEG-MAL) and IGF-1C through a Michael addition reaction to evaluate the effects of NP-IGF-1C on preventing IRI. In vitro studies have shown that NP-IGF-1C is not cytotoxic and protects cells from oxidative damage. The renal enrichment and biocompatibility of NP-IGF-1C were determined in vivo by connecting fluorescent molecules to NP-IGF-1C for in vivo imaging and pathological staining of important organs. After IRI, renal function decreased, and inflammatory cell infiltration, oxidative stress and apoptosis increased. As expected, NP-IGF-1C reversed these changes, indicating that NP-IGF-1C played a protective role in the process of IRI, which may be mediated by its antioxidant, anti-inflammatory and antiapoptotic activities. |
format | Online Article Text |
id | pubmed-9389927 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-93899272022-08-20 Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice Xu, Meng Zhao, Mingyue Zheng, Donghui Ren Fail Laboratory Study Renal ischemia–reperfusion injury (IRI) is a common prerequisite of acute renal injury (AKI) that involves the entire system and induces critical illness. The C domain of insulin-like growth factor-1 (IGF-1C) plays an important role in promoting angiogenesis and enhancing the inflammatory response. However, given the shortcomings of its short half-life and poor stability, the application of IGF-1C is restricted. In the present study, IGF-1C nanoparticles (NP-IGF-1C) were constructed by combining 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide (polye thyleneglycol)](DSPE-PEG-MAL) and IGF-1C through a Michael addition reaction to evaluate the effects of NP-IGF-1C on preventing IRI. In vitro studies have shown that NP-IGF-1C is not cytotoxic and protects cells from oxidative damage. The renal enrichment and biocompatibility of NP-IGF-1C were determined in vivo by connecting fluorescent molecules to NP-IGF-1C for in vivo imaging and pathological staining of important organs. After IRI, renal function decreased, and inflammatory cell infiltration, oxidative stress and apoptosis increased. As expected, NP-IGF-1C reversed these changes, indicating that NP-IGF-1C played a protective role in the process of IRI, which may be mediated by its antioxidant, anti-inflammatory and antiapoptotic activities. Taylor & Francis 2022-08-15 /pmc/articles/PMC9389927/ /pubmed/35969012 http://dx.doi.org/10.1080/0886022X.2022.2098773 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Laboratory Study Xu, Meng Zhao, Mingyue Zheng, Donghui Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice |
title | Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice |
title_full | Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice |
title_fullStr | Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice |
title_full_unstemmed | Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice |
title_short | Effect of IGF-1C domain-modified nanoparticles on renal ischemia–reperfusion injury in mice |
title_sort | effect of igf-1c domain-modified nanoparticles on renal ischemia–reperfusion injury in mice |
topic | Laboratory Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9389927/ https://www.ncbi.nlm.nih.gov/pubmed/35969012 http://dx.doi.org/10.1080/0886022X.2022.2098773 |
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