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Effect of electroacupuncture on the intestinal microflora in rats with stress urinary incontinence

OBJECTIVE: To examine the effect of electroacupuncture on the urodynamics and gut microbiota of rats with stress urinary incontinence (SUI). MATERIALS AND METHODS: Thirty 2-month-old female Sprague–Dawley (SD) rats were randomly assigned to 4 groups: normal (N), model (M), nonacupoint electric acupu...

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Autores principales: Li, Chaonan, Qu, Zhiyu, Liu, Jiandang, Ruan, Shuoquan, Chen, Bingli, Ran, Jinchuan, Shu, Wen, Chen, Yuelai, Hou, Wenguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9390059/
https://www.ncbi.nlm.nih.gov/pubmed/35992152
http://dx.doi.org/10.3389/fendo.2022.860100
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author Li, Chaonan
Qu, Zhiyu
Liu, Jiandang
Ruan, Shuoquan
Chen, Bingli
Ran, Jinchuan
Shu, Wen
Chen, Yuelai
Hou, Wenguang
author_facet Li, Chaonan
Qu, Zhiyu
Liu, Jiandang
Ruan, Shuoquan
Chen, Bingli
Ran, Jinchuan
Shu, Wen
Chen, Yuelai
Hou, Wenguang
author_sort Li, Chaonan
collection PubMed
description OBJECTIVE: To examine the effect of electroacupuncture on the urodynamics and gut microbiota of rats with stress urinary incontinence (SUI). MATERIALS AND METHODS: Thirty 2-month-old female Sprague–Dawley (SD) rats were randomly assigned to 4 groups: normal (N), model (M), nonacupoint electric acupuncture control (NAAC), and electroacupuncture (EA). An SUI rat model was established through vaginal balloon dilatation and bilateral oophorectomy. After various treatments, urodynamic tests were performed, and feces were collected. 16S rRNA sequencing analysis was used to investigate SUI-related changes in the intestinal flora. RESULTS: After treatment, compared with those of the M group, the leak point pressure and maximum bladder capacity of the electroacupuncture groups increased (P<0.05). The species community compositions of the N and M groups differed at the genus level, and there were 15 differentially abundant bacterial genera (P<0.05). The Blautia proportion was increased by electroacupuncture treatment (P<0.05) and was significantly positively correlated with the electroacupuncture treatment of SUI (according to Spearman correlation analysis). CONCLUSION: Electroacupuncture treatment can improve signs of urine leakage in rats with SUI rats by increasing the leak point pressure and maximum bladder capacity. The enrichment of Blautia by electroacupuncture treatment enrichment may be related to SUI sign improvement.
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spelling pubmed-93900592022-08-20 Effect of electroacupuncture on the intestinal microflora in rats with stress urinary incontinence Li, Chaonan Qu, Zhiyu Liu, Jiandang Ruan, Shuoquan Chen, Bingli Ran, Jinchuan Shu, Wen Chen, Yuelai Hou, Wenguang Front Endocrinol (Lausanne) Endocrinology OBJECTIVE: To examine the effect of electroacupuncture on the urodynamics and gut microbiota of rats with stress urinary incontinence (SUI). MATERIALS AND METHODS: Thirty 2-month-old female Sprague–Dawley (SD) rats were randomly assigned to 4 groups: normal (N), model (M), nonacupoint electric acupuncture control (NAAC), and electroacupuncture (EA). An SUI rat model was established through vaginal balloon dilatation and bilateral oophorectomy. After various treatments, urodynamic tests were performed, and feces were collected. 16S rRNA sequencing analysis was used to investigate SUI-related changes in the intestinal flora. RESULTS: After treatment, compared with those of the M group, the leak point pressure and maximum bladder capacity of the electroacupuncture groups increased (P<0.05). The species community compositions of the N and M groups differed at the genus level, and there were 15 differentially abundant bacterial genera (P<0.05). The Blautia proportion was increased by electroacupuncture treatment (P<0.05) and was significantly positively correlated with the electroacupuncture treatment of SUI (according to Spearman correlation analysis). CONCLUSION: Electroacupuncture treatment can improve signs of urine leakage in rats with SUI rats by increasing the leak point pressure and maximum bladder capacity. The enrichment of Blautia by electroacupuncture treatment enrichment may be related to SUI sign improvement. Frontiers Media S.A. 2022-07-29 /pmc/articles/PMC9390059/ /pubmed/35992152 http://dx.doi.org/10.3389/fendo.2022.860100 Text en Copyright © 2022 Li, Qu, Liu, Ruan, Chen, Ran, Shu, Chen and Hou https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Li, Chaonan
Qu, Zhiyu
Liu, Jiandang
Ruan, Shuoquan
Chen, Bingli
Ran, Jinchuan
Shu, Wen
Chen, Yuelai
Hou, Wenguang
Effect of electroacupuncture on the intestinal microflora in rats with stress urinary incontinence
title Effect of electroacupuncture on the intestinal microflora in rats with stress urinary incontinence
title_full Effect of electroacupuncture on the intestinal microflora in rats with stress urinary incontinence
title_fullStr Effect of electroacupuncture on the intestinal microflora in rats with stress urinary incontinence
title_full_unstemmed Effect of electroacupuncture on the intestinal microflora in rats with stress urinary incontinence
title_short Effect of electroacupuncture on the intestinal microflora in rats with stress urinary incontinence
title_sort effect of electroacupuncture on the intestinal microflora in rats with stress urinary incontinence
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9390059/
https://www.ncbi.nlm.nih.gov/pubmed/35992152
http://dx.doi.org/10.3389/fendo.2022.860100
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