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Freshwater macrophytes harbor viruses representing all five major phyla of the RNA viral kingdom Orthornavirae
Research on aquatic plant viruses is lagging behind that of their terrestrial counterparts. To address this knowledge gap, here we identified viruses associated with freshwater macrophytes, a taxonomically diverse group of aquatic phototrophs that are visible with the naked eye. We surveyed pooled m...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9390326/ https://www.ncbi.nlm.nih.gov/pubmed/35990902 http://dx.doi.org/10.7717/peerj.13875 |
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author | Rosario, Karyna Van Bogaert, Noémi López-Figueroa, Natalia B. Paliogiannis, Haris Kerr, Mason Breitbart, Mya |
author_facet | Rosario, Karyna Van Bogaert, Noémi López-Figueroa, Natalia B. Paliogiannis, Haris Kerr, Mason Breitbart, Mya |
author_sort | Rosario, Karyna |
collection | PubMed |
description | Research on aquatic plant viruses is lagging behind that of their terrestrial counterparts. To address this knowledge gap, here we identified viruses associated with freshwater macrophytes, a taxonomically diverse group of aquatic phototrophs that are visible with the naked eye. We surveyed pooled macrophyte samples collected at four spring sites in Florida, USA through next generation sequencing of RNA extracted from purified viral particles. Sequencing efforts resulted in the detection of 156 freshwater macrophyte associated (FMA) viral contigs, 37 of which approximate complete genomes or segments. FMA viral contigs represent putative members from all five major phyla of the RNA viral kingdom Orthornavirae. Similar to viral types found in land plants, viral sequences identified in macrophytes were dominated by positive-sense RNA viruses. Over half of the FMA viral contigs were most similar to viruses reported from diverse hosts in aquatic environments, including phototrophs, invertebrates, and fungi. The detection of FMA viruses from orders dominated by plant viruses, namely Patatavirales and Tymovirales, indicate that members of these orders may thrive in aquatic hosts. PCR assays confirmed the presence of putative FMA plant viruses in asymptomatic vascular plants, indicating that viruses with persistent lifestyles are widespread in macrophytes. The detection of potato virus Y and oat blue dwarf virus in submerged macrophytes suggests that terrestrial plant viruses infect underwater plants and highlights a potential terrestrial-freshwater plant virus continuum. Defining the virome of unexplored macrophytes will improve our understanding of virus evolution in terrestrial and aquatic primary producers and reveal the potential ecological impacts of viral infection in macrophytes. |
format | Online Article Text |
id | pubmed-9390326 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93903262022-08-20 Freshwater macrophytes harbor viruses representing all five major phyla of the RNA viral kingdom Orthornavirae Rosario, Karyna Van Bogaert, Noémi López-Figueroa, Natalia B. Paliogiannis, Haris Kerr, Mason Breitbart, Mya PeerJ Genomics Research on aquatic plant viruses is lagging behind that of their terrestrial counterparts. To address this knowledge gap, here we identified viruses associated with freshwater macrophytes, a taxonomically diverse group of aquatic phototrophs that are visible with the naked eye. We surveyed pooled macrophyte samples collected at four spring sites in Florida, USA through next generation sequencing of RNA extracted from purified viral particles. Sequencing efforts resulted in the detection of 156 freshwater macrophyte associated (FMA) viral contigs, 37 of which approximate complete genomes or segments. FMA viral contigs represent putative members from all five major phyla of the RNA viral kingdom Orthornavirae. Similar to viral types found in land plants, viral sequences identified in macrophytes were dominated by positive-sense RNA viruses. Over half of the FMA viral contigs were most similar to viruses reported from diverse hosts in aquatic environments, including phototrophs, invertebrates, and fungi. The detection of FMA viruses from orders dominated by plant viruses, namely Patatavirales and Tymovirales, indicate that members of these orders may thrive in aquatic hosts. PCR assays confirmed the presence of putative FMA plant viruses in asymptomatic vascular plants, indicating that viruses with persistent lifestyles are widespread in macrophytes. The detection of potato virus Y and oat blue dwarf virus in submerged macrophytes suggests that terrestrial plant viruses infect underwater plants and highlights a potential terrestrial-freshwater plant virus continuum. Defining the virome of unexplored macrophytes will improve our understanding of virus evolution in terrestrial and aquatic primary producers and reveal the potential ecological impacts of viral infection in macrophytes. PeerJ Inc. 2022-08-16 /pmc/articles/PMC9390326/ /pubmed/35990902 http://dx.doi.org/10.7717/peerj.13875 Text en © 2022 Rosario et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Genomics Rosario, Karyna Van Bogaert, Noémi López-Figueroa, Natalia B. Paliogiannis, Haris Kerr, Mason Breitbart, Mya Freshwater macrophytes harbor viruses representing all five major phyla of the RNA viral kingdom Orthornavirae |
title | Freshwater macrophytes harbor viruses representing all five major phyla of the RNA viral kingdom Orthornavirae |
title_full | Freshwater macrophytes harbor viruses representing all five major phyla of the RNA viral kingdom Orthornavirae |
title_fullStr | Freshwater macrophytes harbor viruses representing all five major phyla of the RNA viral kingdom Orthornavirae |
title_full_unstemmed | Freshwater macrophytes harbor viruses representing all five major phyla of the RNA viral kingdom Orthornavirae |
title_short | Freshwater macrophytes harbor viruses representing all five major phyla of the RNA viral kingdom Orthornavirae |
title_sort | freshwater macrophytes harbor viruses representing all five major phyla of the rna viral kingdom orthornavirae |
topic | Genomics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9390326/ https://www.ncbi.nlm.nih.gov/pubmed/35990902 http://dx.doi.org/10.7717/peerj.13875 |
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