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Evaluation of small-diameter silk vascular grafts implanted in dogs

OBJECTIVES: Vascular replacement is one treatment for cardiovascular disease. However, in blood vessels with a diameter less than 6 mm, the existing artificial vascular grafts may be occluded by thrombus formation or intimal hyperplasia. Thus, new artificial vascular grafts need to be developed. We...

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Autores principales: Tanaka, Takashi, Tanaka, Ryo, Ogawa, Yoko, Takagi, Yoshihide, Sata, Masataka, Asakura, Tetsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9390453/
https://www.ncbi.nlm.nih.gov/pubmed/36003556
http://dx.doi.org/10.1016/j.xjon.2021.02.008
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author Tanaka, Takashi
Tanaka, Ryo
Ogawa, Yoko
Takagi, Yoshihide
Sata, Masataka
Asakura, Tetsuo
author_facet Tanaka, Takashi
Tanaka, Ryo
Ogawa, Yoko
Takagi, Yoshihide
Sata, Masataka
Asakura, Tetsuo
author_sort Tanaka, Takashi
collection PubMed
description OBJECTIVES: Vascular replacement is one treatment for cardiovascular disease. However, in blood vessels with a diameter less than 6 mm, the existing artificial vascular grafts may be occluded by thrombus formation or intimal hyperplasia. Thus, new artificial vascular grafts need to be developed. We have developed a small-diameter artificial vascular graft made of silk fibroin. The implantation of such graft has been evaluated mainly in rats. However, only a few reports describe long-term implantation in large animal models. Therefore, modified silk fibroin artificial vascular grafts were implanted in the femoral arteries of dogs, and their patency and remodeling ability were investigated. METHODS: Six beagles weighing 10 to 12 kg were used for the in vivo study. Grafts (4 cm length × 3.5 mm inner diameter) were implanted in the femoral artery of 6 dogs. The patency of the graft was monitored using vascular ultrasound apparatus. At 3 months', 5 months', and 1-year postimplantation, the graft was retrieved and conducted histopathologic examination. RESULTS: No side effects, such as ischemia, paralysis, and edema of the hind legs, were observed postimplantation. Five of the 6 grafts exhibited a high patency rate, and the lumen was covered with vascular endothelial cells in the central part of the graft 3 months’ postimplantation. CONCLUSIONS: Based on these results, artificial silk fibroin vascular grafts implanted in the femoral arteries of dogs exhibit high patency and remodeling ability. Silk fibroin grafts may be clinically applicable as an artificial vascular graft in small-diameter <6 mm.
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spelling pubmed-93904532022-08-23 Evaluation of small-diameter silk vascular grafts implanted in dogs Tanaka, Takashi Tanaka, Ryo Ogawa, Yoko Takagi, Yoshihide Sata, Masataka Asakura, Tetsuo JTCVS Open Adult: Coronary: Basic Science OBJECTIVES: Vascular replacement is one treatment for cardiovascular disease. However, in blood vessels with a diameter less than 6 mm, the existing artificial vascular grafts may be occluded by thrombus formation or intimal hyperplasia. Thus, new artificial vascular grafts need to be developed. We have developed a small-diameter artificial vascular graft made of silk fibroin. The implantation of such graft has been evaluated mainly in rats. However, only a few reports describe long-term implantation in large animal models. Therefore, modified silk fibroin artificial vascular grafts were implanted in the femoral arteries of dogs, and their patency and remodeling ability were investigated. METHODS: Six beagles weighing 10 to 12 kg were used for the in vivo study. Grafts (4 cm length × 3.5 mm inner diameter) were implanted in the femoral artery of 6 dogs. The patency of the graft was monitored using vascular ultrasound apparatus. At 3 months', 5 months', and 1-year postimplantation, the graft was retrieved and conducted histopathologic examination. RESULTS: No side effects, such as ischemia, paralysis, and edema of the hind legs, were observed postimplantation. Five of the 6 grafts exhibited a high patency rate, and the lumen was covered with vascular endothelial cells in the central part of the graft 3 months’ postimplantation. CONCLUSIONS: Based on these results, artificial silk fibroin vascular grafts implanted in the femoral arteries of dogs exhibit high patency and remodeling ability. Silk fibroin grafts may be clinically applicable as an artificial vascular graft in small-diameter <6 mm. Elsevier 2021-03-04 /pmc/articles/PMC9390453/ /pubmed/36003556 http://dx.doi.org/10.1016/j.xjon.2021.02.008 Text en © 2021 The Authors. Published by Elsevier Inc. on behalf of The American Association for Thoracic Surgery. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Adult: Coronary: Basic Science
Tanaka, Takashi
Tanaka, Ryo
Ogawa, Yoko
Takagi, Yoshihide
Sata, Masataka
Asakura, Tetsuo
Evaluation of small-diameter silk vascular grafts implanted in dogs
title Evaluation of small-diameter silk vascular grafts implanted in dogs
title_full Evaluation of small-diameter silk vascular grafts implanted in dogs
title_fullStr Evaluation of small-diameter silk vascular grafts implanted in dogs
title_full_unstemmed Evaluation of small-diameter silk vascular grafts implanted in dogs
title_short Evaluation of small-diameter silk vascular grafts implanted in dogs
title_sort evaluation of small-diameter silk vascular grafts implanted in dogs
topic Adult: Coronary: Basic Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9390453/
https://www.ncbi.nlm.nih.gov/pubmed/36003556
http://dx.doi.org/10.1016/j.xjon.2021.02.008
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