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Influence of the environment on ragweed pollen and their sensitizing capacity in a mouse model of allergic lung inflammation

Common ragweed (Ambrosia artemisiifolia) is an invasive plant with allergenic pollen. Due to environmental changes, ragweed pollen (RWP) airborne concentrations are predicted to quadruple in Europe by 2050 and more than double allergic sensitization of Europeans by 2060. We developed an experimental...

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Autores principales: Liu, Shu-Hua, Kazemi, Sahar, Karrer, Gerhard, Bellaire, Anke, Weckwerth, Wolfram, Damkjaer, Jakob, Hoffmann, Oskar, Epstein, Michelle M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9390857/
https://www.ncbi.nlm.nih.gov/pubmed/35991309
http://dx.doi.org/10.3389/falgy.2022.854038
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author Liu, Shu-Hua
Kazemi, Sahar
Karrer, Gerhard
Bellaire, Anke
Weckwerth, Wolfram
Damkjaer, Jakob
Hoffmann, Oskar
Epstein, Michelle M.
author_facet Liu, Shu-Hua
Kazemi, Sahar
Karrer, Gerhard
Bellaire, Anke
Weckwerth, Wolfram
Damkjaer, Jakob
Hoffmann, Oskar
Epstein, Michelle M.
author_sort Liu, Shu-Hua
collection PubMed
description Common ragweed (Ambrosia artemisiifolia) is an invasive plant with allergenic pollen. Due to environmental changes, ragweed pollen (RWP) airborne concentrations are predicted to quadruple in Europe by 2050 and more than double allergic sensitization of Europeans by 2060. We developed an experimental RWP model of allergy in BALB/c mice to evaluate how the number of RWP and how RWP collected from different geographical environments influence disease. We administered RWP six times over 3 weeks intranasally to the mice and then evaluated disease parameters 72 h later or allowed the mice to recover for at least 90 days before rechallenging them with RWP to elicit a disease relapse. Doses over 300 pollen grains induced lung eosinophilia. Higher doses of 3,000 and 30,000 pollen grains increased both eosinophils and neutrophils and induced disease relapses. RWP harvested from diverse geographical regions induced a spectrum of allergic lung disease from mild inflammation to moderate eosinophilic and severe mixed eosinophilic-neutrophilic lung infiltrates. After a recovery period, mice rechallenged with pollen developed a robust disease relapse. We found no correlation between Amb a 1 content, the major immunodominant allergen, endotoxin content, or RWP structure with disease severity. These results demonstrate that there is an environmental impact on RWP with clinical consequences that may underlie the increasing sensitization rates and the severity of pollen-induced disease exacerbation in patients. The multitude of diverse environmental factors governing distinctive patterns of disease induced by RWP remains unclear. Further studies are necessary to elucidate how the environment influences the complex interaction between RWP and human health.
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spelling pubmed-93908572022-08-20 Influence of the environment on ragweed pollen and their sensitizing capacity in a mouse model of allergic lung inflammation Liu, Shu-Hua Kazemi, Sahar Karrer, Gerhard Bellaire, Anke Weckwerth, Wolfram Damkjaer, Jakob Hoffmann, Oskar Epstein, Michelle M. Front Allergy Allergy Common ragweed (Ambrosia artemisiifolia) is an invasive plant with allergenic pollen. Due to environmental changes, ragweed pollen (RWP) airborne concentrations are predicted to quadruple in Europe by 2050 and more than double allergic sensitization of Europeans by 2060. We developed an experimental RWP model of allergy in BALB/c mice to evaluate how the number of RWP and how RWP collected from different geographical environments influence disease. We administered RWP six times over 3 weeks intranasally to the mice and then evaluated disease parameters 72 h later or allowed the mice to recover for at least 90 days before rechallenging them with RWP to elicit a disease relapse. Doses over 300 pollen grains induced lung eosinophilia. Higher doses of 3,000 and 30,000 pollen grains increased both eosinophils and neutrophils and induced disease relapses. RWP harvested from diverse geographical regions induced a spectrum of allergic lung disease from mild inflammation to moderate eosinophilic and severe mixed eosinophilic-neutrophilic lung infiltrates. After a recovery period, mice rechallenged with pollen developed a robust disease relapse. We found no correlation between Amb a 1 content, the major immunodominant allergen, endotoxin content, or RWP structure with disease severity. These results demonstrate that there is an environmental impact on RWP with clinical consequences that may underlie the increasing sensitization rates and the severity of pollen-induced disease exacerbation in patients. The multitude of diverse environmental factors governing distinctive patterns of disease induced by RWP remains unclear. Further studies are necessary to elucidate how the environment influences the complex interaction between RWP and human health. Frontiers Media S.A. 2022-08-05 /pmc/articles/PMC9390857/ /pubmed/35991309 http://dx.doi.org/10.3389/falgy.2022.854038 Text en Copyright © 2022 Liu, Kazemi, Karrer, Bellaire, Weckwerth, Damkjaer, Hoffmann and Epstein. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Allergy
Liu, Shu-Hua
Kazemi, Sahar
Karrer, Gerhard
Bellaire, Anke
Weckwerth, Wolfram
Damkjaer, Jakob
Hoffmann, Oskar
Epstein, Michelle M.
Influence of the environment on ragweed pollen and their sensitizing capacity in a mouse model of allergic lung inflammation
title Influence of the environment on ragweed pollen and their sensitizing capacity in a mouse model of allergic lung inflammation
title_full Influence of the environment on ragweed pollen and their sensitizing capacity in a mouse model of allergic lung inflammation
title_fullStr Influence of the environment on ragweed pollen and their sensitizing capacity in a mouse model of allergic lung inflammation
title_full_unstemmed Influence of the environment on ragweed pollen and their sensitizing capacity in a mouse model of allergic lung inflammation
title_short Influence of the environment on ragweed pollen and their sensitizing capacity in a mouse model of allergic lung inflammation
title_sort influence of the environment on ragweed pollen and their sensitizing capacity in a mouse model of allergic lung inflammation
topic Allergy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9390857/
https://www.ncbi.nlm.nih.gov/pubmed/35991309
http://dx.doi.org/10.3389/falgy.2022.854038
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