Aggregation of cryopreserved mid-hindgut endoderm for more reliable and reproducible hPSC-derived small intestinal organoid generation

A major technical limitation hindering the widespread adoption of human pluripotent stem cell (hPSC)-derived gastrointestinal (GI) organoid technologies is the need for de novo hPSC differentiation and dependence on spontaneous morphogenesis to produce detached spheroids. Here, we report a method fo...

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Detalles Bibliográficos
Autores principales: Pitstick, Amy L., Poling, Holly M., Sundaram, Nambirajan, Lewis, Phillip L., Kechele, Daniel O., Sanchez, J. Guillermo, Scott, Melissa A., Broda, Taylor R., Helmrath, Michael A., Wells, James M., Mayhew, Christopher N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9391520/
https://www.ncbi.nlm.nih.gov/pubmed/35905739
http://dx.doi.org/10.1016/j.stemcr.2022.06.011

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