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Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions
The yeast phase of 22 Histoplasma capsulatum clinical isolates from Mexico, Argentina, Colombia, and Guatemala and three reference strains, one from Panama and two from the United States of America (USA), were screened for thermosensitivity characteristics using different analyses. Growth curves at...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9392019/ https://www.ncbi.nlm.nih.gov/pubmed/31987782 http://dx.doi.org/10.1016/j.bjid.2019.12.007 |
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author | Sahaza, Jorge H. Rodríguez-Arellanez, Gabriela Canteros, Cristina E. Reyes-Montes, María del Rocío Taylor, Maria Lucia |
author_facet | Sahaza, Jorge H. Rodríguez-Arellanez, Gabriela Canteros, Cristina E. Reyes-Montes, María del Rocío Taylor, Maria Lucia |
author_sort | Sahaza, Jorge H. |
collection | PubMed |
description | The yeast phase of 22 Histoplasma capsulatum clinical isolates from Mexico, Argentina, Colombia, and Guatemala and three reference strains, one from Panama and two from the United States of America (USA), were screened for thermosensitivity characteristics using different analyses. Growth curves at 0, 3, 6, 12, 24, and 30 h of incubation at 37 and 40 °C, the growth inhibition percentage at 40 °C, and the doubling time at 37 and 40 °C were determined for all yeasts studied. Most of the isolates examined exhibited thermotolerant phenotypes at 40 °C, whereas a thermosensitive phenotype at 40 °C was only detected in the Downs reference strain from the USA. Growth inhibition values lower than 33.8% supported the predominance of the thermotolerant phenotype at 40 °C. The doubling time means found for the different isolates were 5.14 h ± 1.47 h at 37 °C and 5.55 h ± 1.87 h at 40 °C. This is the first report to underscore the predominance of thermotolerant and delayed doubling time phenotypes in H. capsulatum clinical isolates from different regions of Latin America. |
format | Online Article Text |
id | pubmed-9392019 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-93920192022-08-23 Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions Sahaza, Jorge H. Rodríguez-Arellanez, Gabriela Canteros, Cristina E. Reyes-Montes, María del Rocío Taylor, Maria Lucia Braz J Infect Dis Original Article The yeast phase of 22 Histoplasma capsulatum clinical isolates from Mexico, Argentina, Colombia, and Guatemala and three reference strains, one from Panama and two from the United States of America (USA), were screened for thermosensitivity characteristics using different analyses. Growth curves at 0, 3, 6, 12, 24, and 30 h of incubation at 37 and 40 °C, the growth inhibition percentage at 40 °C, and the doubling time at 37 and 40 °C were determined for all yeasts studied. Most of the isolates examined exhibited thermotolerant phenotypes at 40 °C, whereas a thermosensitive phenotype at 40 °C was only detected in the Downs reference strain from the USA. Growth inhibition values lower than 33.8% supported the predominance of the thermotolerant phenotype at 40 °C. The doubling time means found for the different isolates were 5.14 h ± 1.47 h at 37 °C and 5.55 h ± 1.87 h at 40 °C. This is the first report to underscore the predominance of thermotolerant and delayed doubling time phenotypes in H. capsulatum clinical isolates from different regions of Latin America. Elsevier 2020-01-25 /pmc/articles/PMC9392019/ /pubmed/31987782 http://dx.doi.org/10.1016/j.bjid.2019.12.007 Text en © 2020 Published by Elsevier España, S.L.U. on behalf of Sociedade Brasileira de Infectologia. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Sahaza, Jorge H. Rodríguez-Arellanez, Gabriela Canteros, Cristina E. Reyes-Montes, María del Rocío Taylor, Maria Lucia Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions |
title | Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions |
title_full | Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions |
title_fullStr | Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions |
title_full_unstemmed | Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions |
title_short | Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions |
title_sort | thermotolerance of histoplasma capsulatum at 40 °c predominates among clinical isolates from different latin american regions |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9392019/ https://www.ncbi.nlm.nih.gov/pubmed/31987782 http://dx.doi.org/10.1016/j.bjid.2019.12.007 |
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