Enzymatic basis of the Fc-selective intra-chain disulfide reduction and free thiol content variability in an antibody produced in Escherichia coli

BACKGROUND: Escherichia coli (E. coli) is a promising host for production of recombinant proteins (including antibodies and antibody fragments) that don’t require complex post-translational modifications such as glycosylation. During manufacturing-scale production of a one-armed antibody in E. coli...

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Detalles Bibliográficos
Autores principales: Baginski, Tomasz K., Veeravalli, Karthik, McKenna, Rebekah, Williams, Christopher, Wong, Katherine, Tsai, Christina, Hewitt, Daniel, Mani, Karthik, Laird, Michael W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9392285/
https://www.ncbi.nlm.nih.gov/pubmed/35986313
http://dx.doi.org/10.1186/s12934-022-01892-4

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