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The greening reaction of skipjack tuna (Katsuwonus pelamis) metmyoglobin promoted by free cysteine during thermal treatment

BACKGROUND: Tuna muscle greening is a problem that occurs after heating. A hypothesis has been postulated to address this problem, involving a conserved Cys residue at position 10 (Cys-10) present on tuna myoglobin (Mb) that is exposed during the thermic treatment, forming a disulfide bond with free...

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Autores principales: Álvarez-Armenta, Andrés, Pacheco-Aguilar, Ramón, López-Zavala, Alonso A., Corona-Martínez, David O., Sotelo-Mundo, Rogerio R., García-Orozco, Karina D., Ramírez-Suárez, Juan C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9392451/
https://www.ncbi.nlm.nih.gov/pubmed/35996665
http://dx.doi.org/10.7717/peerj.13923
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author Álvarez-Armenta, Andrés
Pacheco-Aguilar, Ramón
López-Zavala, Alonso A.
Corona-Martínez, David O.
Sotelo-Mundo, Rogerio R.
García-Orozco, Karina D.
Ramírez-Suárez, Juan C.
author_facet Álvarez-Armenta, Andrés
Pacheco-Aguilar, Ramón
López-Zavala, Alonso A.
Corona-Martínez, David O.
Sotelo-Mundo, Rogerio R.
García-Orozco, Karina D.
Ramírez-Suárez, Juan C.
author_sort Álvarez-Armenta, Andrés
collection PubMed
description BACKGROUND: Tuna muscle greening is a problem that occurs after heating. A hypothesis has been postulated to address this problem, involving a conserved Cys residue at position 10 (Cys-10) present on tuna myoglobin (Mb) that is exposed during the thermic treatment, forming a disulfide bond with free cysteine (Cys) in the presence of trimethylamine oxide (TMAO), resulting in the greening of the tuna Mb. METHODS: We present a study using skipjack tuna (Katsuwonus pelamis) metmyoglobin (MbFe(III)-H(2)O) where the effect of free Cys (1–6 mM), TMAO (1.33 mM), and catalase on the greening reaction (GR) was monitored by UV-vis spectrometry during thermal treatment at 60 °C for 30 min. Moreover, the participation of Cys-10 on the GR was evaluated after its blocking with N-ethymaleimide. RESULTS: The GR occurred in tuna MbFe(III)-H(2)O after heat treatment with free Cys, forming sulfmyoglobin (MbFe(II)-S) as the responsible pigment for the tuna greening. However, the rate constants of MbFe(II)-S production depended on Cys concentration (up to 4 mM) and occurred regardless of the TMAO presence. We postulate that two consecutive reactions involve an intermediate ferrylmyoglobin (promoted by H(2)O(2)) species with a subsequent MbFe(II)-S formation since the presence of catalase fosters the reduction of the rate reaction. Moreover, GR occurred even with blocked Cys-10 residues in tuna Mb and horse Mb (without Cys in its sequence). DISCUSSION: We found that GR is not exclusive to tuna Mb´s, and it can be promoted in other muscle systems. Moreover, Cys and thermal treatment are indispensable for promoting this pigmentation anomaly.
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spelling pubmed-93924512022-08-21 The greening reaction of skipjack tuna (Katsuwonus pelamis) metmyoglobin promoted by free cysteine during thermal treatment Álvarez-Armenta, Andrés Pacheco-Aguilar, Ramón López-Zavala, Alonso A. Corona-Martínez, David O. Sotelo-Mundo, Rogerio R. García-Orozco, Karina D. Ramírez-Suárez, Juan C. PeerJ Aquaculture, Fisheries and Fish Science BACKGROUND: Tuna muscle greening is a problem that occurs after heating. A hypothesis has been postulated to address this problem, involving a conserved Cys residue at position 10 (Cys-10) present on tuna myoglobin (Mb) that is exposed during the thermic treatment, forming a disulfide bond with free cysteine (Cys) in the presence of trimethylamine oxide (TMAO), resulting in the greening of the tuna Mb. METHODS: We present a study using skipjack tuna (Katsuwonus pelamis) metmyoglobin (MbFe(III)-H(2)O) where the effect of free Cys (1–6 mM), TMAO (1.33 mM), and catalase on the greening reaction (GR) was monitored by UV-vis spectrometry during thermal treatment at 60 °C for 30 min. Moreover, the participation of Cys-10 on the GR was evaluated after its blocking with N-ethymaleimide. RESULTS: The GR occurred in tuna MbFe(III)-H(2)O after heat treatment with free Cys, forming sulfmyoglobin (MbFe(II)-S) as the responsible pigment for the tuna greening. However, the rate constants of MbFe(II)-S production depended on Cys concentration (up to 4 mM) and occurred regardless of the TMAO presence. We postulate that two consecutive reactions involve an intermediate ferrylmyoglobin (promoted by H(2)O(2)) species with a subsequent MbFe(II)-S formation since the presence of catalase fosters the reduction of the rate reaction. Moreover, GR occurred even with blocked Cys-10 residues in tuna Mb and horse Mb (without Cys in its sequence). DISCUSSION: We found that GR is not exclusive to tuna Mb´s, and it can be promoted in other muscle systems. Moreover, Cys and thermal treatment are indispensable for promoting this pigmentation anomaly. PeerJ Inc. 2022-08-17 /pmc/articles/PMC9392451/ /pubmed/35996665 http://dx.doi.org/10.7717/peerj.13923 Text en © 2022 Álvarez-Armenta et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Aquaculture, Fisheries and Fish Science
Álvarez-Armenta, Andrés
Pacheco-Aguilar, Ramón
López-Zavala, Alonso A.
Corona-Martínez, David O.
Sotelo-Mundo, Rogerio R.
García-Orozco, Karina D.
Ramírez-Suárez, Juan C.
The greening reaction of skipjack tuna (Katsuwonus pelamis) metmyoglobin promoted by free cysteine during thermal treatment
title The greening reaction of skipjack tuna (Katsuwonus pelamis) metmyoglobin promoted by free cysteine during thermal treatment
title_full The greening reaction of skipjack tuna (Katsuwonus pelamis) metmyoglobin promoted by free cysteine during thermal treatment
title_fullStr The greening reaction of skipjack tuna (Katsuwonus pelamis) metmyoglobin promoted by free cysteine during thermal treatment
title_full_unstemmed The greening reaction of skipjack tuna (Katsuwonus pelamis) metmyoglobin promoted by free cysteine during thermal treatment
title_short The greening reaction of skipjack tuna (Katsuwonus pelamis) metmyoglobin promoted by free cysteine during thermal treatment
title_sort greening reaction of skipjack tuna (katsuwonus pelamis) metmyoglobin promoted by free cysteine during thermal treatment
topic Aquaculture, Fisheries and Fish Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9392451/
https://www.ncbi.nlm.nih.gov/pubmed/35996665
http://dx.doi.org/10.7717/peerj.13923
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