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Tris(methylthio)methane produced by Mortierella hyalina affects sulfur homeostasis in Arabidopsis
Microbial volatiles are important factors in symbiotic interactions with plants. Mortierella hyalina is a beneficial root-colonizing fungus with a garlic-like smell, and promotes growth of Arabidopsis seedlings. GC–MS analysis of the M. hyalina headspace and NMR analysis of the extracted essential o...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9392766/ https://www.ncbi.nlm.nih.gov/pubmed/35987806 http://dx.doi.org/10.1038/s41598-022-16827-7 |
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author | Tseng, Y.-H. Bartram, S. Reichelt, M. Scholz, S. S. Meents, A. K. Ludwig, A. Mithöfer, A. Oelmüller, R. |
author_facet | Tseng, Y.-H. Bartram, S. Reichelt, M. Scholz, S. S. Meents, A. K. Ludwig, A. Mithöfer, A. Oelmüller, R. |
author_sort | Tseng, Y.-H. |
collection | PubMed |
description | Microbial volatiles are important factors in symbiotic interactions with plants. Mortierella hyalina is a beneficial root-colonizing fungus with a garlic-like smell, and promotes growth of Arabidopsis seedlings. GC–MS analysis of the M. hyalina headspace and NMR analysis of the extracted essential oil identified the sulfur-containing volatile tris(methylthio)methane (TMTM) as the major compound. Incorporation of the sulfur from the fungal volatile into plant metabolism was shown by (34)S labeling experiments. Under sulfur deficiency, TMTM down-regulated sulfur deficiency-responsive genes, prevented glucosinolate (GSL) and glutathione (GSH) diminishment, and sustained plant growth. However, excess TMTM led to accumulation of GSH and GSL and reduced plant growth. Since TMTM is not directly incorporated into cysteine, we propose that the volatile from M. hyalina influences the plant sulfur metabolism by interfering with the GSH metabolism, and alleviates sulfur imbalances under sulfur stress. |
format | Online Article Text |
id | pubmed-9392766 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-93927662022-08-22 Tris(methylthio)methane produced by Mortierella hyalina affects sulfur homeostasis in Arabidopsis Tseng, Y.-H. Bartram, S. Reichelt, M. Scholz, S. S. Meents, A. K. Ludwig, A. Mithöfer, A. Oelmüller, R. Sci Rep Article Microbial volatiles are important factors in symbiotic interactions with plants. Mortierella hyalina is a beneficial root-colonizing fungus with a garlic-like smell, and promotes growth of Arabidopsis seedlings. GC–MS analysis of the M. hyalina headspace and NMR analysis of the extracted essential oil identified the sulfur-containing volatile tris(methylthio)methane (TMTM) as the major compound. Incorporation of the sulfur from the fungal volatile into plant metabolism was shown by (34)S labeling experiments. Under sulfur deficiency, TMTM down-regulated sulfur deficiency-responsive genes, prevented glucosinolate (GSL) and glutathione (GSH) diminishment, and sustained plant growth. However, excess TMTM led to accumulation of GSH and GSL and reduced plant growth. Since TMTM is not directly incorporated into cysteine, we propose that the volatile from M. hyalina influences the plant sulfur metabolism by interfering with the GSH metabolism, and alleviates sulfur imbalances under sulfur stress. Nature Publishing Group UK 2022-08-20 /pmc/articles/PMC9392766/ /pubmed/35987806 http://dx.doi.org/10.1038/s41598-022-16827-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Tseng, Y.-H. Bartram, S. Reichelt, M. Scholz, S. S. Meents, A. K. Ludwig, A. Mithöfer, A. Oelmüller, R. Tris(methylthio)methane produced by Mortierella hyalina affects sulfur homeostasis in Arabidopsis |
title | Tris(methylthio)methane produced by Mortierella hyalina affects sulfur homeostasis in Arabidopsis |
title_full | Tris(methylthio)methane produced by Mortierella hyalina affects sulfur homeostasis in Arabidopsis |
title_fullStr | Tris(methylthio)methane produced by Mortierella hyalina affects sulfur homeostasis in Arabidopsis |
title_full_unstemmed | Tris(methylthio)methane produced by Mortierella hyalina affects sulfur homeostasis in Arabidopsis |
title_short | Tris(methylthio)methane produced by Mortierella hyalina affects sulfur homeostasis in Arabidopsis |
title_sort | tris(methylthio)methane produced by mortierella hyalina affects sulfur homeostasis in arabidopsis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9392766/ https://www.ncbi.nlm.nih.gov/pubmed/35987806 http://dx.doi.org/10.1038/s41598-022-16827-7 |
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