Cargando…
Differential biological responses of adherent and non-adherent (cancer and non-cancerous) cells to variable extremely low frequency magnetic fields
Extremely low-frequency electromagnetic field (ELF-EMF) induces biological effects on different cells through various signaling pathways. To study the impact of the ELF-EMF on living cells under an optimal physiological condition, we have designed and constructed a novel system that eliminates sever...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9392794/ https://www.ncbi.nlm.nih.gov/pubmed/35987807 http://dx.doi.org/10.1038/s41598-022-18210-y |
_version_ | 1784771142850969600 |
---|---|
author | Nezamtaheri, Maryam Sadat Goliaei, Bahram Shariatpanahi, Seyed Peyman Ansari, Alireza Madjid |
author_facet | Nezamtaheri, Maryam Sadat Goliaei, Bahram Shariatpanahi, Seyed Peyman Ansari, Alireza Madjid |
author_sort | Nezamtaheri, Maryam Sadat |
collection | PubMed |
description | Extremely low-frequency electromagnetic field (ELF-EMF) induces biological effects on different cells through various signaling pathways. To study the impact of the ELF-EMF on living cells under an optimal physiological condition, we have designed and constructed a novel system that eliminates several limitations of other ELF-EMF systems. Apoptosis and cell number were assessed by flow cytometry and the Trypan Blue dye exclusion method, respectively. In vitro cell survival was evaluated by colony formation assay. The distribution of cells in the cell cycle, intracellular ROS level, and autophagy were analyzed by flow cytometer. Suspended cells differentiation was assessed by phagocytosis of latex particles and NBT reduction assay. Our results showed that response to the exposure to ELF-EMF is specific and depends on the biological state of the cell. For DU145, HUVEC, and K562 cell lines the optimum results were obtained at the frequency of 0.01 Hz, while for MDA-MB-231, the optimum response was obtained at 1 Hz. Long-term exposure to ELF-EMF in adherent cells effectively inhibited proliferation by arresting the cell population at the cell cycle G2/M phase and increased intracellular ROS level, leading to morphological changes and cell death. The K562 cells exposed to the ELF-EMF differentiate via induction of autophagy and decreasing the cell number. Our novel ELF-EMF instrument could change morphological and cell behaviors, including proliferation, differentiation, and cell death. |
format | Online Article Text |
id | pubmed-9392794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-93927942022-08-22 Differential biological responses of adherent and non-adherent (cancer and non-cancerous) cells to variable extremely low frequency magnetic fields Nezamtaheri, Maryam Sadat Goliaei, Bahram Shariatpanahi, Seyed Peyman Ansari, Alireza Madjid Sci Rep Article Extremely low-frequency electromagnetic field (ELF-EMF) induces biological effects on different cells through various signaling pathways. To study the impact of the ELF-EMF on living cells under an optimal physiological condition, we have designed and constructed a novel system that eliminates several limitations of other ELF-EMF systems. Apoptosis and cell number were assessed by flow cytometry and the Trypan Blue dye exclusion method, respectively. In vitro cell survival was evaluated by colony formation assay. The distribution of cells in the cell cycle, intracellular ROS level, and autophagy were analyzed by flow cytometer. Suspended cells differentiation was assessed by phagocytosis of latex particles and NBT reduction assay. Our results showed that response to the exposure to ELF-EMF is specific and depends on the biological state of the cell. For DU145, HUVEC, and K562 cell lines the optimum results were obtained at the frequency of 0.01 Hz, while for MDA-MB-231, the optimum response was obtained at 1 Hz. Long-term exposure to ELF-EMF in adherent cells effectively inhibited proliferation by arresting the cell population at the cell cycle G2/M phase and increased intracellular ROS level, leading to morphological changes and cell death. The K562 cells exposed to the ELF-EMF differentiate via induction of autophagy and decreasing the cell number. Our novel ELF-EMF instrument could change morphological and cell behaviors, including proliferation, differentiation, and cell death. Nature Publishing Group UK 2022-08-20 /pmc/articles/PMC9392794/ /pubmed/35987807 http://dx.doi.org/10.1038/s41598-022-18210-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Nezamtaheri, Maryam Sadat Goliaei, Bahram Shariatpanahi, Seyed Peyman Ansari, Alireza Madjid Differential biological responses of adherent and non-adherent (cancer and non-cancerous) cells to variable extremely low frequency magnetic fields |
title | Differential biological responses of adherent and non-adherent (cancer and non-cancerous) cells to variable extremely low frequency magnetic fields |
title_full | Differential biological responses of adherent and non-adherent (cancer and non-cancerous) cells to variable extremely low frequency magnetic fields |
title_fullStr | Differential biological responses of adherent and non-adherent (cancer and non-cancerous) cells to variable extremely low frequency magnetic fields |
title_full_unstemmed | Differential biological responses of adherent and non-adherent (cancer and non-cancerous) cells to variable extremely low frequency magnetic fields |
title_short | Differential biological responses of adherent and non-adherent (cancer and non-cancerous) cells to variable extremely low frequency magnetic fields |
title_sort | differential biological responses of adherent and non-adherent (cancer and non-cancerous) cells to variable extremely low frequency magnetic fields |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9392794/ https://www.ncbi.nlm.nih.gov/pubmed/35987807 http://dx.doi.org/10.1038/s41598-022-18210-y |
work_keys_str_mv | AT nezamtaherimaryamsadat differentialbiologicalresponsesofadherentandnonadherentcancerandnoncancerouscellstovariableextremelylowfrequencymagneticfields AT goliaeibahram differentialbiologicalresponsesofadherentandnonadherentcancerandnoncancerouscellstovariableextremelylowfrequencymagneticfields AT shariatpanahiseyedpeyman differentialbiologicalresponsesofadherentandnonadherentcancerandnoncancerouscellstovariableextremelylowfrequencymagneticfields AT ansarialirezamadjid differentialbiologicalresponsesofadherentandnonadherentcancerandnoncancerouscellstovariableextremelylowfrequencymagneticfields |